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Literature summary extracted from

  • Tu, S.L.; Sughrue, W.; Britt, R.D.; Lagarias, J.C.
    A conserved histidine-aspartate pair is required for exovinyl reduction of biliverdin by a cyanobacterial phycocyanobilin:ferredoxin oxidoreductase (2006), J. Biol. Chem., 281, 3127-3136.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.3.7.5 additional information conserved histidine and aspartate residues essential for the catalytic activity of the enzyme, direct role of the His85-Asp102 pair in exovinyl reduction of biliverdin IXa Nostoc sp.

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.7.5 wild-type and mutants, mutant plasmid constructs expressed in Escherichia coli strain DH5alpha Nostoc sp.

Protein Variants

EC Number Protein Variants Comment Organism
1.3.7.5 C210A retains 90% relative to wild type Nostoc sp.
1.3.7.5 C86A retains 90% activity relative to wild type Nostoc sp.
1.3.7.5 D102N retains 11% activity relative to wild type Nostoc sp.
1.3.7.5 D116N retains 11% activity relative to wild type Nostoc sp.
1.3.7.5 D217N retains 87% relative to wild type Nostoc sp.
1.3.7.5 H71A retains less than 1% activity relative to wild type Nostoc sp.
1.3.7.5 H71D retains 28% activity relative to wild type Nostoc sp.
1.3.7.5 H71E retains 50% activity relative to wild type Nostoc sp.
1.3.7.5 H71N retains 46% activity relative to wild type Nostoc sp.
1.3.7.5 H71Q retains 65% activity relative to wild type Nostoc sp.
1.3.7.5 H85A retains less than 1% activity relative to wild type Nostoc sp.
1.3.7.5 H85D retains less than 1% activity relative to wild type Nostoc sp.
1.3.7.5 H85E retains 5% activity relative to wild type Nostoc sp.
1.3.7.5 H85N retains less than 1% activity relative to wild type Nostoc sp.
1.3.7.5 H85Q retains 5% activity relative to wild type Nostoc sp.
1.3.7.5 K218E retains 20% activity relative to wild type Nostoc sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.3.7.5 diethylpyrocarbonate reduces PcyA activity to less than 20% of mock-treated enzyme within 10 min, diethylpyrocarbonate-inactivation can be prevented by the presence of biliverdin IXa substrate Nostoc sp.

Organism

EC Number Organism UniProt Comment Textmining
1.3.7.5 Nostoc sp.
-
-
-
1.3.7.5 Nostoc sp. PCC 7120
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.7.5 wild-type and mutants, histidine mutants purified to homogeneity Nostoc sp.

Storage Stability

EC Number Storage Stability Organism
1.3.7.5 -80°C Nostoc sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.7.5 biliverdin IXa + reduced ferredoxin
-
Nostoc sp. (3Z)-phycocyanobilin + oxidized ferredoxin
-
?
1.3.7.5 biliverdin IXa + reduced ferredoxin
-
Nostoc sp. PCC 7120 (3Z)-phycocyanobilin + oxidized ferredoxin
-
?

Synonyms

EC Number Synonyms Comment Organism
1.3.7.5 PcyA
-
Nostoc sp.