EC Number | Cloned (Comment) | Organism |
---|---|---|
6.3.2.1 | expression in Escherichia coli as N-terminal His-tagged protein | Arabidopsis thaliana |
6.3.2.1 | expression in Escherichia coli BL21 as N-terminal His-tagged protein | Escherichia coli |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
6.3.2.1 | E132A | kcat for beta-alanine is 3.7fold higher than wild-type value, kcat for pantoate is 21fold higher than wild-type value | Arabidopsis thaliana |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
6.3.2.1 | (R)-pantoate | substrate inhibition | Arabidopsis thaliana |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
6.3.2.1 | ATP + (R)-pantoate + beta-alanine | Escherichia coli | synthesis of pantothenate, the essential precursor to coenzyme A | AMP + diphosphate + (R)-pantothenate | - |
? | |
6.3.2.1 | ATP + (R)-pantoate + beta-alanine | Arabidopsis thaliana | synthesis of pantothenate, the essential precursor to coenzyme A | AMP + diphosphate + (R)-pantothenate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
6.3.2.1 | Arabidopsis thaliana | - |
- |
- |
6.3.2.1 | Escherichia coli | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
6.3.2.1 | - |
Escherichia coli |
6.3.2.1 | - |
Arabidopsis thaliana |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
6.3.2.1 | ATP + (R)-pantoate + beta-alanine | synthesis of pantothenate, the essential precursor to coenzyme A | Escherichia coli | AMP + diphosphate + (R)-pantothenate | - |
? | |
6.3.2.1 | ATP + (R)-pantoate + beta-alanine | synthesis of pantothenate, the essential precursor to coenzyme A | Arabidopsis thaliana | AMP + diphosphate + (R)-pantothenate | - |
? | |
6.3.2.1 | ATP + (R)-pantoate + beta-alanine | Bi Uni Uni Bi kinetic mechanism. Enzyme displays non-allosteric behavior | Escherichia coli | AMP + diphosphate + (R)-pantothenate | - |
? | |
6.3.2.1 | ATP + (R)-pantoate + beta-alanine | the two-step reaction mechanism, large allosteric effects | Arabidopsis thaliana | AMP + diphosphate + (R)-pantothenate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
6.3.2.1 | dimer | - |
Escherichia coli |
6.3.2.1 | dimer | - |
Arabidopsis thaliana |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
6.3.2.1 | 0.039 | - |
(R)-pantoate | 25°C, pH 8.0, wild-type enzyme | Arabidopsis thaliana | |
6.3.2.1 | 0.16 | - |
beta-Alanine | 25°C, pH 8.0, wild-type enzyme | Arabidopsis thaliana | |
6.3.2.1 | 0.59 | - |
beta-Alanine | 25°C, pH 8.0, mutant enzyme E132A | Arabidopsis thaliana | |
6.3.2.1 | 0.81 | - |
(R)-pantoate | 25°C, pH 8.0, mutant enzyme E132A | Arabidopsis thaliana |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
6.3.2.1 | 5.5 | - |
exchange between beta-alanine and pantothenate in the presence of AMP | Escherichia coli |
6.3.2.1 | 5.5 | - |
exchange between beta-alanine and pantothenate in the presence of AMP | Arabidopsis thaliana |
6.3.2.1 | 9 | - |
synthesis of pantothenate | Escherichia coli |
6.3.2.1 | 9 | - |
synthesis of pantothenate | Arabidopsis thaliana |