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Literature summary extracted from

  • Oria-Hernandez, J.; Cabrera, N.; Perez-Montfort, R.; Ramirez-Silva, L.
    Pyruvate kinase revisited: the activating effect of K+ (2005), J. Biol. Chem., 280, 37924-37929.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.40 expressed in Escherichia coli Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.7.1.40 E117K decreased activity Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.40 ADP-Cr2+
-
 Homo sapiens
2.7.1.40 oxalate
-
 Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.40 0.13
-
 phosphoenolpyruvate wild type enzyme, in the presence of K+, in 50 mM Mes-Tris, pH 6.0, at 25°C Homo sapiens
2.7.1.40 0.24
-
 phosphoenolpyruvate mutant enzyme E117K, in the absence of K+, in 50 mM Mes-Tris, pH 6.0, at 25°C Homo sapiens
2.7.1.40 0.61
-
 phosphoenolpyruvate wild type enzyme, in the absence of K+, in 50 mM Mes-Tris, pH 6.0, at 25°C Homo sapiens
2.7.1.40 2
-
 ADP mutant enzyme E117K, in the absence of K+, in 50 mM Mes-Tris, pH 6.0, at 25°C Homo sapiens
2.7.1.40 2.3
-
 ADP wild type enzyme, in the presence of K+, in 50 mM Mes-Tris, pH 6.0, at 25°C Homo sapiens
2.7.1.40 14.1
-
 ADP wild type enzyme, in the absence of K+, in 50 mM Mes-Tris, pH 6.0, at 25°Cin 50 mM Mes-Tris, pH 6.0, at 25°C Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.40 K+ essential activator, in the presence of K+ the affinities for phosphoenolpyruvate, ADP, ADP-Cr2+, and oxalate are 2-6fold higher than in the absence of K+ when ADP cannot bind to the enzyme until phosphoenolpyruvate forms a competent active site Homo sapiens
2.7.1.40 Mg required for activity Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.40 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.40 DEAE column chromatography and carboxymethyl-Sepharose column chromatography Homo sapiens

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.40 0.02
-
 in the absence of K+ Homo sapiens
2.7.1.40 250
-
 in the presence of K+ Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.40 ADP + phosphoenolpyruvate
-
 Homo sapiens ATP + pyruvate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.40 tetramer x-ray crystallography Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
2.7.1.40 phosphoenolpyruvate kinase
-
 Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.1.40 3.2
-
 phosphoenolpyruvate wild type enzyme, in the absence of K+, in 50 mM Mes-Tris, pH 6.0, at 25°C Homo sapiens
2.7.1.40 161
-
 phosphoenolpyruvate mutant enzyme E117K, in the absence of K+, in 50 mM Mes-Tris, pH 6.0, at 25°C Homo sapiens
2.7.1.40 1182
-
 phosphoenolpyruvate wild type enzyme, in the presence of K+, in 50 mM Mes-Tris, pH 6.0, at 25°C Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.40 ADP
-
 Homo sapiens

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.1.40 0.0005
-
 ADP-Cr2+ mutant enzyme E117K, in the absence of K+, in 50 mM Mes-Tris, pH 6.0, at 25°C Homo sapiens
2.7.1.40 0.0013
-
 ADP-Cr2+ wild type enzyme, in the presence of K+, in 50 mM Mes-Tris, pH 6.0, at 25°C Homo sapiens
2.7.1.40 0.0028
-
 ADP-Cr2+ wild type enzyme, in the absence of K+, in 50 mM Mes-Tris, pH 6.0, at 25°C Homo sapiens
2.7.1.40 0.0048
-
 oxalate mutant enzyme E117K, in the absence of K+, in 50 mM Mes-Tris, pH 6.0, at 25°C Homo sapiens
2.7.1.40 0.029
-
 oxalate wild type enzyme, in the presence of K+, in 50 mM Mes-Tris, pH 6.0, at 25°C Homo sapiens
2.7.1.40 0.077
-
 oxalate wild type enzyme, in the absence of K+, in 50 mM Mes-Tris, pH 6.0, at 25°C Homo sapiens