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Literature summary extracted from

  • Abe, K.; Kaya, S.; Taniguchi, K.; Hayashi, Y.; Imagawa, T.; Kikumoto, M.; Oiwa, K.; Sakaguchi, K.
    Evidence for a relationship between activity and the tetraprotomeric assembly of solubilized pig gastric H/K-ATPase (2005), J. Biochem., 138, 293-301.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
7.2.2.19 additional information no protective effect during C12E8-solubilization of gastric H/K-ATPase by choline Sus scrofa

Application

EC Number Application Comment Organism
7.2.2.19 additional information strong evidence that the catalytic unit of C12E8-solubilized gastric H/K-ATPase is a tetraprotomer Sus scrofa

Inhibitors

EC Number Inhibitors Comment Organism Structure
7.2.2.19 Fluorescein 5'-isothiocyanate fluorescein 5'-isothiocyanate modified enzyme has 0.5-1.5% residual ATPase activity compared to the unmodified enzyme Sus scrofa
7.2.2.19 SCH28080
-
 Sus scrofa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
7.2.2.19 membrane
-
 Sus scrofa 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
7.2.2.19 K+ stabilizes both oligomeric assembly and enzyme activity in C12E8-solubilized gastric H/K-ATPase, 20-40 mM protects Sus scrofa
7.2.2.19 additional information no protective effect during C12E8-solubilization of gastric H/K-ATPase by Na+ and Li+ Sus scrofa
7.2.2.19 Rb+ protects gastric H/K-ATPase during C12E8-solubilization Sus scrofa

Organism

EC Number Organism UniProt Comment Textmining
7.2.2.19 Sus scrofa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
7.2.2.19 by SDS-PAGE Sus scrofa

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
7.2.2.19 6.3
-
 purified enzyme, 40 mM HEPES/Tris, 10 mM KCl, 2 mM MgCl2, 25 mM sucrose, 0.1 mM EGTA/Tris, 2 mM ATP/Tris pH 7.0, at 37°C Sus scrofa
7.2.2.19 7.5
-
 purified enzyme, 40 mM HEPES/Tris, 10 mM KCl, 2 mM MgCl2, 25 mM sucrose, 0.1 mM EGTA/Tris, 2 mM ATP/Tris pH 7.0, at 37°C Sus scrofa

Storage Stability

EC Number Storage Stability Organism
7.2.2.19 -80°C, EGTA/Tris buffer, pH 7.4 Sus scrofa

Synonyms

EC Number Synonyms Comment Organism
7.2.2.19 gastric H/K-ATPase
-
 Sus scrofa