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Literature summary extracted from

  • Cha, C.
    Catechol 1,2-dioxygenase from Rhodococcus rhodochrous N75 capable of metabolizing alkyl-substituted catechols (2006), J. Microbiol. Biotechnol., 16, 778-785.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.13.11.1 HgCl2 1 mM, 52% inhibition Rhodococcus rhodochrous
1.13.11.1 PCMB 0.1 mM, 16% inhibition Rhodococcus rhodochrous
1.13.11.1 Sodium dithionite 1 mM, 36% loss of activity Rhodococcus rhodochrous
1.13.11.1 Tiron 0.5 mM, 20% loss of activity Rhodococcus rhodochrous

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.1 additional information
-
 additional information
-
 Rhodococcus rhodochrous

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.13.11.1 35000
-
 2 * 35000, SDS-PAGE Rhodococcus rhodochrous
1.13.11.1 71000
-
 gel filtration Rhodococcus rhodochrous

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.1 Rhodococcus rhodochrous
-
-
-
1.13.11.1 Rhodococcus rhodochrous N75
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.1
-
 Rhodococcus rhodochrous

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.1 3,4-dimethylcatechol + O2 21% of activity with catechol Rhodococcus rhodochrous 3,4-dimethyl-cis,cis-muconate
-
 ?
1.13.11.1 3,4-dimethylcatechol + O2 21% of activity with catechol Rhodococcus rhodochrous N75 3,4-dimethyl-cis,cis-muconate
-
 ?
1.13.11.1 3,5-dimethylcatechol + O2 36% of activity with catechol Rhodococcus rhodochrous 3,5-dimethyl-cis,cis-muconate
-
 ?
1.13.11.1 3,5-dimethylcatechol + O2 36% of activity with catechol Rhodococcus rhodochrous N75 3,5-dimethyl-cis,cis-muconate
-
 ?
1.13.11.1 3-methylcatechol + O2 64% of activity with catechol Rhodococcus rhodochrous 2-methyl-cis,cis-muconate
-
 ?
1.13.11.1 3-methylcatechol + O2 64% of activity with catechol Rhodococcus rhodochrous N75 2-methyl-cis,cis-muconate
-
 ?
1.13.11.1 4-chlorocatechol + O2
-
 Rhodococcus rhodochrous 4-chloro-cis,cis-muconate
-
 ?
1.13.11.1 4-ethylcatechol + O2 42% of activity with catechol Rhodococcus rhodochrous 4-ethyl-cis-cis-muconate
-
 ?
1.13.11.1 4-fluorocatechol + O2 65% of activity with catechol Rhodococcus rhodochrous 4-fluoro-cis,cis-muconate
-
 ?
1.13.11.1 4-iso-propylcatechol + O2 23% of activity with catechol Rhodococcus rhodochrous 4-isopropyl-cis,cis-muconate
-
 ?
1.13.11.1 4-methylcatechol + O2 76% of activity with catechol Rhodococcus rhodochrous 3-methyl-cis,cis-muconate
-
 ?
1.13.11.1 4-methylcatechol + O2 76% of activity with catechol Rhodococcus rhodochrous N75 3-methyl-cis,cis-muconate
-
 ?
1.13.11.1 4-n-propylcatechol + O2 17% of activity with catechol Rhodococcus rhodochrous 4-n-propyl-cis,cis-muconate
-
 ?
1.13.11.1 catechol + O2
-
 Rhodococcus rhodochrous cis,cis-muconate
-
 ?
1.13.11.1 catechol + O2
-
 Rhodococcus rhodochrous N75 cis,cis-muconate
-
 ?

Subunits

EC Number Subunits Comment Organism
1.13.11.1 dimer 2 * 35000, SDS-PAGE Rhodococcus rhodochrous

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.13.11.1 55
-
 half-life of the enzyme from benzoate-grown cells is 27 min, half-life of the enzyme from p-toluate-grown cells is 14 min Rhodococcus rhodochrous

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.11.1 8
-
 in 50 mM Bis-Tris-propane buffer and 50 mM Tris-HCl buffer Rhodococcus rhodochrous

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.13.11.1 7 9 high activity Rhodococcus rhodochrous