Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Evrin, C.; Straut, M.; Slavova-Azmanova, N.; Bucurenci, N.; Onu, A.; Assairi, L.; Ionescu, M.; Palibroda, N.; Barzu, O.; Gilles, A.
    Regulatory mechanisms differ in UMP kinases from Gram-negative and Gram-positive bacteria (2007), J. Biol. Chem., 282, 7242-7253.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.4.22 3'-anthraniloyl-dGTP
-
 Haemophilus influenzae
2.7.4.22 3'-anthraniloyl-dGTP
-
 Escherichia coli
2.7.4.22 3'-anthraniloyl-dGTP
-
 Neisseria meningitidis
2.7.4.22 3'-anthraniloyl-dGTP strong activator Streptococcus pneumoniae
2.7.4.22 3'-anthraniloyl-dGTP strong activator Bacillus subtilis
2.7.4.22 7-deaza-dGTP
-
 Haemophilus influenzae
2.7.4.22 7-deaza-dGTP
-
 Streptococcus pneumoniae
2.7.4.22 7-deaza-dGTP
-
 Bacillus subtilis
2.7.4.22 7-deaza-dGTP
-
 Escherichia coli
2.7.4.22 7-deaza-dGTP
-
 Neisseria meningitidis
2.7.4.22 dGTP
-
 Haemophilus influenzae
2.7.4.22 dGTP
-
 Streptococcus pneumoniae
2.7.4.22 dGTP
-
 Bacillus subtilis
2.7.4.22 dGTP
-
 Escherichia coli
2.7.4.22 dGTP
-
 Neisseria meningitidis
2.7.4.22 GMP
-
 Haemophilus influenzae
2.7.4.22 GMP not activating Streptococcus pneumoniae
2.7.4.22 GMP not activating Bacillus subtilis
2.7.4.22 GMP-PNP
-
 Streptococcus pneumoniae
2.7.4.22 GMP-PNP
-
 Bacillus subtilis
2.7.4.22 GMP-PNP
-
 Escherichia coli
2.7.4.22 GMP-PNP GMP-PNP Haemophilus influenzae
2.7.4.22 GMP-PNP less activating Neisseria meningitidis
2.7.4.22 GTP 0.5 mM Staphylococcus aureus
2.7.4.22 GTP 0.5 mM Enterococcus faecalis
2.7.4.22 GTP 0.5 mM Streptococcus pneumoniae
2.7.4.22 GTP at 0.5 mM Salmonella enterica subsp. enterica serovar Typhimurium
2.7.4.22 GTP 0.5 mM, independent of the concentrations of Mg2+ Haemophilus influenzae
2.7.4.22 GTP 0.5 mM, more activating at low concentrations of ATP Bacillus subtilis
2.7.4.22 GTP 0.5 mM, reverses the inhibition of excess UMP, it increases the affinity for ATP Escherichia coli
2.7.4.22 GTP 0.5 mM, reverses the inhibition of excess UMP, it increases the affinity for ATP Neisseria meningitidis
2.7.4.22 ITP
-
 Haemophilus influenzae
2.7.4.22 ITP
-
 Streptococcus pneumoniae
2.7.4.22 ITP
-
 Bacillus subtilis
2.7.4.22 ITP
-
 Escherichia coli
2.7.4.22 ITP
-
 Neisseria meningitidis
2.7.4.22 additional information GMP is not activating Neisseria meningitidis
2.7.4.22 XTP
-
 Haemophilus influenzae
2.7.4.22 XTP
-
 Streptococcus pneumoniae
2.7.4.22 XTP
-
 Bacillus subtilis
2.7.4.22 XTP
-
 Escherichia coli
2.7.4.22 XTP
-
 Neisseria meningitidis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.4.22 expression in Escherichia coli Salmonella enterica subsp. enterica serovar Typhimurium
2.7.4.22 expression in Escherichia coli Staphylococcus aureus
2.7.4.22 expression in Escherichia coli Haemophilus influenzae
2.7.4.22 expression in Escherichia coli Enterococcus faecalis
2.7.4.22 expression in Escherichia coli Streptococcus pneumoniae
2.7.4.22 expression in Escherichia coli Neisseria meningitidis
2.7.4.22 expression in Escherichia coli, site directed mutagenesis Bacillus subtilis
