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Literature summary extracted from

  • Ferraro, D.J.; Okerlund, A.L.; Mowers, J.C.; Ramaswamy, S.
    Structural basis for regioselectivity and stereoselectivity of product formation by naphthalene 1,2-dioxygenase (2006), J. Bacteriol., 188, 6986-6994.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.12.12 expressed in Escherichia coli DH5alpha cells Pseudomonas sp.

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.14.12.12 hanging drop vapour diffusion method Pseudomonas sp.

Protein Variants

EC Number Protein Variants Comment Organism
1.14.12.12 F352V mutation changes the product regioselectivities for naphthalene, phenanthrene, and biphenyl Pseudomonas sp.

Organism

EC Number Organism UniProt Comment Textmining
1.14.12.12 Pseudomonas sp.
-
-
-
1.14.12.12 Pseudomonas sp. NCIB 9816-4
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.12.12 Q-Sepharose FF column chromatography and 1 hydroxyapatite ion-exchange column chromatography Pseudomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.12.12 3-nitrotoluene + NADH + O2
-
 Pseudomonas sp. 4-nitrobenzyl alcohol + NAD+
-
 ?
1.14.12.12 3-nitrotoluene + NADH + O2
-
 Pseudomonas sp. NCIB 9816-4 4-nitrobenzyl alcohol + NAD+
-
 ?
1.14.12.12 anthracene + NADH + O2
-
 Pseudomonas sp. (1R,2S)-1,2-dihydroanthracene-1,2-diol + (1S,2R)-1,2-dihydroanthracene-1,2-diol + NAD+
-
 ?
1.14.12.12 anthracene + NADH + O2
-
 Pseudomonas sp. NCIB 9816-4 (1R,2S)-1,2-dihydroanthracene-1,2-diol + (1S,2R)-1,2-dihydroanthracene-1,2-diol + NAD+
-
 ?
1.14.12.12 biphenyl + NADH + O2
-
 Pseudomonas sp. ? + NAD+
-
 ?
1.14.12.12 biphenyl + NADH + O2
-
 Pseudomonas sp. NCIB 9816-4 ? + NAD+
-
 ?
1.14.12.12 naphthalene + NADH + O2
-
 Pseudomonas sp. (1R,2S)-1,2-dihydronaphthalene-1,2-diol + NAD+
-
 ?
1.14.12.12 naphthalene + NADH + O2
-
 Pseudomonas sp. NCIB 9816-4 (1R,2S)-1,2-dihydronaphthalene-1,2-diol + NAD+
-
 ?
1.14.12.12 phenanthrene + NADH + O2
-
 Pseudomonas sp. (1R,2S)-1,2-dihydrophenanthrene-1,2-diol + (1S,2R)-1,2-dihydrophenanthrene-1,2-diol + (3R,4S)-3,4-dihydrophenanthrene-3,4-diol + (3S,4R)-3,4-dihydrophenanthrene-3,4-diol + NAD+
-
 ?
1.14.12.12 phenanthrene + NADH + O2
-
 Pseudomonas sp. NCIB 9816-4 (1R,2S)-1,2-dihydrophenanthrene-1,2-diol + (1S,2R)-1,2-dihydrophenanthrene-1,2-diol + (3R,4S)-3,4-dihydrophenanthrene-3,4-diol + (3S,4R)-3,4-dihydrophenanthrene-3,4-diol + NAD+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.12.12 NDO
-
 Pseudomonas sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.12.12 NADH
-
 Pseudomonas sp.