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Literature summary extracted from

  • Beauvais, L.G.; Lippard, S.J.
    Reactions of the peroxo intermediate of soluble methane monooxygenase hydroxylase with ethers (2005), J. Am. Chem. Soc., 127, 7370-7378.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.13.25 additional information
-
additional information analysis of kinetics of single turnover reactions of the two intermediates with ethyl vinyl ether and diethyl ether, single- and double-mixing stopped-flow optical spectroscopy, overview, stopped-flow kinetics, analytical model of transient kinetics Methylococcus capsulatus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.13.25 soluble
-
Methylococcus capsulatus
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.13.25 methane + NADH + O2 Methylococcus capsulatus
-
methanol + NAD+ + H2O
-
?
1.14.13.25 methane + NADH + O2 Methylococcus capsulatus Bath
-
methanol + NAD+ + H2O
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.25 Methylococcus capsulatus
-
-
-
1.14.13.25 Methylococcus capsulatus Bath
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.13.25 native MMOH, in the purification procedure includes anion exchange chromatography and gel filtration Methylococcus capsulatus

Reaction

EC Number Reaction Comment Organism Reaction ID
1.14.13.25 methane + NAD(P)H + H+ + O2 = methanol + NAD(P)+ + H2O the soluble enzyme utilizes a carboxylate-bridged diiron center and dioxygen, a di(my-oxo)diiron(IV) intermediate termed Q is responsible for the catalytic activity with hydrocarbons, the peroxodiiron(III) intermediate precedes Q formation in the catalytic cycle Methylococcus capsulatus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.13.25 0.02 0.04 purified MMOH, substrate propylene Methylococcus capsulatus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.25 methane + NADH + O2
-
Methylococcus capsulatus methanol + NAD+ + H2O
-
?
1.14.13.25 methane + NADH + O2
-
Methylococcus capsulatus Bath methanol + NAD+ + H2O
-
?
1.14.13.25 propylene + NADH + O2 the peroxodiiron(III) intermediate that precedes Q formation in the catalytic cycle has been demonstrated to react with propylene Methylococcus capsulatus propylene oxide + NAD+ + H2O
-
?
1.14.13.25 propylene + NADH + O2 the peroxodiiron(III) intermediate that precedes Q formation in the catalytic cycle has been demonstrated to react with propylene Methylococcus capsulatus Bath propylene oxide + NAD+ + H2O
-
?

Synonyms

EC Number Synonyms Comment Organism
1.14.13.25 MMOH
-
Methylococcus capsulatus
1.14.13.25 soluble methane monooxygenase hydroxylase
-
Methylococcus capsulatus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.13.25 45
-
assay at Methylococcus capsulatus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.13.25 7
-
assay at Methylococcus capsulatus

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.25 NADH
-
Methylococcus capsulatus