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Literature summary extracted from

  • Okada, K.; Hase, T.
    Cyanobacterial non-mevalonate pathway: (E)-4-hydroxy-3-methylbut-2-enyl diphosphate synthase interacts with ferredoxin in Thermosynechococcus elongatus BP-1 (2005), J. Biol. Chem., 280, 20672-20679.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.17.7.1 expression in Escherichia coli Thermosynechococcus vestitus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.17.7.1 [4Fe-4S]2+ cluster [4Fe-4S]2 metalloprotein Thermosynechococcus vestitus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.17.7.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate + 2 reduced ferredoxin Thermosynechococcus vestitus essential enzyme of the non-mevalonate (2-C-methyl-D-erythritol-4-phosphate (MEP)) pathway for isoprenoid biosynthesis (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + H2O + 2 oxidized ferredoxin
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 ?

Organism

EC Number Organism UniProt Comment Textmining
1.17.7.1 Thermosynechococcus vestitus Q8DK70
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Purification (Commentary)

EC Number Purification (Comment) Organism
1.17.7.1 recombinant GcpE protein is purified by an N-terminal His6 tag and reconstituted as a [4Fe-4S]2 metalloprotein Thermosynechococcus vestitus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.17.7.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate + 2 reduced ferredoxin
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 Thermosynechococcus vestitus (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + H2O + 2 oxidized ferredoxin
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 ?
1.17.7.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate + 2 reduced ferredoxin essential enzyme of the non-mevalonate (2-C-methyl-D-erythritol-4-phosphate (MEP)) pathway for isoprenoid biosynthesis Thermosynechococcus vestitus (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + H2O + 2 oxidized ferredoxin
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 ?

Synonyms

EC Number Synonyms Comment Organism
1.17.7.1 GcpE ambiguous Thermosynechococcus vestitus

General Information

EC Number General Information Comment Organism
1.17.7.1 metabolism essential enzyme of the non-mevalonate (2-C-methyl-D-erythritol-4-phosphate (MEP)) pathway for isoprenoid biosynthesis Thermosynechococcus vestitus