EC Number | Application | Comment | Organism |
---|---|---|---|
1.3.7.5 | additional information | conserved histidine and aspartate residues essential for the catalytic activity of the enzyme, direct role of the His85-Asp102 pair in exovinyl reduction of biliverdin IXa | Nostoc sp. |
EC Number | Cloned (Comment) | Organism |
---|---|---|
1.3.7.5 | wild-type and mutants, mutant plasmid constructs expressed in Escherichia coli strain DH5alpha | Nostoc sp. |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.3.7.5 | C210A | retains 90% relative to wild type | Nostoc sp. |
1.3.7.5 | C86A | retains 90% activity relative to wild type | Nostoc sp. |
1.3.7.5 | D102N | retains 11% activity relative to wild type | Nostoc sp. |
1.3.7.5 | D116N | retains 11% activity relative to wild type | Nostoc sp. |
1.3.7.5 | D217N | retains 87% relative to wild type | Nostoc sp. |
1.3.7.5 | H71A | retains less than 1% activity relative to wild type | Nostoc sp. |
1.3.7.5 | H71D | retains 28% activity relative to wild type | Nostoc sp. |
1.3.7.5 | H71E | retains 50% activity relative to wild type | Nostoc sp. |
1.3.7.5 | H71N | retains 46% activity relative to wild type | Nostoc sp. |
1.3.7.5 | H71Q | retains 65% activity relative to wild type | Nostoc sp. |
1.3.7.5 | H85A | retains less than 1% activity relative to wild type | Nostoc sp. |
1.3.7.5 | H85D | retains less than 1% activity relative to wild type | Nostoc sp. |
1.3.7.5 | H85E | retains 5% activity relative to wild type | Nostoc sp. |
1.3.7.5 | H85N | retains less than 1% activity relative to wild type | Nostoc sp. |
1.3.7.5 | H85Q | retains 5% activity relative to wild type | Nostoc sp. |
1.3.7.5 | K218E | retains 20% activity relative to wild type | Nostoc sp. |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.3.7.5 | diethylpyrocarbonate | reduces PcyA activity to less than 20% of mock-treated enzyme within 10 min, diethylpyrocarbonate-inactivation can be prevented by the presence of biliverdin IXa substrate | Nostoc sp. |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.3.7.5 | Nostoc sp. | - |
- |
- |
1.3.7.5 | Nostoc sp. PCC 7120 | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.3.7.5 | wild-type and mutants, histidine mutants purified to homogeneity | Nostoc sp. |
EC Number | Storage Stability | Organism |
---|---|---|
1.3.7.5 | -80°C | Nostoc sp. |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.3.7.5 | biliverdin IXa + reduced ferredoxin | - |
Nostoc sp. | (3Z)-phycocyanobilin + oxidized ferredoxin | - |
? | |
1.3.7.5 | biliverdin IXa + reduced ferredoxin | - |
Nostoc sp. PCC 7120 | (3Z)-phycocyanobilin + oxidized ferredoxin | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.3.7.5 | PcyA | - |
Nostoc sp. |