Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Gruendling, A.; Missiakas, D.M.; Schneewind, O.
    Staphylococcus aureus mutants with increased lysostaphin resistance (2006), J. Bacteriol., 188, 6286-6297.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.24.75 expression of His-tagged lysostaphin comprising residues 1 to 154 in Escherichia coli strain BL21(DE3) Staphylococcus simulans

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.24.75 extracellular the enzyme is secreted Staphylococcus simulans
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.24.75 additional information Staphylococcus simulans Staphylococcus simulans secretes lysostaphin, a bacteriolytic enzyme that specifically binds to the cell wall envelope of Staphylococcus aureus and cleaves the pentaglycine cross bridges of peptidoglycan, thereby killing staphylococci, screening of a collection of Staphylococcus aureus strain Newman transposon mutants for lysostaphin resistance, transposon insertion in SAV2335 is herein named lyrA, i.e. lysostaphin resistance A, activity against diverse Staphylococcus aureus strains with MIC-values of 0.0005-0.002 mg/ml, overview ?
-
 ?
3.4.24.75 additional information Staphylococcus simulans TNK1 Staphylococcus simulans secretes lysostaphin, a bacteriolytic enzyme that specifically binds to the cell wall envelope of Staphylococcus aureus and cleaves the pentaglycine cross bridges of peptidoglycan, thereby killing staphylococci, screening of a collection of Staphylococcus aureus strain Newman transposon mutants for lysostaphin resistance, transposon insertion in SAV2335 is herein named lyrA, i.e. lysostaphin resistance A, activity against diverse Staphylococcus aureus strains with MIC-values of 0.0005-0.002 mg/ml, overview ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.6.5 Staphylococcus aureus
-
-
-
3.4.24.75 Staphylococcus simulans
-
-
-
3.4.24.75 Staphylococcus simulans TNK1
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.24.75 recombinant His-tagged lysostaphin comprising residues 1 to 154 from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Staphylococcus simulans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.75 additional information Staphylococcus simulans secretes lysostaphin, a bacteriolytic enzyme that specifically binds to the cell wall envelope of Staphylococcus aureus and cleaves the pentaglycine cross bridges of peptidoglycan, thereby killing staphylococci, screening of a collection of Staphylococcus aureus strain Newman transposon mutants for lysostaphin resistance, transposon insertion in SAV2335 is herein named lyrA, i.e. lysostaphin resistance A, activity against diverse Staphylococcus aureus strains with MIC-values of 0.0005-0.002 mg/ml, overview Staphylococcus simulans ?
-
 ?
3.4.24.75 additional information Staphylococcus simulans secretes lysostaphin, a bacteriolytic enzyme that specifically binds to the cell wall envelope of Staphylococcus aureus and cleaves the pentaglycine cross bridges of peptidoglycan, thereby killing staphylococci, screening of a collection of Staphylococcus aureus strain Newman transposon mutants for lysostaphin resistance, transposon insertion in SAV2335 is herein named lyrA, i.e. lysostaphin resistance A, activity against diverse Staphylococcus aureus strains with MIC-values of 0.0005-0.002 mg/ml, overview Staphylococcus simulans TNK1 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.6.5 GTP pyrophosphokinase
-
 Staphylococcus aureus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.24.75 37
-
 in vivo assay at Staphylococcus simulans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.24.75 7.5
-
 in vivo assay at Staphylococcus simulans