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Literature summary extracted from

  • Christensen, K.E.; Mirza, I.A.; Berghuis, A.M.; Mackenzie, R.E.
    Magnesium and phosphate ions enable NAD binding to methylenetetrahydrofolate dehydrogenase-methenyltetrahydrofolate cyclohydrolase (2005), J. Biol. Chem., 280, 34316-34323.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.5.1.15 phosphate absolutely required, binds in close proximity to the 2’-hydroxyl of NAD through interactions with R166 and R198. Km-value of wild-type 0.412 mM Homo sapiens

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.5.1.15 construction of homology model Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
1.5.1.15 D133A cosubstrate NAD+, 1.7% of wild-type activity, cosubstrate NADP+, 5.5% of wild-type activity Homo sapiens
1.5.1.15 D133E no enzymic activity Homo sapiens
1.5.1.15 D133N cosubstrate NAD+, 20.4% of wild-type activity, cosubstrate NADP+, 82.2% of wild-type activity Homo sapiens
1.5.1.15 D133S cosubstrate NAD+, 17.8% of wild-type activity, cosubstrate NADP+, 34.5% of wild-type activity Homo sapiens
1.5.1.15 D190A no enzymic activity Homo sapiens
1.5.1.15 D190E cosubstrate NAD+, 1% of wild-type activity Homo sapiens
1.5.1.15 D190N cosubstrate NAD+, 16% of wild-type activity Homo sapiens
1.5.1.15 D190S no enzymic activity Homo sapiens
1.5.1.15 R166A no enzymic activity Homo sapiens
1.5.1.15 R166K no enzymic activity Homo sapiens
1.5.1.15 R166S no enzymic activity Homo sapiens
1.5.1.15 R198A cosubstrate NAD+, 0.7% of wild-type activity, cosubstrate NADP+, 5.3% of wild-type activity Homo sapiens
1.5.1.15 R198K cosubstrate NAD+, 56% of wild-type activity, cosubstrate NADP+, 42% of wild-type activity Homo sapiens
1.5.1.15 R198S cosubstrate NAD+, 0.9% of wild-type activity, cosubstrate NADP+, 53% of wild-type activity Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.1.15 0.202
-
 NAD+ wild-type, pH 7.3 Homo sapiens
1.5.1.15 0.255
-
 NADP+ mutant R198S, pH 7.3 Homo sapiens
1.5.1.15 0.352
-
 NADP+ wild-type, pH 7.3 Homo sapiens
1.5.1.15 0.503
-
 NADP+ mutant D133S, pH 7.3 Homo sapiens
1.5.1.15 0.58
-
 NADP+ mutant D133N, pH 7.3 Homo sapiens
1.5.1.15 0.689
-
 NADP+ mutant D190N, pH 7.3 Homo sapiens
1.5.1.15 0.918
-
 NAD+ mutant R198K, pH 7.3 Homo sapiens
1.5.1.15 1.99
-
 NAD+ mutant D133N, pH 7.3 Homo sapiens
1.5.1.15 2.12
-
 NADP+ mutant R198K, pH 7.3 Homo sapiens
1.5.1.15 2.17
-
 NAD+ mutant D133S, pH 7.3 Homo sapiens
1.5.1.15 3.88
-
 NAD+ mutant D190N, pH 7.3 Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.5.1.15 Mg2+ absolutely required Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.15 Homo sapiens P13995
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.5.1.15 0.024
-
 mutant D190E, cosubstrate NAD+, pH 7.3 Homo sapiens
1.5.1.15 0.156
-
 mutant R198A, cosubstrate NADP+, pH 7.3 Homo sapiens
1.5.1.15 0.16
-
 mutant D133A, cosubstrate NADP+, pH 7.3 Homo sapiens
1.5.1.15 0.17
-
 mutant R198A, cosubstrate NAD+, pH 7.3 Homo sapiens
1.5.1.15 0.207
-
 mutant R198S, cosubstrate NAD+, pH 7.3 Homo sapiens
1.5.1.15 0.392
-
 mutant D133A, cosubstrate NAD+, pH 7.3 Homo sapiens
1.5.1.15 1.01
-
 mutant D133S, cosubstrate NADP+, pH 7.3 Homo sapiens
1.5.1.15 1.24
-
 mutant R198K, cosubstrate NADP+, pH 7.3 Homo sapiens
1.5.1.15 1.54
-
 mutant R198S, cosubstrate NADP+, pH 7.3 Homo sapiens
1.5.1.15 2 2.5 wild-type, cosubstrate NAD+, pH 7.3 Homo sapiens
1.5.1.15 2.4
-
 mutant D133N, cosubstrate NADP+, pH 7.3 Homo sapiens
1.5.1.15 2.92
-
 wild-type, cosubstrate NADP+, pH 7.3 Homo sapiens
1.5.1.15 3.64
-
 mutant D190N, cosubstrate NAD+, pH 7.3 Homo sapiens
1.5.1.15 3.96
-
 mutant D190N, cosubstrate NADP+, pH 7.3 Homo sapiens
1.5.1.15 3.99
-
 mutant D133S, cosubstrate NAD+, pH 7.3 Homo sapiens
1.5.1.15 4.6
-
 mutant D133N, cosubstrate NAD+, pH 7.3 Homo sapiens
1.5.1.15 12.6
-
 mutant R198K, cosubstrate NAD+, pH 7.3 Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.15 5,10-methylenetetrahydrofolate + NAD+
-
 Homo sapiens 5,10-methenyltetrahydrofolate + NADH
-
 ?
1.5.1.15 5,10-methylenetetrahydrofolate + NADP+ about 15% of the rate with NAD+ Homo sapiens 5,10-methenyltetrahydrofolate + NADPH + H+
-
 ?