EC Number | Cloned (Comment) | Organism |
---|---|---|
2.4.1.170 | expressed in Escherichia coli BL21(DE3) | Glycine max |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.4.1.170 | D125A | in the mutant the kcat is lower and the Km is higher than in wild type enzyme | Glycine max |
2.4.1.170 | E376A | in the mutant the kcat is lower and the Km is higher than in wild type enzyme | Glycine max |
2.4.1.170 | E392A | in the mutant the kcat is lower and the Km is higher than in wild type enzyme | Glycine max |
2.4.1.170 | E392D | in the mutant the kcat is higher and the Km is lower than in wild type enzyme | Glycine max |
2.4.1.170 | E456A | in the mutant the kcat is lower and the Km is slightly lower than in wild type enzyme | Glycine max |
2.4.1.170 | H15A | in the mutant the kcat is lower and the Km is higher than in wild type enzyme | Glycine max |
2.4.1.170 | H359A | in the mutant the kcat is lower and the Km has the same level as in wild type enzyme | Glycine max |
2.4.1.170 | H368A | in the mutant the kcat is higher and the Km is higher than in wild type enzyme | Glycine max |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.4.1.170 | Fe2+ | 0.1 mM, residual activity 28% | Glycine max | |
2.4.1.170 | Hg2+ | 0.1 mM, residual activity 26% | Glycine max | |
2.4.1.170 | additional information | 1 mM EDTA, 1mM uridine, 1 mM UMP, 1 mM UDP, 1 mM UTP, 1 mM glucose, 1 mM diethylpyrocarbonatem or 1 mM phenylmethanesulfonyl fluoride have negligibel effect on the catalytic activity (residual activity, in excess of 64%). | Glycine max |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.4.1.170 | 0.00077 | - |
genistein | E392D mutant, enzyme assay is run at pH 8.5 at 30°C | Glycine max | |
2.4.1.170 | 0.0026 | - |
genistein | E456A mutant, enzyme assay is run at pH 8.5 at 30°C | Glycine max | |
2.4.1.170 | 0.0027 | - |
genistein | H359A muant, enzyme assay is run at pH 8.5 at 30°C | Glycine max | |
2.4.1.170 | 0.0027 | - |
genistein | wild-type, enzyme assay is run at pH 8.5 at 30°C | Glycine max | |
2.4.1.170 | 0.0036 | - |
genistein | purified enzyme, enzyme assay is run at pH 8.5 at 30°C | Glycine max | |
2.4.1.170 | 0.0045 | - |
genistein | E376A mutant, enzyme assay is run at pH 8.5 at 30°C | Glycine max | |
2.4.1.170 | 0.0049 | - |
genistein | H368A mutant, enzyme assay is run at pH 8.5 at 30°C | Glycine max | |
2.4.1.170 | 0.011 | - |
genistein | E392A mutant, enzyme assay is run at pH 8.5 at 30°C | Glycine max | |
2.4.1.170 | 0.016 | - |
genistein | H15A mutant, enzyme assay is run at pH 8.5 at 30°C | Glycine max | |
2.4.1.170 | 0.024 | - |
UDP-glucose | E392D mutant, enzyme assay is run at pH 8.5 at 30°C | Glycine max | |
2.4.1.170 | 0.027 | - |
UDP-glucose | H15A mutant, enzyme assay is run at pH 8.5 at 30°C | Glycine max | |
2.4.1.170 | 0.045 | - |
UDP-glucose | D125A mutant, enzyme assay is run at pH 8.5 at 30°C | Glycine max | |
2.4.1.170 | 0.06 | - |
genistein | D125A mutant, enzyme assay is run at pH 8.5 at 30°C | Glycine max | |
2.4.1.170 | 0.072 | - |
UDP-glucose | H359A muant, enzyme assay is run at pH 8.5 at 30°C | Glycine max | |
2.4.1.170 | 0.081 | - |
UDP-glucose | H368A mutant, enzyme assay is run at pH 8.5 at 30°C | Glycine max | |
2.4.1.170 | 0.082 | - |
UDP-glucose | E456A mutant, enzyme assay is run at pH 8.5 at 30°C | Glycine max | |
2.4.1.170 | 0.13 | - |
UDP-glucose | wild-type, enzyme assay is run at pH 8.5 at 30°C | Glycine max | |
2.4.1.170 | 0.19 | - |
UDP-glucose | E392A mutant, enzyme assay is run at pH 8.5 at 30°C | Glycine max | |
2.4.1.170 | 0.19 | - |
UDP-glucose | purified enzyme, enzyme assay is run at pH 8.5 at 30°C | Glycine max | |
2.4.1.170 | 2.1 | - |
UDP-glucose | E376A mutant, enzyme assay is run at pH 8.5 at 30°C | Glycine max |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.4.1.170 | additional information | Ca2+, Cd2+, Co2+, Cu2+, Mg2+, Mn2+, Ni2+, Sn2+ or Zn2+ (all at 0.1 mM) have negligible effect on the catalytic activity. | Glycine max |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.4.1.170 | 45000 | - |
SDS-PAGE | Glycine max |
2.4.1.170 | 46000 | - |
gel filtration chromatography | Glycine max |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.4.1.170 | Glycine max | - |
