EC Number | Cloned (Comment) | Organism |
---|---|---|
1.14.15.1 | expression of wild-type and mutant enzymes in Escherichia coli | Pseudomonas putida |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.14.15.1 | T252A | site-sirected mutagenesis, comparison of substrate binding properties to the wild-type enzyme, overview | Pseudomonas putida |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.14.15.1 | additional information | - |
additional information | EPR/ENDOR studies of wild-type and mutant T252A enzymes | Pseudomonas putida |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.14.15.1 | Fe2+ | heme iron, formation of an oxyferrous cytochrome P450cam during reaction, structure of the active site of oxyferrous-P450cam | Pseudomonas putida |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.15.1 | (+)-camphor + O2 + reduced putidaredoxin | Pseudomonas putida | - |
(+)-exo-5-hydroxycamphor + oxidized putidaredoxin + H2O | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.15.1 | Pseudomonas putida | - |
- |
- |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
1.14.15.1 | (+)-camphor + reduced putidaredoxin + O2 = (+)-exo-5-hydroxycamphor + oxidized putidaredoxin + H2O | via H2O2 and a nucleophilic intermediate, the substrate can modulate the properties of both the monoxygenase active-oxygen intermediates and the proton-delivery network that encompasses them, the catalytic cycle, overview | Pseudomonas putida |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.15.1 | (+)-camphor + O2 + reduced putidaredoxin | - |
Pseudomonas putida | (+)-exo-5-hydroxycamphor + oxidized putidaredoxin + H2O | - |
? | |
1.14.15.1 | 5,5-difluorocamphor + O2 + reduced putidaredoxin | - |
Pseudomonas putida | ? | - |
? | |
1.14.15.1 | 5-methylenyl-camphor + O2 + reduced putidaredoxin | - |
Pseudomonas putida | ? | - |
? | |
1.14.15.1 | adamantanone + O2 + reduced putidaredoxin | - |
Pseudomonas putida | ? | - |
? | |
1.14.15.1 | additional information | the properties and reactivity of the oxyheme and of both the primary and the annealed intermediates are modulated by a bound substrate, including alterations in the properties of the heme center, the presence of any alternative substrate increases the lifetime of hydroperoxoferri-P450cam no less than about 20fold, especially 5-methylenyl-camphor | Pseudomonas putida | ? | - |
? | |
1.14.15.1 | norcamphor + O2 + reduced putidaredoxin | - |
Pseudomonas putida | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.14.15.1 | cytochrome p450cam | - |
Pseudomonas putida |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.14.15.1 | 8 | - |
assay at | Pseudomonas putida |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.14.15.1 | putidaredoxin | - |
Pseudomonas putida |