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Literature summary extracted from

  • Turroni, S.; Vitali, B.; Bendazzoli, C.; Candela, M.; Gotti, R.; Federici, F.; Pirovano, F.; Brigidi, P.
    Oxalate consumption by lactobacilli: evaluation of oxalyl-CoA decarboxylase and formyl-CoA transferase activity in Lactobacillus acidophilus (2007), J. Appl. Microbiol., 103, 1600-1609.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.8.3.16 gene frc, from strain LA14, DNA and amino acid sequence determination and analysis, overexpression of N-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3) Lactobacillus acidophilus
4.1.1.8 expressed in Escherichia coli BL21(DE3) cells Lactobacillus acidophilus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.1.1.8 cytoplasm
-
 Lactobacillus acidophilus 5737
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.8.3.16 50000
-
 x * 50000, recombinant enzyme, SDS-PAGE Lactobacillus acidophilus
4.1.1.8 65000
-
 SDS-PAGE Lactobacillus acidophilus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.8.3.16 formyl-CoA + oxalate Lactobacillus acidophilus the enzyme is required for oxalate degradation together with oxalyl-CoA decarboxylase, EC 4.1.1.8, overview, contribution of Lactobacillus in regulating the intestinal oxalate homeostasis in the human host formate + oxalyl-CoA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.8.3.16 Lactobacillus acidophilus
-
 diverse strains, overview
-
4.1.1.8 Lactobacillus acidophilus A4PE17
-
-
4.1.1.8 Lactobacillus acidophilus LA14 A4PE17
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.8.3.16 formyl-CoA + oxalate
-
 Lactobacillus acidophilus formate + oxalyl-CoA
-
 ?
2.8.3.16 formyl-CoA + oxalate the enzyme is required for oxalate degradation together with oxalyl-CoA decarboxylase, EC 4.1.1.8, overview, contribution of Lactobacillus in regulating the intestinal oxalate homeostasis in the human host Lactobacillus acidophilus formate + oxalyl-CoA
-
 ?
4.1.1.8 Oxalyl-CoA
-
 Lactobacillus acidophilus Formyl-CoA + CO2
-
 ?
4.1.1.8 Oxalyl-CoA
-
 Lactobacillus acidophilus LA14 Formyl-CoA + CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
2.8.3.16 ? x * 50000, recombinant enzyme, SDS-PAGE Lactobacillus acidophilus

Synonyms

EC Number Synonyms Comment Organism
4.1.1.8 OXC
-
 Lactobacillus acidophilus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.8.3.16 37
-
 assay at Lactobacillus acidophilus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.8.3.16 6.8
-
 assay at Lactobacillus acidophilus

Cofactor

EC Number Cofactor Comment Organism Structure
4.1.1.8 thiamine diphosphate required Lactobacillus acidophilus