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Literature summary extracted from

  • Ferella, M.; Montalvetti, A.; Rohloff, P.; Miranda, K.; Fang, J.; Reina, S.; Kawamukai, M.; Bua, J.; Nilsson, D.; Pravia, C.; Katzin, A.; Cassera, M.B.; Aslund, L.; Andersson, B.; Docampo, R.; Bontempi, E.J.
    A solanesyl-diphosphate synthase localizes in glycosomes of Trypanosoma cruzi (2006), J. Biol. Chem., 281, 39339-39348.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.85 expression of TcSPPS is able to complement an Escherichia coli ispB mutant Trypanosoma cruzi

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.85 0.00459
-
 farnesyl diphosphate pH 7.4, 37°C Trypanosoma cruzi
2.5.1.85 0.00707
-
 geranylgeranyl diphosphate pH 7.4, 37°C Trypanosoma cruzi
2.5.1.85 0.0192
-
 isopentenyl diphosphate pH 7.4, 37°C, cosubstrate: geranyl diphosphate Trypanosoma cruzi
2.5.1.85 0.0243
-
 isopentenyl diphosphate pH 7.4, 37°C, cosubstrate: geranylgeranyl diphosphate Trypanosoma cruzi
2.5.1.85 0.0307
-
 isopentenyl diphosphate pH 7.4, 37°C, cosubstrate: farnesyl diphosphate Trypanosoma cruzi
2.5.1.85 0.0548
-
 geranyl diphosphate pH 7.4, 37°C Trypanosoma cruzi

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.5.1.85 glycosome
-
 Trypanosoma cruzi 20015
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.5.1.85 Mg2+ optimal level of activity obtained at 0.5-1 mM. No activation by Mn2+ Trypanosoma cruzi

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.85 39000
-
 x * 39000, calculated from sequence Trypanosoma cruzi

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.85 geranylgeranyl diphosphate + 5 isopentenyl diphosphate Trypanosoma cruzi the enzyme is involved in synthesis of ubiquinone-9 5 diphosphate + all-trans-nonaprenyl diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.85 Trypanosoma cruzi Q964Q8
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.85
-
 Trypanosoma cruzi

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.5.1.85 epimastigote
-
 Trypanosoma cruzi
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.85 (2E,6E)-farnesyl diphosphate + 6 isopentenyl diphosphate
-
 Trypanosoma cruzi 6 diphosphate + all-trans-nonaprenyl diphosphate
-
 ?
2.5.1.85 dimethylallyl diphosphate + 8 isopentenyl diphosphate low activity Trypanosoma cruzi 8 diphosphate + all-trans-nonaprenyl diphosphate
-
 ?
2.5.1.85 geranyl diphosphate + 7 isopentenyl diphosphate
-
 Trypanosoma cruzi 7 diphosphate + all-trans-nonaprenyl diphosphate
-
 ?
2.5.1.85 geranylgeranyl diphosphate + 5 isopentenyl diphosphate geranylgeranyl diphosphate is the preferred allylic substrate Trypanosoma cruzi 5 diphosphate + all-trans-nonaprenyl diphosphate
-
 ?
2.5.1.85 geranylgeranyl diphosphate + 5 isopentenyl diphosphate the enzyme is involved in synthesis of ubiquinone-9 Trypanosoma cruzi 5 diphosphate + all-trans-nonaprenyl diphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.85 ? x * 39000, calculated from sequence Trypanosoma cruzi

Synonyms

EC Number Synonyms Comment Organism
2.5.1.85 solanesyl-diphosphate synthase
-
 Trypanosoma cruzi
2.5.1.85 SPPS
-
 Trypanosoma cruzi
2.5.1.85 TCSPPS
-
 Trypanosoma cruzi

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.5.1.85 0.032
-
 (2E,6E)-farnesyl diphosphate pH 7.4, 37°C Trypanosoma cruzi
2.5.1.85 0.043
-
 isopentenyl diphosphate pH 7.4, 37°C, cosubstrate: farnesyl diphosphate Trypanosoma cruzi
2.5.1.85 0.065
-
 geranyl diphosphate pH 7.4, 37°C Trypanosoma cruzi
2.5.1.85 0.087
-
 geranylgeranyl diphosphate pH 7.4, 37°C Trypanosoma cruzi
2.5.1.85 0.131
-
 isopentenyl diphosphate pH 7.4, 37°C, cosubstrate: geranyl diphosphate Trypanosoma cruzi
2.5.1.85 0.217
-
 isopentenyl diphosphate pH 7.4, 37°C, cosubstrate: geranylgeranyl diphosphate Trypanosoma cruzi

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.5.1.85 Trypanosoma cruzi calculated from sequence
-
 6.01

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.5.1.85 0.00119
-
 geranyl diphosphate pH 7.4, 37°C Trypanosoma cruzi
2.5.1.85 0.0014
-
 isopentenyl diphosphate pH 7.4, 37°C, cosubstrate: farnesyl diphosphate Trypanosoma cruzi
2.5.1.85 0.00685
-
 isopentenyl diphosphate pH 7.4, 37°C, cosubstrate: geranyl diphosphate Trypanosoma cruzi
2.5.1.85 0.00701
-
 (2E,6E)-farnesyl diphosphate pH 7.4, 37°C Trypanosoma cruzi
2.5.1.85 0.00893
-
 isopentenyl diphosphate pH 7.4, 37°C, cosubstrate: geranylgeranyl diphosphate Trypanosoma cruzi
2.5.1.85 0.012
-
 geranylgeranyl diphosphate pH 7.4, 37°C Trypanosoma cruzi