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Literature summary extracted from

  • Nogaj, L.A.; Beale, S.I.
    Physical and kinetic interactions between glutamyl-tRNA reductase and glutamate-1-semialdehyde aminotransferase of Chlamydomonas reinhardtii (2005), J. Biol. Chem., 280, 24301-24307.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.2.1.70 glutamate-1-semialdehyde aminotransferase 2.5fold activity in presence of glutamate-1-semialdehyde aminotransferase Chlamydomonas reinhardtii

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.2.1.70 expression in Escherichia coli Chlamydomonas reinhardtii

Protein Variants

EC Number Protein Variants Comment Organism
1.2.1.70 C48A no activity Chlamydomonas reinhardtii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.1.70 46000
-
 2 * 52500 + 2 * 46000, sucrose gradient sedimentation of glutamyl-tRNA reductase preincubated with glutamate-1-semialdehyde aminotransferase. Both enzyme also co-precipitate in immunoprecipitation experiments Chlamydomonas reinhardtii
1.2.1.70 52500
-
 2 * 52500 + 2 * 46000, sucrose gradient sedimentation of glutamyl-tRNA reductase preincubated with glutamate-1-semialdehyde aminotransferase. Both enzyme also co-precipitate in immunoprecipitation experiments Chlamydomonas reinhardtii
1.2.1.70 52500
-
 2 * 52500, sucrose gradient sedimentation compared to calculation from sequence Chlamydomonas reinhardtii
1.2.1.70 101000
-
 sucrose density gradient sedimentation, dimer Chlamydomonas reinhardtii

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.70 Chlamydomonas reinhardtii
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.70 Ni-affinity chromatography Chlamydomonas reinhardtii

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.2.1.70 cell culture
-
 Chlamydomonas reinhardtii
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.70 L-glutamyl-tRNAGlu + NADH + H+
-
 Chlamydomonas reinhardtii L-glutamate 1-semialdehyde + NAD+ + tRNAGlu
-
 ?

Subunits

EC Number Subunits Comment Organism
1.2.1.70 dimer 2 * 52500, sucrose gradient sedimentation compared to calculation from sequence Chlamydomonas reinhardtii
1.2.1.70 heterotetramer 2 * 52500 + 2 * 46000, sucrose gradient sedimentation of glutamyl-tRNA reductase preincubated with glutamate-1-semialdehyde aminotransferase. Both enzyme also co-precipitate in immunoprecipitation experiments Chlamydomonas reinhardtii

Synonyms

EC Number Synonyms Comment Organism
1.2.1.70 glutamyl-tRNA reductase
-
 Chlamydomonas reinhardtii
1.2.1.70 GTR
-
 Chlamydomonas reinhardtii