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Literature summary extracted from

  • Zheng, X.; Morrison, A.R.; Chung, A.S.; Moss, J.; Bortell, R.
    Substrate specificity of soluble and membrane-associated ADP-ribosyltransferase ART2.1 (2006), J. Cell. Biochem., 98, 851-860.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.2.31 DTT dependent on, the DTT cencentration influences the target specificity of ART2 Mus musculus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.2.31 expression of His-tagged ART2 as maltose-binding fusion protein in Escherichia coli strain BL21(DE3), expression of the membrane-bound ART2 in COS1 cells Mus musculus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.2.31 ADP-ribose partial competitive inhibition Mus musculus
2.4.2.31 Triton X-100 detergent solubilization of cell membranes specifically abolishes ADP-ribosylation of high MW histones Mus musculus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.2.31 cell surface ART2 is attached to the cell surface by a glycosylphosphatidylinositol anchor in T-cells Mus musculus 9986
-
2.4.2.31 extracellular serum Mus musculus
-
-
2.4.2.31 membrane
-
 Mus musculus 16020
-
2.4.2.31 additional information the membrane or solution environment of ART2 plays a pivotal role in determining its substrate specificity Mus musculus
-
-
2.4.2.31 soluble
-
 Mus musculus
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.2.31 histone + NAD+ Mus musculus isozyme ART2 is active with high and low molecular weight histones, membrane-bound and PI-PLC released ART2 preferentially ADP-ribosylate high or low MW histones, respectively Nomega-[(2'-phospho-ADP)-D-ribosyl]-histone-L-arginine + nicotinamide + H+
-
 ?
2.4.2.31 additional information Mus musculus protein mono-ADP-ribosylation is a reversible post-translational modification that plays a role in regulation of cellular activities specific amino acid of the acceptor protein, overview ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.31 Mus musculus
-
 isozyme ART2
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.2.31 recombinant membrane-bound His-tagged ART2 maltose-binding fusion protein from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, anion echange chromatography, and amylose resin affnity chromataography, the maltose-binding protein is cleaved off Mus musculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.2.31 serum
-
 Mus musculus
-
2.4.2.31 T-lymphocyte ART2 is a T-cell-specific transferase Mus musculus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.31 histone + NAD+ isozyme ART2 is active with high and low molecular weight histones, membrane-bound and PI-PLC released ART2 preferentially ADP-ribosylate high or low MW histones, respectively Mus musculus Nomega-[(2'-phospho-ADP)-D-ribosyl]-histone-L-arginine + nicotinamide + H+
-
 ?
2.4.2.31 additional information protein mono-ADP-ribosylation is a reversible post-translational modification that plays a role in regulation of cellular activities specific amino acid of the acceptor protein, overview Mus musculus ?
-
 ?
2.4.2.31 additional information ART2, in the absence of histone acceptor, has NAD+ glycohydrolase activity, overview, serum proteins are ADP-ribosylated in a thiol-specific manner, soluble recombinant ART2 lacking the GPI anchor shows a different histone specificity than does native cell-bound ART2, the membrane or solution environment of ART2 plays a pivotal role in determining its substrate specificity Mus musculus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.2.31 ART
-
 Mus musculus
2.4.2.31 mono-ADP-ribosyltransferase
-
 Mus musculus
2.4.2.31 NAD:arginine ADP-ribosyltransferase
-
 Mus musculus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.2.31 37
-
 assay at Mus musculus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.2.31 7.6
-
 assay at Mus musculus