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Literature summary extracted from

  • Fan, F.; Gadda, G.
    Oxygen- and temperature-dependent kinetic isotope effects in choline oxidase: correlating reversible hydride transfer with environmentally enhanced tunneling (2005), J. Am. Chem. Soc., 127, 17954-17961.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.3.17 additional information
-
 additional information kinetics, recombinant enzyme, overview Arthrobacter globiformis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.3.17 choline + O2 Arthrobacter globiformis FAD-linked reaction betaine aldehyde + H2O2
-
 ?
1.1.3.17 FADH2 + O2 Arthrobacter globiformis
-
 FAD + H2O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.3.17 Arthrobacter globiformis
-
 strain ATCC 8010
-

Reaction

EC Number Reaction Comment Organism Reaction ID
1.1.3.17 choline + O2 = betaine aldehyde + H2O2 reaction mechanism via FAD, the enzyme catalyzes the four-electron-oxidation of choline to glycine betaine via the intermediate betaine aldehyde in two sequential FAD-dependent reaction steps, the enzyme correlates reversible hydride transfer with environmentally enhanced tunneling, oxygen- and temperature-dependent kinetic isotope effects, overview Arthrobacter globiformis
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.3.17 choline + O2 FAD-linked reaction Arthrobacter globiformis betaine aldehyde + H2O2
-
 ?
1.1.3.17 FADH2 + O2
-
 Arthrobacter globiformis FAD + H2O2
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.3.17 25
-
 assay at Arthrobacter globiformis

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.1.3.17 additional information
-
 temperature profile of the reaction catalyzed at different conditions, recombinant enzyme, overview Arthrobacter globiformis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.3.17 8
-
 assay at Arthrobacter globiformis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.3.17 additional information
-
 pH profile of the reaction catalyzed at different conditions, recombinant enzyme, overview Arthrobacter globiformis

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.3.17 FAD dependent on Arthrobacter globiformis