2.7.4.22 expression in Escherichia coli, site directed mutagenesis Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
2.7.4.22 N137A Tm 10°C lower than wild type, loss of cooperativity with ATP, sensitive to activation by GTP Bacillus subtilis
2.7.4.22 N140A cooperative inhibition by GTP and UTP is altered, lower thermodynamic stability Escherichia coli
2.7.4.22 T135A Tm 10°C lower than wild type, loss of cooperativity with ATP, increase in Km for UMP, sensitive to activation by GTP Bacillus subtilis
2.7.4.22 T135A/N137A Tm 10°C lower than wild type, at pH 7.4 in 50 mM Tris irreversible inactivated within hours Bacillus subtilis

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.4.22 5-bromo-UMP not inhibitory Enterococcus faecalis
2.7.4.22 5-bromo-UMP not inhibitory Escherichia coli
2.7.4.22 5-bromo-UMP not inhibitory Haemophilus influenzae
2.7.4.22 5-bromo-UMP not inhibitory Streptococcus pneumoniae
2.7.4.22 5-Bromo-UTP
-
 Bacillus subtilis
2.7.4.22 5-Bromo-UTP less inhibitory than UTP Enterococcus faecalis
2.7.4.22 5-Bromo-UTP less inhibitory than UTP Escherichia coli
2.7.4.22 5-Bromo-UTP less inhibitory than UTP Haemophilus influenzae
2.7.4.22 5-Bromo-UTP more inhibitory than UTP Streptococcus pneumoniae
2.7.4.22 5-fluoro-UTP
-
 Bacillus subtilis
2.7.4.22 5-fluoro-UTP like UTP Enterococcus faecalis
2.7.4.22 5-fluoro-UTP like UTP Escherichia coli
2.7.4.22 5-fluoro-UTP like UTP Haemophilus influenzae
2.7.4.22 5-fluoro-UTP less inhibitory than UTP Streptococcus pneumoniae
2.7.4.22 5-iodo-UMP not inhibitory Enterococcus faecalis
2.7.4.22 5-iodo-UMP not inhibitory Escherichia coli
2.7.4.22 5-iodo-UMP not inhibitory Haemophilus influenzae
2.7.4.22 5-iodo-UMP not inhibitory Streptococcus pneumoniae
2.7.4.22 5-iodo-UTP
-
 Bacillus subtilis
2.7.4.22 5-iodo-UTP less inhibitory than UTP Enterococcus faecalis
2.7.4.22 5-iodo-UTP less inhibitory than UTP Escherichia coli
2.7.4.22 5-iodo-UTP less inhibitory than UTP Haemophilus influenzae
2.7.4.22 5-iodo-UTP more inhibitory than UTP Streptococcus pneumoniae
2.7.4.22 dUTP five times weaker than UTP Bacillus subtilis
2.7.4.22 dUTP five times weaker than UTP Escherichia coli
2.7.4.22 dUTP five times weaker than UTP Haemophilus influenzae
2.7.4.22 dUTP five times weaker than UTP Streptococcus pneumoniae
2.7.4.22 TTP not inhibitory Bacillus subtilis
2.7.4.22 TTP not inhibitory Enterococcus faecalis
2.7.4.22 TTP not inhibitory Escherichia coli
2.7.4.22 TTP not inhibitory Haemophilus influenzae
2.7.4.22 TTP not inhibitory Streptococcus pneumoniae
2.7.4.22 UMP
-
 Bacillus subtilis
2.7.4.22 UMP excess UMP at high concentrations, UTP deminishes this inhibition Escherichia coli
2.7.4.22 UMP excess UMP at high concentrations, UTP deminishes this inhibition Haemophilus influenzae
2.7.4.22 UMP excess UMP at high concentrations, UTP deminishes this inhibition Neisseria meningitidis
2.7.4.22 UMP excess UMP at high concentrations Streptococcus pneumoniae
2.7.4.22 UTP inhibition not affected by Mg2+ and UMP Bacillus subtilis
2.7.4.22 UTP inhibition not affected by Mg2+ and UMP Enterococcus faecalis
