EC Number | Application | Comment | Organism |
---|---|---|---|
1.3.7.5 | additional information | phycocyanobilin-CpcB(C155I) and phycocyanobilin-PecB(C155I) are also biosynthesized heterologously in vivo, when cpeS is introduced into Escherichia coli with cpcB(C155I) or pecB(C155I), respectively, together with genes ho1 and pcyA | Anabaena sp. |
EC Number | Cloned (Comment) | Organism |
---|---|---|
1.3.7.5 | coexpression in Escherichia coli BL21(DE3) with cpcB(C155I) or pecB(C155I) and with cpeS, ho1 and pcyA, resulting in biosynthesis of the respective beta-subunits singly chromophorylated at cysteine-beta84 | Anabaena sp. |
4.4.1.29 | overexpressed in Escherichia coli | Anabaena sp. |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
4.4.1.29 | Co2+ | 5 mM, complete inhibition | Anabaena sp. | |
4.4.1.29 | Cu2+ | 5 mM, complete inhibition | Anabaena sp. | |
4.4.1.29 | Fe2+ | 5 mM, 46% inhibition | Anabaena sp. | |
4.4.1.29 | Mn2+ | 5 mM, 12% inhibition | Anabaena sp. | |
4.4.1.29 | additional information | no effect up to 20 mM EDTA | Anabaena sp. | |
4.4.1.29 | Ni2+ | 5 mM, complete inhibition | Anabaena sp. | |
4.4.1.29 | Zn2+ | 5 mM, complete inhibition | Anabaena sp. |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.4.1.29 | 0.0023 | - |
(2R,3E)-phycocyanobilin | pH 7.5, 37°C, cosubstrate: apo-[phycoerythrocyanin beta-subunit] mutant C155I | Anabaena sp. | |
4.4.1.29 | 0.0027 | - |
(2R,3E)-phycocyanobilin | pH 7.5, 37°C, cosubstrate: apo-[C-phycocyanin beta-subunit] mutant C155I | Anabaena sp. |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
4.4.1.29 | NaCl | optimal concentration: 150 mM | Anabaena sp. |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.4.1.29 | apo-[C-phycocyanin beta-subunit] + (2R,3E)-phycocyanobilin | Anabaena sp. | the enzyme attaches phycobilin chromophores to cysteine 84 of several phycobiliproteins | [C-phycocyanin beta-subunit]-Cys84-phycocyanobilin | - |
? | |
4.4.1.29 | apo-[C-phycocyanin beta-subunit] + (2R,3E)-phycocyanobilin | Anabaena sp. PCC 7120 | the enzyme attaches phycobilin chromophores to cysteine 84 of several phycobiliproteins | [C-phycocyanin beta-subunit]-Cys84-phycocyanobilin | - |
? | |
4.4.1.29 | apo-[phycoerythrocyanin beta-subunit] + (2R,3E)-phycocyanobilin | Anabaena sp. | the enzyme attaches phycobilin chromophores to cysteine 84 of several phycobiliproteins | [phycoerythrocyanin beta-subunit]-Cys84-phycocyanobilin | - |
? | |
4.4.1.29 | apo-[phycoerythrocyanin beta-subunit] + (2R,3E)-phycocyanobilin | Anabaena sp. PCC 7120 | the enzyme attaches phycobilin chromophores to cysteine 84 of several phycobiliproteins | [phycoerythrocyanin beta-subunit]-Cys84-phycocyanobilin | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.3.7.5 | Anabaena sp. | - |
- |
- |
1.3.7.5 | Anabaena sp. PCC 7120 | - |
- |
- |
4.4.1.29 | Anabaena sp. | Q8YZ70 | - |
- |
4.4.1.29 | Anabaena sp. PCC 7120 | Q8YZ70 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
4.4.1.29 | - |
Anabaena sp. |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
4.4.1.29 | [allophycocyanin alpha-subunit]-Cys84-phycocyanobilin = apo-[allophycocyanin alpha-subunit] + (2R,3E)-phycocyanobilin | (3) | Anabaena sp. | |
4.4.1.29 | [allophycocyanin beta-subunit]-Cys84-phycocyanobilin = apo-[allophycocyanin beta-subunit] + (2R,3E)-phycocyanobilin | (4) | Anabaena sp. | |
4.4.1.29 | [C-phycocyanin beta-subunit]-Cys84-phycocyanobilin = apo-[C-phycocyanin beta-subunit] + (2R,3E)-phycocyanobilin | (1) | Anabaena sp. | |
4.4.1.29 | [C-phycoerythrin alpha-subunit]-Cys84-phycoerythrobilin = apo-[C-phycoerythrin alpha-subunit] + (2R,3E)-phycoerythrobilin | (5) | Anabaena sp. | |
4.4.1.29 | [C-phycoerythrin beta-subunit]-Cys84-phycoerythrobilin = apo-[C-phycoerythrin beta-subunit] + (2R,3E)-phycoerythrobilin | (6) | Anabaena sp. | |
4.4.1.29 | [phycoerythrocyanin beta-subunit]-Cys84-phycocyanobilin = apo-[phycoerythrocyanin beta-subunit] + (2R,3E)-phycocyanobilin | (2) | Anabaena sp. |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.3.7.5 | biliverdin IXa + reduced ferredoxin | - |
Anabaena sp. | (3Z)-phycocyanobilin + oxidized ferredoxin | - |
? | |
1.3.7.5 | biliverdin IXa + reduced ferredoxin | - |
Anabaena sp. PCC 7120 | (3Z)-phycocyanobilin + oxidized ferredoxin | - |
? | |
