EC Number | Cloned (Comment) | Organism |
---|---|---|
5.1.1.3 | cloned in Escherichia coli | Bacillus anthracis |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
5.1.1.3 | C185A | predicted active site is mutated by site-directed mutagenesis, detectable racemase activity is attenuated by at least 100fold | Bacillus anthracis |
5.1.1.3 | C188A | predicted active site is mutated by site-directed mutagenesis, detectable racemase activity is attenuated by at least 100fold | Bacillus anthracis |
5.1.1.3 | C74A | predicted active site is mutated by site-directed mutagenesis, detectable racemase activity is attenuated by at least 100fold | Bacillus anthracis |
5.1.1.3 | C77A | predicted active site is mutated by site-directed mutagenesis, detectable racemase activity is attenuated by at least 100fold | Bacillus anthracis |
5.1.1.3 | additional information | RacE1 and RacE2 share 51% sequence identity and 67% sequence similarity | Bacillus anthracis |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
5.1.1.3 | 0.77 | - |
D-glutamate | - |
Bacillus anthracis | |
5.1.1.3 | 1 | - |
D-glutamate | - |
Bacillus anthracis | |
5.1.1.3 | 14 | - |
L-glutamate | - |
Bacillus anthracis | |
5.1.1.3 | 38 | - |
L-glutamate | - |
Bacillus anthracis |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
5.1.1.3 | 32320 | - |
predicted cDNA | Bacillus anthracis |
5.1.1.3 | 32530 | - |
predicted from cDNA | Bacillus anthracis |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
5.1.1.3 | Bacillus anthracis | Q81LA8 | - |
- |
5.1.1.3 | Bacillus anthracis | Q81UL8 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
5.1.1.3 | RacE1 is expressed as in Escherichia coli with an amino-terminal hexahistidine fusion peptide to facilitate purification via nickel-chelate affinity chromatography | Bacillus anthracis |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
5.1.1.3 | additional information | - |
in Bacillus anthracis glutamate racemase is encoded by two genes racE1 and racE2, both enzymes are different in quaternary structure and catalyze the reversible stereoisomerization with similar but not identical kinetic properties | Bacillus anthracis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
5.1.1.3 | L-glutamate | - |
Bacillus anthracis | D-glutamate | - |
r |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
5.1.1.3 | monomer | gel filtration | Bacillus anthracis |
5.1.1.3 | More | although RacE1 and RacE2 share significant sequence similarity, these proteins have different quaternary structural properties | Bacillus anthracis |
5.1.1.3 | More | by gel filtration it is shown that in solution RacE2 may be polydisperse, existing as both monomers and higher-order complexes | Bacillus anthracis |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
5.1.1.3 | glutamate racemase | - |
Bacillus anthracis |
5.1.1.3 | RacE1 | - |
Bacillus anthracis |
5.1.1.3 | RacE2 | - |
Bacillus anthracis |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
5.1.1.3 | 25 | - |
assay at | Bacillus anthracis |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
5.1.1.3 | 1.8 | - |
D-glutamate | - |
Bacillus anthracis | |
5.1.1.3 | 2 | - |
D-glutamate | - |
Bacillus anthracis | |
5.1.1.3 | 12 | - |
L-glutamate | - |
Bacillus anthracis | |
5.1.1.3 | 18 | - |
L-glutamate | - |
Bacillus anthracis |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
5.1.1.3 | 8.1 | - |
maximum activity | Bacillus anthracis |
5.1.1.3 | 8.2 | - |
maximum activity | Bacillus anthracis |