2.7.4.22 UTP 0.25 mM, 0.5 mM, GTP, UMP and high concentrations of Mg2+ protects against inhibition by UTP, decreases the affinity for ATP, inhibition only without Mg2+ Escherichia coli
2.7.4.22 UTP 0.25 mM, 0.5 mM, GTP, UMP and high concentrations of Mg2+ protects against inhibition by UTP, decreases the affinity for ATP, inhibition only without Mg2+ Haemophilus influenzae
2.7.4.22 UTP 0.25 mM, 0.5 mM, UMP protects against inhibition by UTP, decreases the affinity for ATP, inhibition only without Mg2+ Neisseria meningitidis
2.7.4.22 UTP
-
 Streptococcus pneumoniae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.4.22 0.0087
-
 UMP in presence of 2.0 mM ATP without GTP Neisseria meningitidis
2.7.4.22 0.01
-
 UMP in presence of 2.0 mM ATP without GTP Bacillus subtilis
2.7.4.22 0.0156
-
 UMP in presence of 10.0 mM ATP without GTP Neisseria meningitidis
2.7.4.22 0.0274
-
 UMP in presence of 2.0 mM ATP and 0.5 mM GTP Bacillus subtilis
2.7.4.22 0.04
-
 UMP in presence of 1.0 mM ATP and 0.5 mM GTP Haemophilus influenzae
2.7.4.22 0.04
-
 UMP in presence of 1.0 mM ATP without GTP Haemophilus influenzae
2.7.4.22 0.046
-
 UMP in presence of 2.0 mM ATP without GTP Escherichia coli
2.7.4.22 0.047
-
 UMP in presence of 0.2 mM ATP and 0.5 mM GTP Escherichia coli
2.7.4.22 0.0492
-
 UMP in presence of 0.2 mM ATP without GTP Escherichia coli
2.7.4.22 0.05
-
 UMP in presence of 12.0 mM ATP and 0.5 mM GTP Haemophilus influenzae
2.7.4.22 0.051
-
 UMP in presence of 2.0 mM ATP and 0.5 mM GTP Escherichia coli
2.7.4.22 0.0574
-
 UMP in presence of 10.0 mM ATP and 0.5 mM GTP Neisseria meningitidis
2.7.4.22 0.0997
-
 UMP in presence of 2.0 mM ATP and 0.5 mM GTP Streptococcus pneumoniae
2.7.4.22 0.1
-
 UMP in presence of 12.0 mM ATP without GTP Haemophilus influenzae
2.7.4.22 0.1
-
 UMP in presence of 2.0 mM ATP and 0.5 mM GTP Neisseria meningitidis
2.7.4.22 0.1056
-
 UMP in presence of 30 mM ATP and 0.5 mM GTP Streptococcus pneumoniae
2.7.4.22 0.131
-
 UMP in presence of 30 mM ATP without GTP Bacillus subtilis
2.7.4.22 0.15
-
 ATP in presence of 1.0 mM UMP without GTP Escherichia coli
2.7.4.22 0.15
-
 UMP in presence of 30 mM ATP without GTP Streptococcus pneumoniae
2.7.4.22 0.155
-
 UMP in presence of 30 mM ATP and 0.5 mM GTP Bacillus subtilis
2.7.4.22 0.2
-
 ATP in presence of 1.0 mM UMP and 0.5 mM GTP Escherichia coli
2.7.4.22 0.207
-
 UMP in presence of 2.0 mM ATP, 0.5 mM UTP and 0.5 mM GTP Escherichia coli
2.7.4.22 0.23
-
 ATP in presence of 0.1 mM UMP without GTP Escherichia coli
2.7.4.22 0.26
-
 ATP in presence of 2.0 mM ATP, 0.5 mM UTP and 0.5 mM GTP Escherichia coli
2.7.4.22 0.27
-
 ATP in presence of 0.1 mM UMP and 0.5 mM GTP Escherichia coli
2.7.4.22 0.46
-
 ATP in presence of 0.1 mM UMP and 0.5 mM GTP Haemophilus influenzae
2.7.4.22 0.6
-
 ATP in presence of 1.0 mM UMP and 0.5 mM GTP Haemophilus influenzae
2.7.4.22 0.77
-
 ATP in presence of 2.0 mM ATP and 0.5 mM UTP Escherichia coli
2.7.4.22 0.83
-
 ATP in presence of 0.05 mM UMP and 0.5 mM GTP Neisseria meningitidis
2.7.4.22 0.9
-
 ATP in presence of 1.0 mM UMP and 0.5 mM GTP Staphylococcus aureus
