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Literature summary extracted from

  • Waletko, A.; Zwicker, K.; Abdrakhmanova, A.; Zickermann, V.; Brandt, U.; Kerscher, S.
    Histidine 129 in the 75-kDa subunit of mitochondrial complex I from Yarrowia lipolytica is not a ligand for [Fe4S4] cluster N5 but is required for catalytic activity (2005), J. Biol. Chem., 280, 5622-5625.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
7.1.1.2 H129A fully assembled but destabilized enzyme, without deamino-NADH:ubiquinone oxidoreductase activity Yarrowia lipolytica

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
7.1.1.2 mitochondrion
-
 Yarrowia lipolytica 5739
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
7.1.1.2 4Fe-4S
-
 Yarrowia lipolytica

Organism

EC Number Organism UniProt Comment Textmining
7.1.1.2 Yarrowia lipolytica
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
7.1.1.2 Ni2+-affinity chromatography and gel filtration Yarrowia lipolytica

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
7.1.1.2 0.62
-
 mutant enzyme H129A, using hexamineruthenium(III)-chloride as substrate Yarrowia lipolytica
7.1.1.2 0.84
-
 wild type enzyme, using hexamineruthenium(III)-chloride as substrate Yarrowia lipolytica

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7.1.1.2 NADH + hexamineruthenium(III)-chloride
-
 Yarrowia lipolytica NAD+ + ?
-
 ?
7.1.1.2 NADH + ubiquinone
-
 Yarrowia lipolytica NAD+ + ubiquinol
-
 ?

Synonyms

EC Number Synonyms Comment Organism
7.1.1.2 complex I
-
 Yarrowia lipolytica
7.1.1.2 NADH:ubiquinone oxidoreductase
-
 Yarrowia lipolytica

Cofactor

EC Number Cofactor Comment Organism Structure
7.1.1.2 NADH
-
 Yarrowia lipolytica