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Literature summary extracted from

  • Terrak, M.; Nguyen-Disteche, M.
    Kinetic characterization of the monofunctional glycosyltransferase from Staphylococcus aureus (2006), J. Bacteriol., 188, 2528-2532.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.129 a soluble form of Staphylococcus aureus MGT devoid of its membrane anchor, called SauH6-MGT (D68-R268), is expressed in the cytoplasm of Escherichia coli C41 (DE3) strain Staphylococcus aureus

Protein Variants

EC Number Protein Variants Comment Organism
2.4.1.129 E100Q mutant of the soluble form of Staphylococcus aureus MGT devoid of its membrane anchor, called SauH6-MGT. glycosyltransferase activity of the mutant is reduced 500fold compared to the wild type Staphylococcus aureus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.129 EDTA the enzyme is almost inactive in presence of EDTA Staphylococcus aureus
2.4.1.129 Moenomycin 75 nM, 50% inhibition Staphylococcus aureus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.129 0.002
-
 undecaprenyl-diphosphoryl-MurNAc-(L-Ala-gamma-D-Glu-meso-(diaminopimelic acid)-D-Ala-D-Ala)-GlcNAc pH 7.5, 10 mM Mn2+ Staphylococcus aureus
2.4.1.129 0.0028
-
 undecaprenyl-diphosphoryl-MurNAc-(L-Ala-gamma-D-Glu-meso-(diaminopimelic acid)-D-Ala-D-Ala)-GlcNAc pH 6.0, 10 mM Mn2+ Staphylococcus aureus
2.4.1.129 0.0076
-
 undecaprenyl-diphosphoryl-MurNAc-(L-Ala-gamma-D-Glu-meso-(diaminopimelic acid)-D-Ala-D-Ala)-GlcNAc pH 7.5, 50 mM Mg2+ Staphylococcus aureus
2.4.1.129 0.008
-
 undecaprenyl-diphosphoryl-MurNAc-(L-Ala-gamma-D-Glu-meso-(diaminopimelic acid)-D-Ala-D-Ala)-GlcNAc pH 7.5, 10 mM Mg2+ Staphylococcus aureus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.129 Ca2+ 10 mM, stimulation Staphylococcus aureus
2.4.1.129 Mg2+ 50 mM, required for optimal activity Staphylococcus aureus
2.4.1.129 Mn2+ 10 mM required for optimal activity Staphylococcus aureus

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.129 Staphylococcus aureus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.129 soluble form of Staphylococcus aureus MGT devoid of its membrane anchor, called SauH6-MGT (D68-R268), is expressed in the cytoplasm of Escherichia coli C41 (DE3) strain Staphylococcus aureus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.129 undecaprenyl-diphosphoryl-MurNAc-(L-Ala-gamma-D-Glu-meso-(diaminopimelic acid)-D-Ala-D-Ala)-GlcNAc + undecaprenyl-diphosphoryl-MurNAc-(L-Ala-gamma-D-Glu-meso-(diaminopimelic acid)-D-Ala-D-Ala)-GlcNAc
-
 Staphylococcus aureus ?
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.1.129 0.0063
-
 undecaprenyl-diphosphoryl-MurNAc-(L-Ala-gamma-D-Glu-meso-(diaminopimelic acid)-D-Ala-D-Ala)-GlcNAc pH 6.0, 10 mM Mn2+ Staphylococcus aureus
2.4.1.129 0.0076
-
 undecaprenyl-diphosphoryl-MurNAc-(L-Ala-gamma-D-Glu-meso-(diaminopimelic acid)-D-Ala-D-Ala)-GlcNAc pH 7.5, 50 mM Mg2+ Staphylococcus aureus
2.4.1.129 0.008
-
 undecaprenyl-diphosphoryl-MurNAc-(L-Ala-gamma-D-Glu-meso-(diaminopimelic acid)-D-Ala-D-Ala)-GlcNAc pH 7.5, 10 mM Mg2+ Staphylococcus aureus
2.4.1.129 0.013
-
 undecaprenyl-diphosphoryl-MurNAc-(L-Ala-gamma-D-Glu-meso-(diaminopimelic acid)-D-Ala-D-Ala)-GlcNAc pH 7.5, 10 mM Mn2+ Staphylococcus aureus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.1.129 7.5
-
-
 Staphylococcus aureus