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Literature summary extracted from

  • Schnell, R.; Sandalova, T.; Hellman, U.; Lindqvist, Y.; Schneider, G.
    Siroheme- and [Fe4-S4]-dependent NirA from Mycobacterium tuberculosis is a sulfite reductase with a covalent Cys-Tyr bond in the active site (2005), J. Biol. Chem., 280, 27319-27328.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.8.7.1 medicine potential target for the development of antituberculosis agents Mycobacterium tuberculosis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.8.7.1 expressed in Escherichia coli Mycobacterium tuberculosis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.8.7.1 hanging drop vapour diffusion method using 0.1 M Tris-HCl, pH range 8.2-8.7, 0.2 M MgCl2, and 30% polyethylene glycol 4000 Mycobacterium tuberculosis

Protein Variants

EC Number Protein Variants Comment Organism
1.8.7.1 C161A catalytically impaired mutant, tested in an assay using the nonphysiological electron donor methyl viologen and sulfite as substrate Mycobacterium tuberculosis
1.8.7.1 C161S catalytically impaired mutant, tested in an assay using the nonphysiological electron donor methyl viologen and sulfite as substrate Mycobacterium tuberculosis
1.8.7.1 Y69A catalytically impaired mutant, tested in an assay using the nonphysiological electron donor methyl viologen and sulfite as substrate Mycobacterium tuberculosis
1.8.7.1 Y69F catalytically impaired mutant, tested in an assay using the nonphysiological electron donor methyl viologen and sulfite as substrate Mycobacterium tuberculosis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.8.7.1 [4Fe-4S] center
-
 Mycobacterium tuberculosis

Organism

EC Number Organism UniProt Comment Textmining
1.8.7.1 Mycobacterium tuberculosis P9WJ03
-
-
1.8.7.1 Mycobacterium tuberculosis H37Rv P9WJ03
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.8.7.1 Ni2+-nitrilotriacetic acid affinity matrix and Q-Sepharose column chromatography Mycobacterium tuberculosis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.8.7.1 additional information
-
 the enzyme has a specific activity of 2.0 units/mg protein Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.7.1 additional information NirA has a pronounced preference for sulfite over nitrite as substrate Mycobacterium tuberculosis ?
-
 ?
1.8.7.1 additional information NirA has a pronounced preference for sulfite over nitrite as substrate Mycobacterium tuberculosis H37Rv ?
-
 ?
1.8.7.1 sulfite + methyl viologen
-
 Mycobacterium tuberculosis sulfide + ?
-
 ?
1.8.7.1 sulfite + methyl viologen
-
 Mycobacterium tuberculosis H37Rv sulfide + ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.8.7.1 monomer x-ray crystallography Mycobacterium tuberculosis

Synonyms

EC Number Synonyms Comment Organism
1.8.7.1 ferredoxin-dependent sulfite reductase
-
 Mycobacterium tuberculosis
1.8.7.1 NirA
-
 Mycobacterium tuberculosis
1.8.7.1 siroheme- and [Fe4-S4]-dependent NirA
-
 Mycobacterium tuberculosis

Cofactor

EC Number Cofactor Comment Organism Structure
1.8.7.1 siroheme
-
 Mycobacterium tuberculosis