soybean, Merr. cv. Wase-Hakucho | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.4.1.170 | DEAE-Sepharose Fast Flow column, Q-Sepharose HP column, Phenyl-Sepharose HP column (pH 8.5), hydroxyapatite column, gel filtration (HiLoad 26/60 Superdex 200pg column), resource Q column and chromatofocusing on Mono P (Mono P column), all at 4°C. The activity in the crude extract is extremely unstable to oxidation and the addition of 2-mercaptoethanol is essential for its efficient purification. The addition of CHAPS to purification buffers is also essential for purification after step 6 (hydroxyapatite chromatography), otherwise the activity is irreversibly lost. | Glycine max |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.4.1.170 | root | roots of 7-9 day-old seedlings | Glycine max | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.4.1.170 | additional information | - |
as glucosyl acceptor genistein shows the highest activity (100%), daidzein has 73%, formononetin shows 67%, quercetin 32%, kaempferol 19%, 4,2',4',6'-tetrahydoxychalcone 15%, apigenin 14%, aureusidin 5%, esculetin 4% and naringenin shows 3% activity (all tested with UDP-glucose). Enzyme assay is run at pH 8.5 at 30°C | Glycine max |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.1.170 | additional information | none of the following phenolics serve as glucosyl acceptors: cyanidin, trans-p-coumaric acid, caffeic acid, benzoic acid, m- and p-hydroybenzoic acid, salicylic acid, salicyl alcohol and hydroquinone. | Glycine max | ? | - |
? | |
2.4.1.170 | additional information | the relative activites for UDP-glucuronic acid and for UDP-galactose is 0% and for UDP-glucose 12%, all tested with genistein as glycosyl acceptor | Glycine max | ? | - |
? | |
2.4.1.170 | UDP-glucose + 4,2',4',6'-tetrahydroxychalcone | - |
Glycine max | UDP + ? | - |
? | |
2.4.1.170 | UDP-glucose + apigenin | - |
Glycine max | UDP + apigenin 7-O-beta-D-glucoside | - |
? | |
2.4.1.170 | UDP-glucose + aureusidin | - |
Glycine max | UDP + aureusidin 6-O-beta-D-glucoside | - |
? | |
2.4.1.170 | UDP-glucose + daidzein | - |
Glycine max | UDP + daidzein 7-O-beta-D-glucoside | - |
? | |
2.4.1.170 | UDP-glucose + esculetin | - |
Glycine max | UDP + esculetin 7-O-beta-D-glucoside | - |
? | |
2.4.1.170 | UDP-glucose + formononetin | - |
Glycine max | UDP + formononetin 7-O-beta-D-glucoside | - |
? | |
2.4.1.170 | UDP-glucose + genistein | - |
Glycine max | UDP + genistein 7-O-beta-D-glucoside | - |
? | |
2.4.1.170 | UDP-glucose + kaempferol | - |
Glycine max | UDP + kaempferol 7-O-beta-D-glucoside | - |
? | |
2.4.1.170 | UDP-glucose + naringenin | - |
Glycine max | UDP + naringenin 7-O-beta-D-glucoside | - |
? | |
2.4.1.170 | UDP-glucose + quercetin | - |
Glycine max | UDP + quercetin 7-O-beta-D-glucoside | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.4.1.170 | monomer | - |
Glycine max |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.4.1.170 | GmIF7GT | UDP-glucose:isoflavone 7-O-glucosyltransferase | Glycine max |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.4.1.170 | additional information | - |
below 30°C at pH 8.5 for 1h | Glycine max |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.4.1.170 | 0.0004 | - |
genistein | E392A mutant, enzyme assay is run at pH 8.5 at 30°C | Glycine max | |
2.4.1.170 | 0.13 | - |
genistein | H15A mutant, enzyme assay is run at pH 8.5 at 30°C | Glycine max | |
2.4.1.170 | 0.41 | - |
genistein | E456A mutant, enzyme assay is run at pH 8.5 at 30°C | Glycine max | |
2.4.1.170 | 0.59 | - |
genistein | H359A muant, enzyme assay is run at pH 8.5 at 30°C | Glycine max | |
2.4.1.170 | 0.63 | - |
genistein | E376A mutant, enzyme assay is run at pH 8.5 at 30°C | Glycine max | |
2.4.1.170 | 0.66 | - |
genistein | D125A mutant, enzyme assay is run at pH 8.5 at 30°C | Glycine max | |
2.4.1.170 | 0.74 | - |
genistein | purified enzyme, enzyme assay is run at pH 8.5 at 30°C | Glycine max | |
2.4.1.170 | 0.76 | - |
genistein | wild-type, enzyme assay is run at pH 8.5 at 30°C | Glycine max | |
2.4.1.170 | 2 | - |
genistein | H368A mutant, enzyme assay is run at pH 8.5 at 30°C | Glycine max | |
2.4.1.170 | 3 | - |
genistein | E392D mutant, enzyme assay is run at pH 8.5 at 30°C | Glycine max |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.4.1.170 | 8.5 | - |
optimal pH at 30°C | Glycine max |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
2.4.1.170 | 7 | 9 | active over this pH range | Glycine max |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
2.4.1.170 | 5.5 | 10 | at 20°C for 8 h | Glycine max |