2.7.4.22 0.92
-
 ATP in presence of 1.0 mM UMP and 0.5 mM GTP Streptococcus pneumoniae
2.7.4.22 0.93
-
 ATP in presence of 0.1 mM UMP and 0.5 mM GTP Bacillus subtilis
2.7.4.22 1
-
 ATP in presence of 0.5 mM UTP Escherichia coli
2.7.4.22 1.57
-
 ATP in presence of 0.1 mM UMP without GTP Haemophilus influenzae
2.7.4.22 1.62
-
 ATP in presence of 1.0 mM UMP without GTP Haemophilus influenzae
2.7.4.22 1.69
-
 UMP in presence of 2.0 mM ATP and 0.5 mM UTP Escherichia coli
2.7.4.22 1.79
-
 ATP in presence of 1.0 mM UMP and 0.5 mM GTP Bacillus subtilis
2.7.4.22 1.98
-
 ATP in presence of 1.0 mM UMP and 0.5 mM GTP Neisseria meningitidis
2.7.4.22 2.64
-
 ATP in presence of 1.0 mM UMP without GTP Staphylococcus aureus
2.7.4.22 2.98
-
 ATP in presence of 0.05 mM UMP without GTP Neisseria meningitidis
2.7.4.22 3.22
-
 ATP in presence of 1.0 mM UMP without GTP Neisseria meningitidis
2.7.4.22 4.5
-
 ATP in presence of 0.5 mM UTP Haemophilus influenzae
2.7.4.22 9.8
-
 ATP in presence of 1.0 mM UMP without GTP Streptococcus pneumoniae
2.7.4.22 10.4
-
 ATP in presence of 0.1 mM UMP without GTP Bacillus subtilis
2.7.4.22 14.5
-
 ATP in presence of 1.0 mM UMP without GTP Bacillus subtilis
2.7.4.22 22.2
-
 UMP in presence of 2.0 mM ATP without GTP Streptococcus pneumoniae
2.7.4.22 30
-
 ATP in presence of 0.5 mM UTP Bacillus subtilis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.4.22 Mg2+
-
 Salmonella enterica subsp. enterica serovar Typhimurium
2.7.4.22 Mg2+
-
 Staphylococcus aureus
2.7.4.22 Mg2+
-
 Haemophilus influenzae
2.7.4.22 Mg2+
-
 Enterococcus faecalis
2.7.4.22 Mg2+
-
 Streptococcus pneumoniae
2.7.4.22 Mg2+
-
 Bacillus subtilis
2.7.4.22 Mg2+
-
 Escherichia coli
2.7.4.22 Mg2+
-
 Neisseria meningitidis

Organism

EC Number Organism UniProt Comment Textmining
2.7.4.22 Bacillus subtilis O31749
-
-
2.7.4.22 Enterococcus faecalis
-
-
-
2.7.4.22 Escherichia coli A7ZWB7
-
-
2.7.4.22 Haemophilus influenzae
-
-
-
2.7.4.22 Neisseria meningitidis P65932
-
-
2.7.4.22 Salmonella enterica subsp. enterica serovar Typhimurium
-
-
-
2.7.4.22 Staphylococcus aureus
-
-
-
2.7.4.22 Streptococcus pneumoniae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.4.22 of the recombinant protein by affinity chromatography Salmonella enterica subsp. enterica serovar Typhimurium
2.7.4.22 of the recombinant protein by affinity chromatography Staphylococcus aureus
2.7.4.22 of the recombinant protein by affinity chromatography Enterococcus faecalis
2.7.4.22 of the recombinant protein by affinity chromatography Streptococcus pneumoniae
2.7.4.22 of the recombinant protein by affinity chromatography Bacillus subtilis
2.7.4.22 of the recombinant protein by affinity chromatography Neisseria meningitidis
2.7.4.22 of the recombinant protein by affinity chromatography, no difference in activity compared to the wild type Haemophilus influenzae
2.7.4.22 of the recombinant protein by affinity chromatography, no difference in activity compared to the wild type Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.4.22 1.7
-
 at 2.0 mM ATP, without GTP Enterococcus faecalis
