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Literature summary extracted from

  • Saitoh, T.; Ikegami, T.; Nakayama, M.; Teshima, K.; Akutsu, H.; Hase, T.
    NMR study of the electron transfer complex of plant ferredoxin and sulfite reductase: mapping the interaction sites of ferredoxin (2006), J. Biol. Chem., 281, 10482-10488.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.8.7.1 hanging drop vapour diffusion method Zea mays

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.8.7.1 0.004
-
reduced ferredoxin using wild type ferredoxin, the reaction mixture contains ferredoxin-NADP+ reductase (100 nM), sulfite reductase (ferredoxin) (800 nM), and sulfite (0.5 mM) in 30 mM potassium phosphate buffer, pH 7.0 and 25 mM NaCl Zea mays
1.8.7.1 0.0062
-
reduced ferredoxin using E92Q/E93Q mutant ferredoxin, the reaction mixture contains ferredoxin-NADP+ reductase (100 nM), sulfite reductase (ferredoxin) (800 nM), and sulfite (0.5 mM) in 30 mM potassium phosphate buffer, pH 7.0 and 25 mM NaCl Zea mays
1.8.7.1 0.0095
-
reduced ferredoxin using E92Q/E30Q/D34N mutant ferredoxin, the reaction mixture contains ferredoxin-NADP+ reductase (100 nM), sulfite reductase (ferredoxin) (800 nM), and sulfite (0.5 mM) in 30 mM potassium phosphate buffer, pH 7.0 and 25 mM NaCl Zea mays
1.8.7.1 0.0147
-
reduced ferredoxin using E92Q/E93Q/E29Q/D34N mutant ferredoxin, the reaction mixture contains ferredoxin-NADP+ reductase (100 nM), sulfite reductase (ferredoxin) (800 nM), and sulfite (0.5 mM) in 30 mM potassium phosphate buffer, pH 7.0 and 25 mM NaCl Zea mays

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.8.7.1 4Fe-4S
-
Zea mays

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.8.7.1 65000
-
1 * 65000 Zea mays

Organism

EC Number Organism UniProt Comment Textmining
1.8.7.1 Zea mays
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.7.1 sulfite + reduced ferredoxin
-
Zea mays hydrogen sulfide + oxidized ferredoxin + H2O
-
?

Subunits

EC Number Subunits Comment Organism
1.8.7.1 monomer 1 * 65000 Zea mays

Synonyms

EC Number Synonyms Comment Organism
1.8.7.1 ferredoxin and sulfite reductase
-
Zea mays
1.8.7.1 SIR
-
Zea mays

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.8.7.1 2.1
-
reduced ferredoxin using E92Q/E93Q/E29Q/D34N mutant ferredoxin, the reaction mixture contains regulator of fumarate and nitrate reduction (100 nM), sulfite reductase (ferredoxin) (800 nM), and sulfite (0.5 mM) in 30 mM potassium phosphate buffer, pH 7.0 and 25 mM NaCl Zea mays
1.8.7.1 2.66
-
reduced ferredoxin using E92Q/E30Q/D34N mutant ferredoxin, the reaction mixture contains regulator of fumarate and nitrate reduction (100 nM), sulfite reductase (ferredoxin) (800 nM), and sulfite (0.5 mM) in 30 mM potassium phosphate buffer, pH 7.0 and 25 mM NaCl Zea mays
1.8.7.1 2.7
-
reduced ferredoxin using E92Q/E93Q mutant ferredoxin, the reaction mixture contains regulator of fumarate and nitrate reduction (100 nM), sulfite reductase (ferredoxin) (800 nM), and sulfite (0.5 mM) in 30-mM potassium phosphate buffer, pH 7.0 and 25 mM NaCl Zea mays
1.8.7.1 3.65
-
reduced ferredoxin using wild type ferredoxin, the reaction mixture contains ferredoxin-NADP+ reductase (100 nM), sulfite reductase (ferredoxin) (800 nM), and sulfite (0.5 mM) in 30 mM potassium phosphate buffer, pH 7.0 and 25 mM NaCl Zea mays

Cofactor

EC Number Cofactor Comment Organism Structure
1.8.7.1 siroheme
-
Zea mays