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Literature summary extracted from

  • Ohmae, E.; Fukumizu, Y.; Iwakura, M.; Gekko, K.
    Effects of mutation at methionine-42 of Escherichia coli dihydrofolate reductase on stability and function: implication of hydrophobic interactions (2005), J. Biochem., 137, 643-652.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
1.5.1.3 M42A 45% decrease in ratio kcat/Km, analysis of thermodynamic parameters for urea denaturation Escherichia coli
1.5.1.3 M42C slight decrease in ratio kcat/Km, analysis of thermodynamic parameters for urea denaturation Escherichia coli
1.5.1.3 M42E 6fold decrease in ratio kcat/Km, analysis of thermodynamic parameters for urea denaturation Escherichia coli
1.5.1.3 M42G 45% decrease in ratio kcat/Km, analysis of thermodynamic parameters for urea denaturation Escherichia coli
1.5.1.3 M42H 40% decrease in ratio kcat/Km, analysis of thermodynamic parameters for urea denaturation Escherichia coli
1.5.1.3 M42I slight increase in ratio kcat/Km, analysis of thermodynamic parameters for urea denaturation Escherichia coli
1.5.1.3 M42L slight decrease in ratio kcat/Km, analysis of thermodynamic parameters for urea denaturation Escherichia coli
1.5.1.3 M42P 50% increase in ratio kcat/Km, analysis of thermodynamic parameters for urea denaturation Escherichia coli
1.5.1.3 M42Q 45% decrease in ratio kcat/Km, analysis of thermodynamic parameters for urea denaturation Escherichia coli
1.5.1.3 M42S 45% decrease in ratio kcat/Km, analysis of thermodynamic parameters for urea denaturation Escherichia coli
1.5.1.3 M42T slight increase in ratio kcat/Km, analysis of thermodynamic parameters for urea denaturation Escherichia coli
1.5.1.3 M42V slight decrease in ratio kcat/Km, analysis of thermodynamic parameters for urea denaturation Escherichia coli
1.5.1.3 M42W 6fold decrease in ratio kcat/Km, analysis of thermodynamic parameters for urea denaturation Escherichia coli
1.5.1.3 M42Y 30% increase in ratio kcat/Km, analysis of thermodynamic parameters for urea denaturation Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.1.3 0.00061
-
 7,8-dihydrofolate mutant M42I, pH 7.0, 25°C Escherichia coli
1.5.1.3 0.00082
-
 7,8-dihydrofolate mutant M42T, pH 7.0, 25°C Escherichia coli
1.5.1.3 0.00083
-
 7,8-dihydrofolate mutant M42P, pH 7.0, 25°C Escherichia coli
1.5.1.3 0.00091
-
 7,8-dihydrofolate mutant M42V, pH 7.0, 25°C Escherichia coli
1.5.1.3 0.00112
-
 7,8-dihydrofolate mutant M42L, pH 7.0, 25°C Escherichia coli
1.5.1.3 0.00118
-
 7,8-dihydrofolate mutant M42G, pH 7.0, 25°C Escherichia coli
1.5.1.3 0.0013
-
 7,8-dihydrofolate wild-type, pH 7.0, 25°C Escherichia coli
1.5.1.3 0.0013
-
 7,8-dihydrofolate mutant M42A, pH 7.0, 25°C Escherichia coli
1.5.1.3 0.00178
-
 7,8-dihydrofolate mutant M42Y, pH 7.0, 25°C Escherichia coli
1.5.1.3 0.00193
-
 7,8-dihydrofolate mutant M42H, pH 7.0, 25°C Escherichia coli
1.5.1.3 0.00194
-
 7,8-dihydrofolate mutant M42C, pH 7.0, 25°C Escherichia coli
1.5.1.3 0.00227
-
 7,8-dihydrofolate mutant M42Q, pH 7.0, 25°C Escherichia coli
1.5.1.3 0.00227
-
 7,8-dihydrofolate mutant M42S, pH 7.0, 25°C Escherichia coli
1.5.1.3 0.00597
-
 7,8-dihydrofolate mutant M42E, pH 7.0, 25°C Escherichia coli
1.5.1.3 0.045
-
 7,8-dihydrofolate mutant M42W, pH 7.0, 25°C Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.3 Escherichia coli
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.3 7,8-dihydrofolate + NADPH
-
 Escherichia coli 5,6,7,8-tetrahydrofolate + NADP+
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.5.1.3 12.4
-
 7,8-dihydrofolate mutant M42I, pH 7.0, 25°C Escherichia coli
1.5.1.3 13
-
 7,8-dihydrofolate mutant M42G, pH 7.0, 25°C Escherichia coli
1.5.1.3 14
-
 7,8-dihydrofolate mutant M42A, pH 7.0, 25°C Escherichia coli
1.5.1.3 15.7
-
 7,8-dihydrofolate mutant M42V, pH 7.0, 25°C Escherichia coli
1.5.1.3 15.9
-
 7,8-dihydrofolate mutant M42E, pH 7.0, 25°C Escherichia coli
1.5.1.3 18.7
-
 7,8-dihydrofolate mutant M42T, pH 7.0, 25°C Escherichia coli
1.5.1.3 18.9
-
 7,8-dihydrofolate mutant M42L, pH 7.0, 25°C Escherichia coli
1.5.1.3 22.6
-
 7,8-dihydrofolate mutant M42S, pH 7.0, 25°C Escherichia coli
1.5.1.3 24
-
 7,8-dihydrofolate mutant M42P, pH 7.0, 25°C Escherichia coli
1.5.1.3 24.3
-
 7,8-dihydrofolate mutant M42Q, pH 7.0, 25°C Escherichia coli
1.5.1.3 24.6
-
 7,8-dihydrofolate wild-type, pH 7.0, 25°C Escherichia coli
1.5.1.3 26.6
-
 7,8-dihydrofolate mutant M42H, pH 7.0, 25°C Escherichia coli
1.5.1.3 30.4
-
 7,8-dihydrofolate mutant M42C, pH 7.0, 25°C Escherichia coli
1.5.1.3 45.5
-
 7,8-dihydrofolate mutant M42Y, pH 7.0, 25°C Escherichia coli
1.5.1.3 89
-
 7,8-dihydrofolate mutant M42W, pH 7.0, 25°C Escherichia coli
1.5.1.3 104.6
-
 7,8-dihydrofolate mutant M42W, pH 7.0, 25°C Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.1.3 NADPH
-
 Escherichia coli