2.7.4.22 2.7
-
 at 2.0 mM ATP, without GTP Bacillus subtilis
2.7.4.22 3.7
-
 at 2.0 mM ATP, without GTP Streptococcus pneumoniae
2.7.4.22 4.6
-
 at 2.0 mM ATP, without GTP Staphylococcus aureus
2.7.4.22 5.1
-
 activity dependent of concentrations of ATP, at 2.0 mM ATP, without GTP Neisseria meningitidis
2.7.4.22 9.1
-
 activity independent of concentrations of ATP, at 2.0 mM ATP, without GTP Haemophilus influenzae
2.7.4.22 9.1
-
 at 8.0 mM ATP, without GTP Enterococcus faecalis
2.7.4.22 9.7
-
 at 2.0 mM ATP, with 0.5 mM GTP Enterococcus faecalis
2.7.4.22 10.2
-
 at 8.0 mM ATP, with 0.5 mM GTP Neisseria meningitidis
2.7.4.22 11.3
-
 at 8.0 mM ATP, without GTP Neisseria meningitidis
2.7.4.22 11.8
-
 at 8.0 mM ATP, without GTP Haemophilus influenzae
2.7.4.22 12.8
-
 at 8.0 mM ATP, without GTP Staphylococcus aureus
2.7.4.22 18.1
-
 at 8.0 mM ATP, without GTP Bacillus subtilis
2.7.4.22 19.4
-
 at 8.0 mM ATP, with 0.5 mM GTP Enterococcus faecalis
2.7.4.22 23.5
-
 at 2.0 mM ATP, with 0.5 mM GTP Staphylococcus aureus
2.7.4.22 23.6
-
 at 8.0 mM ATP, with 0.5 mM GTP Staphylococcus aureus
2.7.4.22 26.1
-
 at 2.0 mM ATP, without GTP Escherichia coli
2.7.4.22 28.1
-
 at 8.0 mM ATP, without GTP Escherichia coli
2.7.4.22 28.6
-
 at 2.0 mM ATP, with 0.5 mM GTP Bacillus subtilis
2.7.4.22 38.9
-
 at 8.0 mM ATP, without GTP Streptococcus pneumoniae
2.7.4.22 39.1
-
 at 8.0 mM ATP, with 0.5 mM GTP Bacillus subtilis
2.7.4.22 46.8
-
 activity independent of concentrations of ATP, at 2.0 mM ATP, without GTP Salmonella enterica subsp. enterica serovar Typhimurium
2.7.4.22 47.5
-
 at 8.0 mM ATP, without GTP Salmonella enterica subsp. enterica serovar Typhimurium
2.7.4.22 52.8
-
 at 2.0 mM ATP, with 0.5 mM GTP Haemophilus influenzae
2.7.4.22 57.7
-
 at 2.0 mM ATP, with 0.5 mM GTP Neisseria meningitidis
2.7.4.22 60.5
-
 at 8.0 mM ATP, with 0.5 mM GTP Haemophilus influenzae
2.7.4.22 65.9
-
 at 2.0 mM ATP, with 0.5 mM GTP Escherichia coli
2.7.4.22 68.5
-
 at 2.0 mM ATP, with 0.5 mM GTP Streptococcus pneumoniae
2.7.4.22 71.1
-
 at 8.0 mM ATP, with 0.5 mM GTP Escherichia coli
2.7.4.22 87.4
-
 at 8.0 mM ATP, with 0.5 mM GTP Streptococcus pneumoniae
2.7.4.22 124.2
-
 at 8.0 mM ATP, with 0.5 mM GTP Salmonella enterica subsp. enterica serovar Typhimurium
2.7.4.22 134.3
-
 at 2.0 mM ATP, with 0.5 mM GTP Salmonella enterica subsp. enterica serovar Typhimurium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.4.22 ATP + UMP
-
 Salmonella enterica subsp. enterica serovar Typhimurium ADP + UDP
-
 r
2.7.4.22 ATP + UMP
-
 Staphylococcus aureus ADP + UDP
-
 r
2.7.4.22 ATP + UMP
-
 Haemophilus influenzae ADP + UDP
-
 r
2.7.4.22 ATP + UMP
-
 Enterococcus faecalis ADP + UDP
-
 r
2.7.4.22 ATP + UMP
-
 Streptococcus pneumoniae ADP + UDP
-
 r
2.7.4.22 ATP + UMP
-
 Bacillus subtilis ADP + UDP
-
 r
2.7.4.22 ATP + UMP
-
 Escherichia coli ADP + UDP
-
 r
2.7.4.22 ATP + UMP
-
 Neisseria meningitidis ADP + UDP
-
 r

Subunits

EC Number Subunits Comment Organism
2.7.4.22 hexamer gel filtration Staphylococcus aureus
2.7.4.22 hexamer gel filtration Enterococcus faecalis
