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Literature summary extracted from

  • Howell, D.M.; Xu, H.; White, R.H.
    (R)-citramalate synthase in methanogenic archaea (1999), J. Bacteriol., 181, 331-333.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.3.21 gene MJ1392 or cimA, DNA and amino acid sequence determination and analysis, functional overexpression in Escherichia coli strain BL21(DE3) Methanocaldococcus jannaschii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.3.21 acetyl-CoA + pyruvate + H2O Methanocaldococcus jannaschii the enzyme is involved in the biosynthesis of isoleucine CoA + (2R)-2-hydroxy-2-methylbutanedioate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.3.21 Methanocaldococcus jannaschii
-
 gene MJ1392 or cimA
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.3.21 recombinant CimA 26.5fold from Escherichia coli strain BL21(DE3) by ammonium sulfate fractionation and heat treatment at 60°C for 10 min, followed by gel filtration Methanocaldococcus jannaschii

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.3.21 2.87
-
 purified recombinant enzyme Methanocaldococcus jannaschii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.3.21 acetyl-CoA + pyruvate + H2O
-
 Methanocaldococcus jannaschii CoA + (2R)-2-hydroxy-2-methylbutanedioate (R)-citramalate is 2-methylmalate ?
2.3.3.21 acetyl-CoA + pyruvate + H2O the enzyme is involved in the biosynthesis of isoleucine Methanocaldococcus jannaschii CoA + (2R)-2-hydroxy-2-methylbutanedioate
-
 ?
2.3.3.21 additional information no activity with alpha-ketoglutarate, alpha-ketoadipate, alpha-ketopimelate, alpha-ketoisovalerate, and acetyl-CoA Methanocaldococcus jannaschii ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.3.21 CimA
-
 Methanocaldococcus jannaschii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.3.21 50
-
 assay at Methanocaldococcus jannaschii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.3.21 7.5
-
 assay at Methanocaldococcus jannaschii

Cofactor

EC Number Cofactor Comment Organism Structure
2.3.3.21 acetyl-CoA
-
 Methanocaldococcus jannaschii