Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Friedmann, S.; Alber, B.E.; Fuchs, G.
    Properties of succinyl-coenzyme A:D-citramalate coenzyme A transferase and its role in the autotrophic 3-hydroxypropionate cycle of Chloroflexus aurantiacus (2006), J. Bacteriol., 188, 6460-6468.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.8.3.20 heterologously expressed in Escherichia coli Chloroflexus aurantiacus
4.1.3.25 expressed in Escherichia coli BL21 cells Chloroflexus aurantiacus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.8.3.20 0.08
-
 succinyl-CoA 55°C, pH and temperature not specified in the publication Chloroflexus aurantiacus
2.8.3.20 2.2
-
 (R)-citramalate 55°C, pH and temperature not specified in the publication Chloroflexus aurantiacus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.8.3.20 additional information activity is not dependent on Mg2+ Chloroflexus aurantiacus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.8.3.20 44000
-
 2 * 44000, SDS-PAGE Chloroflexus aurantiacus
2.8.3.20 90000
-
 gel filtration Chloroflexus aurantiacus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.8.3.20 succinyl-CoA + (R)-citramalate Chloroflexus aurantiacus the enzyme catalyzes a steps in the glyoxylate assimilation route succinate + (R)-citramalyl-CoA
-
 ?
2.8.3.20 succinyl-CoA + (R)-citramalate Chloroflexus aurantiacus DSM 636 the enzyme catalyzes a steps in the glyoxylate assimilation route succinate + (R)-citramalyl-CoA
-
 ?
2.8.3.20 succinyl-CoA + (R)-citramalate Chloroflexus aurantiacus OK-70-fl / DSM 636 the enzyme catalyzes a steps in the glyoxylate assimilation route succinate + (R)-citramalyl-CoA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.8.3.20 Chloroflexus aurantiacus
-
-
-
2.8.3.20 Chloroflexus aurantiacus DSM 636
-
-
-
4.1.3.46 Chloroflexus aurantiacus
-
-
-
4.1.3.46 Chloroflexus aurantiacus OK-70-fl / DSM 636
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.8.3.20 partial Chloroflexus aurantiacus
4.1.3.46 ammonium sulfate precipitation, gel filtration, and Mono Q chromatography Chloroflexus aurantiacus

Reaction

EC Number Reaction Comment Organism Reaction ID
2.8.3.20 succinyl-CoA + (R)-citramalate = succinate + (R)-citramalyl-CoA (1) Chloroflexus aurantiacus
a
2.8.3.20 succinyl-CoA + (R)-malate = succinate + (R)-malyl-CoA (2) Chloroflexus aurantiacus
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.8.3.20 17.5
-
 55°C, pH and temperature not specified in the publication Chloroflexus aurantiacus
4.1.3.46 1.5
-
 recombinant enzyme Chloroflexus aurantiacus

Storage Stability

EC Number Storage Stability Organism
2.8.3.20 4°C, pH 7, 1 week, in the presence of 100 mM KCl, without significant loss of activity Chloroflexus aurantiacus
2.8.3.20 frozen for months in the presence of 10% glycerol (v/v), without significant loss of activity Chloroflexus aurantiacus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.8.3.20 succinyl-CoA + (R)-citramalate
-
 Chloroflexus aurantiacus succinate + (R)-citramalyl-CoA
-
 ?
2.8.3.20 succinyl-CoA + (R)-citramalate the enzyme catalyzes a steps in the glyoxylate assimilation route Chloroflexus aurantiacus succinate + (R)-citramalyl-CoA
-
 ?
2.8.3.20 succinyl-CoA + (R)-citramalate
-
 Chloroflexus aurantiacus DSM 636 succinate + (R)-citramalyl-CoA
-
 ?
2.8.3.20 succinyl-CoA + (R)-citramalate the enzyme catalyzes a steps in the glyoxylate assimilation route Chloroflexus aurantiacus DSM 636 succinate + (R)-citramalyl-CoA
-
 ?
2.8.3.20 succinyl-CoA + (R)-citramalate
-
 Chloroflexus aurantiacus OK-70-fl / DSM 636 succinate + (R)-citramalyl-CoA
-
 ?
2.8.3.20 succinyl-CoA + (R)-citramalate the enzyme catalyzes a steps in the glyoxylate assimilation route Chloroflexus aurantiacus OK-70-fl / DSM 636 succinate + (R)-citramalyl-CoA
-
 ?
2.8.3.20 succinyl-CoA + (R)-malate 88% compared to the activity with (R)-citramalate Chloroflexus aurantiacus succinate + (R)-malyl-CoA
-
 ?
2.8.3.20 succinyl-CoA + (R)-malate 88% compared to the activity with (R)-citramalate Chloroflexus aurantiacus DSM 636 succinate + (R)-malyl-CoA
-
 ?
2.8.3.20 succinyl-CoA + (R)-malate 88% compared to the activity with (R)-citramalate Chloroflexus aurantiacus OK-70-fl / DSM 636 succinate + (R)-malyl-CoA
-
 ?
2.8.3.20 succinyl-CoA + itaconate 80% compared to the activity with (R)-citramalate Chloroflexus aurantiacus succinate + ?
-
 ?
2.8.3.20 succinyl-CoA + itaconate 80% compared to the activity with (R)-citramalate Chloroflexus aurantiacus DSM 636 succinate + ?
-
 ?
2.8.3.20 succinyl-CoA + itaconate 80% compared to the activity with (R)-citramalate Chloroflexus aurantiacus OK-70-fl / DSM 636 succinate + ?
-
 ?
4.1.3.46 (3R)-citramalyl-CoA
-
 Chloroflexus aurantiacus acetyl-CoA + pyruvate
-
 ?
4.1.3.46 (3R)-citramalyl-CoA
-
 Chloroflexus aurantiacus DSM 636 acetyl-CoA + pyruvate
-
 ?
4.1.3.46 (3R)-citramalyl-CoA
-
 Chloroflexus aurantiacus OK-70-fl / DSM 636 acetyl-CoA + pyruvate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.8.3.20 homodimer 2 * 44000, SDS-PAGE Chloroflexus aurantiacus

Synonyms

EC Number Synonyms Comment Organism
2.8.3.20 SCT
-
 Chloroflexus aurantiacus
4.1.3.25 D-citramalyl-CoA lyase
-
 Chloroflexus aurantiacus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.8.3.20 55
-
-
 Chloroflexus aurantiacus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.8.3.20 55
-
 stable Chloroflexus aurantiacus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.8.3.20 13
-
 succinyl-CoA 55°C, pH and temperature not specified in the publication Chloroflexus aurantiacus
2.8.3.20 13
-
 (R)-citramalate 55°C, pH and temperature not specified in the publication Chloroflexus aurantiacus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.8.3.20 6.5
-
 at 55°C Chloroflexus aurantiacus

Expression

EC Number Organism Comment Expression
2.8.3.20 Chloroflexus aurantiacus upregulated under autotrophic conditions up

General Information

EC Number General Information Comment Organism
2.8.3.20 physiological function the enzyme catalyzes a steps in the glyoxylate assimilation route Chloroflexus aurantiacus