4.4.1.29 | apo-[C-phycocyanin beta-subunit] + (2R,3E)-phycocyanobilin | the enzyme attaches phycobilin chromophores to cysteine 84 of several phycobiliproteins | Anabaena sp. | [C-phycocyanin beta-subunit]-Cys84-phycocyanobilin | - |
? | |
4.4.1.29 | apo-[C-phycocyanin beta-subunit] + (2R,3E)-phycocyanobilin | the enzyme attaches (2R,3E)-phycocyanobilin to native apo C-phycocyanin beta-subunit and to the C155I-mutant, but not to theC84S mutant | Anabaena sp. | [C-phycocyanin beta-subunit]-Cys84-phycocyanobilin | - |
? | |
4.4.1.29 | apo-[C-phycocyanin beta-subunit] + (2R,3E)-phycocyanobilin | the enzyme attaches phycobilin chromophores to cysteine 84 of several phycobiliproteins | Anabaena sp. PCC 7120 | [C-phycocyanin beta-subunit]-Cys84-phycocyanobilin | - |
? | |
4.4.1.29 | apo-[C-phycocyanin beta-subunit] + (2R,3E)-phycocyanobilin | the enzyme attaches (2R,3E)-phycocyanobilin to native apo C-phycocyanin beta-subunit and to the C155I-mutant, but not to theC84S mutant | Anabaena sp. PCC 7120 | [C-phycocyanin beta-subunit]-Cys84-phycocyanobilin | - |
? | |
4.4.1.29 | apo-[phycoerythrocyanin beta-subunit] + (2R,3E)-phycocyanobilin | the enzyme attaches phycobilin chromophores to cysteine 84 of several phycobiliproteins | Anabaena sp. | [phycoerythrocyanin beta-subunit]-Cys84-phycocyanobilin | - |
? | |
4.4.1.29 | apo-[phycoerythrocyanin beta-subunit] + (2R,3E)-phycocyanobilin | the enzyme attaches (2R,3E)-phycocyanobilin to native apo phycoerythrocyanin beta-subunit and to the C155I-mutant, but not to theC84S mutant | Anabaena sp. | [phycoerythrocyanin beta-subunit]-Cys84-phycocyanobilin | - |
? | |
4.4.1.29 | apo-[phycoerythrocyanin beta-subunit] + (2R,3E)-phycocyanobilin | the enzyme attaches phycobilin chromophores to cysteine 84 of several phycobiliproteins | Anabaena sp. PCC 7120 | [phycoerythrocyanin beta-subunit]-Cys84-phycocyanobilin | - |
? | |
4.4.1.29 | apo-[phycoerythrocyanin beta-subunit] + (2R,3E)-phycocyanobilin | the enzyme attaches (2R,3E)-phycocyanobilin to native apo phycoerythrocyanin beta-subunit and to the C155I-mutant, but not to theC84S mutant | Anabaena sp. PCC 7120 | [phycoerythrocyanin beta-subunit]-Cys84-phycocyanobilin | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.3.7.5 | PcyA | - |
Anabaena sp. |
4.4.1.29 | alr0617 | - |
Anabaena sp. |
4.4.1.29 | cpeS | - |
Anabaena sp. |
4.4.1.29 | phycocyanobilin:Cys-beta84-phycobiliprotein lyase | - |
Anabaena sp. |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
4.4.1.29 | 40 | - |
substrate: apo-[phycoerythrocyanin beta-subunit] mutant C155 | Anabaena sp. |
4.4.1.29 | 45 | - |
substrate: apo-[C-phycocyanin beta-subunit] | Anabaena sp. |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
4.4.1.29 | 30 | 55 | 30°C: about 55% of maximal activity, 55°C: about 70% of maximal activity, substrate: apo-[C-phycocyanin beta-subunit] | Anabaena sp. |
4.4.1.29 | 30 | 55 | 30°C: about 75% of maximal activity, 55°C: about 50% of maximal activity, substrate: apo-[phycoerythrocyanin beta-subunit] mutant C155 | Anabaena sp. |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.4.1.29 | 0.000011 | - |
(2R,3E)-phycocyanobilin | pH 7.5, 37°C, cosubstrate: apo phycoerythrocyanin beta-subunit mutant C155I | Anabaena sp. | |
4.4.1.29 | 0.000017 | - |
(2R,3E)-phycocyanobilin | pH 7.5, 37°C, cosubstrate: apo C-phycocyanin beta-subunit mutant C155I | Anabaena sp. |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
4.4.1.29 | 7 | 9 | substrate: apo-[C-phycocyanin beta-subunit] | Anabaena sp. |
4.4.1.29 | 7.5 | 8 | substrate: apo-[phycoerythrocyanin beta-subunit] mutant C155 | Anabaena sp. |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
4.4.1.29 | 6.5 | 9.5 | pH 6.5: about 50% of maximal activity, pH 9.5: about 75% of maximal activity, substrate: apo-[C-phycocyanin beta-subunit] | Anabaena sp. |
4.4.1.29 | 7 | 8.5 | pH 7.0: about 80% of maximal activity, pH 7.0: about 80% of maximal activity, substrate: apo-[phycoerythrocyanin beta-subunit] mutant C155 | Anabaena sp. |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.4.1.29 | 0.0048 | - |
(2R,3E)-phycocyanobilin | pH 7.5, 37°C, cosubstrate: apo-[phycoerythrocyanin beta-subunit] mutant C155I | Anabaena sp. | |
4.4.1.29 | 0.0063 | - |
(2R,3E)-phycocyanobilin | pH 7.5, 37°C, cosubstrate: apo-[C-phycocyanin beta-subunit] mutant C155I | Anabaena sp. |