2.7.4.22 hexamer gel filtration Streptococcus pneumoniae
2.7.4.22 hexamer gel filtration Bacillus subtilis
2.7.4.22 hexamer gel filtration Neisseria meningitidis
2.7.4.22 hexamer gel permeation Salmonella enterica subsp. enterica serovar Typhimurium
2.7.4.22 hexamer gel permeation Haemophilus influenzae
2.7.4.22 hexamer gel permeation Escherichia coli

Synonyms

EC Number Synonyms Comment Organism
2.7.4.22 ATP:UMP phosphotransferase
-
 Salmonella enterica subsp. enterica serovar Typhimurium
2.7.4.22 ATP:UMP phosphotransferase
-
 Staphylococcus aureus
2.7.4.22 ATP:UMP phosphotransferase
-
 Haemophilus influenzae
2.7.4.22 ATP:UMP phosphotransferase
-
 Enterococcus faecalis
2.7.4.22 ATP:UMP phosphotransferase
-
 Streptococcus pneumoniae
2.7.4.22 ATP:UMP phosphotransferase
-
 Bacillus subtilis
2.7.4.22 ATP:UMP phosphotransferase
-
 Escherichia coli
2.7.4.22 ATP:UMP phosphotransferase
-
 Neisseria meningitidis
2.7.4.22 UMP kinase
-
 Salmonella enterica subsp. enterica serovar Typhimurium
2.7.4.22 UMP kinase
-
 Staphylococcus aureus
2.7.4.22 UMP kinase
-
 Haemophilus influenzae
2.7.4.22 UMP kinase
-
 Enterococcus faecalis
2.7.4.22 UMP kinase
-
 Streptococcus pneumoniae
2.7.4.22 UMP kinase
-
 Bacillus subtilis
2.7.4.22 UMP kinase
-
 Escherichia coli
2.7.4.22 UMP kinase
-
 Neisseria meningitidis
2.7.4.22 uridine monophosphate kinase
-
 Salmonella enterica subsp. enterica serovar Typhimurium
2.7.4.22 uridine monophosphate kinase
-
 Staphylococcus aureus
2.7.4.22 uridine monophosphate kinase
-
 Haemophilus influenzae
2.7.4.22 uridine monophosphate kinase
-
 Enterococcus faecalis
2.7.4.22 uridine monophosphate kinase
-
 Streptococcus pneumoniae
2.7.4.22 uridine monophosphate kinase
-
 Bacillus subtilis
2.7.4.22 uridine monophosphate kinase
-
 Neisseria meningitidis
2.7.4.22 uridylate kinase
-
 Salmonella enterica subsp. enterica serovar Typhimurium
2.7.4.22 uridylate kinase
-
 Staphylococcus aureus
2.7.4.22 uridylate kinase
-
 Haemophilus influenzae
2.7.4.22 uridylate kinase
-
 Enterococcus faecalis
2.7.4.22 uridylate kinase
-
 Streptococcus pneumoniae
2.7.4.22 uridylate kinase
-
 Bacillus subtilis
2.7.4.22 uridylate kinase
-
 Neisseria meningitidis

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.4.22 0.11
-
 UMP at 2.0 mM ATP, without GTP Neisseria meningitidis
2.7.4.22 0.17
-
 UMP at 12 mM ATP, without GTP Haemophilus influenzae
2.7.4.22 0.23
-
 UMP at 10 mM ATP, without GTP Neisseria meningitidis
2.7.4.22 0.31
-
 UMP at 1.0 mM ATP, without GTP Haemophilus influenzae
2.7.4.22 0.44
-
 UMP at 2.0 mM ATP, without GTP Escherichia coli
2.7.4.22 0.6
-
 UMP at 2.0 mM ATP, without GTP Escherichia coli
2.7.4.22 0.66
-
 UMP at 2.0 mM ATP, without GTP, no inhibition at 30 mM ATP Bacillus subtilis
2.7.4.22 4.4
-
 UMP at 2.0 mM ATP, without GTP Streptococcus pneumoniae

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
2.7.4.22 0.01
-
 at 0.05 mM UMP, UMP protects for inhibition by Mg2+ free UTP Neisseria meningitidis UTP
2.7.4.22 0.13
-
 at 0.2 mM UMP, UMP protects for inhibition by Mg2+ free UTP Neisseria meningitidis UTP