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Literature summary extracted from

  • Lima, L.; Seabra, A.; Melo, P.; Cullimore, J.; Carvalho, H.
    Post-translational regulation of cytosolic glutamine synthetase of Medicago truncatula (2006), J. Exp. Bot., 57, 2751-2761.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.1.2 expressed in Escherichia coli Medicago truncatula

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.1.2 1.8
-
 L-Glu phosphorylated GS1a, in the presence of 0.0005 mM microcystin Medicago truncatula
6.3.1.2 2.8
-
 L-Glu non-phosphorylated GS1a, in the presence of 0.0005 mM microcystin Medicago truncatula
6.3.1.2 2.9
-
 ATP phosphorylated GS1a, in the presence of 0.0005 mM microcystin Medicago truncatula
6.3.1.2 3
-
 ATP non-phosphorylated GS1a, in the presence of 0.0005 mM microcystin Medicago truncatula

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
6.3.1.2 cytosol
-
 Medicago truncatula 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.3.1.2 40000
-
 SDS-PAGE, GS2 Medicago truncatula
6.3.1.2 41500
-
 SDS-PAGE, His-tagged GS1a Medicago truncatula

Organism

EC Number Organism UniProt Comment Textmining
6.3.1.2 Medicago truncatula
-
 cultivar Jemalong J5
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
6.3.1.2 phosphoprotein phosphorylation in root nodules Medicago truncatula

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.1.2 Ni-NTA affinity chromatography and P10 Sephadex column chromatography Medicago truncatula

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.3.1.2 leaf
-
 Medicago truncatula
-
6.3.1.2 nodule
-
 Medicago truncatula
-
6.3.1.2 root
-
 Medicago truncatula
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.1.2 ATP + L-Glu + NH4+
-
 Medicago truncatula ADP + phosphate + L-Gln
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.3.1.2 Glutamine synthetase
-
 Medicago truncatula
6.3.1.2 GS1 kinase
-
 Medicago truncatula
6.3.1.2 GS1a
-
 Medicago truncatula
6.3.1.2 GS2
-
 Medicago truncatula