Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Jin, C.; Barrientos, A.; Tzagoloff, A.
    Yeast dihydroxybutanone phosphate synthase, an enzyme of the riboflavin biosynthetic pathway, has a second unrelated function in expression of mitochondrial respiration (2003), J. Biol. Chem., 278, 14698-14703.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.99.12 gene RIB3, expression of the wild-type gene restores the ability to respire in the aE280/U1 pet mutant A137T of Saccharomyces cerevisiae, which is partially deficient in cytochromes a, a3, and cytochrome b Saccharomyces cerevisiae

Protein Variants

EC Number Protein Variants Comment Organism
4.1.99.12 A137T naturally occuring Rib3 mutant which is partially deficient in cytochromes a, a3, and cytochrome b, the respiratory defect elicited by this mutation cannot be explained by a flavin insufficiency based on the following evidence: 1. growth of the aE280/U1 on respiratory substrates is not rescued by exogenous riboflavin, 2. the levels of flavin nucleotides are not significantly different in the mutant and wild type, phenotype, overview Saccharomyces cerevisiae
4.1.99.12 additional information construction of RIB3 disruption mutants, restoration by riboflavin of growth of a rib3 deletion mutant on glucose but not glycerol/ethanol, phenotype, overview Saccharomyces cerevisiae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.1.99.12 cytosol mainly Saccharomyces cerevisiae 5829
-
4.1.99.12 mitochondrion in the intermembrane space, possibly associated with the outer membrane, minor enzyme portion Saccharomyces cerevisiae 5739
-
4.1.99.12 additional information subcellular localization analysis, overview Saccharomyces cerevisiae
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.99.12 22500
-
x * 28000, SDS-PAGE, x * 22500, about, sequence calculation Saccharomyces cerevisiae
4.1.99.12 28000
-
x * 28000, SDS-PAGE, x * 22500, about, sequence calculation Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.99.12 D-ribulose 5-phosphate Saccharomyces cerevisiae
-
formate + L-3,4-dihydroxy-2-butanone-4-phosphate
-
?
4.1.99.12 additional information Saccharomyces cerevisiae the enzyme is involved in the riboflavin biosynthetic pathway, but has a second unrelated function in expression of mitochondrial respiration ?
-
?

Organism

EC Number Organism UniProt Comment Textmining
4.1.99.12 Saccharomyces cerevisiae
-
gene RIB3, wild-type strain W303-1A
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.99.12 D-ribulose 5-phosphate
-
Saccharomyces cerevisiae formate + L-3,4-dihydroxy-2-butanone-4-phosphate
-
?
4.1.99.12 additional information the enzyme is involved in the riboflavin biosynthetic pathway, but has a second unrelated function in expression of mitochondrial respiration Saccharomyces cerevisiae ?
-
?

Subunits

EC Number Subunits Comment Organism
4.1.99.12 ? x * 28000, SDS-PAGE, x * 22500, about, sequence calculation Saccharomyces cerevisiae

Synonyms

EC Number Synonyms Comment Organism
4.1.99.12 DHBP synthase
-
Saccharomyces cerevisiae
4.1.99.12 dihydroxybutanone phosphate synthase
-
Saccharomyces cerevisiae
4.1.99.12 Rib3
-
Saccharomyces cerevisiae