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Literature summary extracted from

  • Fortin, P.D.; Horsman, G.P.; Yang, H.M.; Eltis, L.D.
    A glutathione S-transferase catalyzes the dehalogenation of inhibitory metabolites of polychlorinated biphenyls (2006), J. Bacteriol., 188, 4424-4430.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.18 gene bphK, expression in Escherichia coli strain GJ1158 Paraburkholderia xenovorans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.18 additional information
-
 additional information steady-state kinetics using 1-chloro-2,4-dinitrobenzene as substrate Paraburkholderia xenovorans
2.5.1.18 0.11
-
 glutathione pH 6.5, 25°C, recombinant enzyme, with (2Z,4E)-3-chloro-2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid Paraburkholderia xenovorans
2.5.1.18 0.37
-
 glutathione pH 6.5, 25°C, recombinant enzyme, with (2E,4Z)-5-chloro-2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid Paraburkholderia xenovorans
2.5.1.18 0.39
-
 glutathione pH 6.5, 25°C, recombinant enzyme, with (2Z,4E)-3-chloro-6-(3,5-dichlorophenyl)-2-hydroxy-6-oxohexa-2,4-dienoic acid Paraburkholderia xenovorans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.18 glutathione + (2E,4Z)-5-chloro-2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid Paraburkholderia xenovorans
-
 chloride + 5-glutathionyl-2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid
-
 ?
2.5.1.18 glutathione + (2Z,4E)-3-chloro-2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid Paraburkholderia xenovorans
-
 chloride + 3-glutathionyl-2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid
-
 ?
2.5.1.18 glutathione + (2Z,4E)-3-chloro-6-(3,5-dichlorophenyl)-2-hydroxy-6-oxohexa-2,4-dienoic acid Paraburkholderia xenovorans
-
 ?
-
 ?
2.5.1.18 glutathione + 1-chloro-2,4-dinitrobenzene Paraburkholderia xenovorans
-
 chloride + 2,4-dinitrophenyl-glutathione
-
 ?
2.5.1.18 additional information Paraburkholderia xenovorans the enzyme catalyzes the dehalogenation of inhibitory metabolites of polychlorinated biphenyls and may be responsible for facilitation of PCB degradation by the bph pathway, overview ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.18 Paraburkholderia xenovorans
-
 gene bphK
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.18 recombinant BphK from Escherichia coli strain GJ1158 by glutathione affinity chromatography Paraburkholderia xenovorans

Reaction

EC Number Reaction Comment Organism Reaction ID
2.5.1.18 RX + glutathione = HX + R-S-glutathione the enzyme uses a rapid-equilibrium random-order ternary-complex mechanism or a compulsory order ternary-complex mechanism Paraburkholderia xenovorans
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.18 glutathione + (2E,4Z)-5-chloro-2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid
-
 Paraburkholderia xenovorans chloride + 5-glutathionyl-2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid
-
 ?
2.5.1.18 glutathione + (2Z,4E)-3-chloro-2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid
-
 Paraburkholderia xenovorans chloride + 3-glutathionyl-2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid
-
 ?
2.5.1.18 glutathione + (2Z,4E)-3-chloro-2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid preferred polychlorinated biphenyl metabolite substrate Paraburkholderia xenovorans chloride + 3-glutathionyl-2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid
-
 ?
2.5.1.18 glutathione + (2Z,4E)-3-chloro-6-(3,5-dichlorophenyl)-2-hydroxy-6-oxohexa-2,4-dienoic acid
-
 Paraburkholderia xenovorans ?
-
 ?
2.5.1.18 glutathione + 1-chloro-2,4-dinitrobenzene
-
 Paraburkholderia xenovorans chloride + 2,4-dinitrophenyl-glutathione
-
 ?
2.5.1.18 additional information the enzyme catalyzes the dehalogenation of inhibitory metabolites of polychlorinated biphenyls and may be responsible for facilitation of PCB degradation by the bph pathway, overview Paraburkholderia xenovorans ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.5.1.18 BphK
-
 Paraburkholderia xenovorans
2.5.1.18 glutathione S-transferase
-
 Paraburkholderia xenovorans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.18 25
-
 assay at Paraburkholderia xenovorans

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.5.1.18 0.01
-
 glutathione pH 6.5, 25°C, recombinant enzyme, with (2Z,4E)-3-chloro-6-(3,5-dichlorophenyl)-2-hydroxy-6-oxohexa-2,4-dienoic acid Paraburkholderia xenovorans
2.5.1.18 0.16
-
 glutathione pH 6.5, 25°C, recombinant enzyme, with (2Z,4E)-3-chloro-2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid Paraburkholderia xenovorans
2.5.1.18 0.24
-
 glutathione pH 6.5, 25°C, recombinant enzyme, with (2E,4Z)-5-chloro-2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid Paraburkholderia xenovorans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.18 6.5
-
 assay at Paraburkholderia xenovorans