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Literature summary extracted from

  • Gherman, B.F.; Lippard, S.J.; Friesner, R.A.
    Substrate hydroxylation in methane monooxygenase: quantitative modeling via mixed quantum mechanics/molecular mechanics techniques (2005), J. Am. Chem. Soc., 127, 1025-1037.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.13.25 additional information
-
 additional information kinetics, deuterium kinetic isotope effects, overview Methylococcus capsulatus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.13.25 methane + NADH + O2 Methylococcus capsulatus
-
 methanol + NAD+ + H2O
-
 ?
1.14.13.25 methane + NADH + O2 Methylococcus capsulatus Bath
-
 methanol + NAD+ + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.25 Methylococcus capsulatus
-
-
-
1.14.13.25 Methylococcus capsulatus Bath
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
1.14.13.25 methane + NAD(P)H + H+ + O2 = methanol + NAD(P)+ + H2O reaction mechanism via carboxylate-bridged diiron center and dioxygen, a di(my-oxo)diiron(IV) intermediate termed Q is responsible for the catalytic activity with hydrocarbons, the peroxodiiron(III) intermediate precedes Q formation in the catalytic cycle, which consists of four principal steps, overview Methylococcus capsulatus
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.13.25 additional information
-
 quantitative modeling of substrate hydroxylation via mixed quantum mechanics/molecular mechanics techniques, overview Methylococcus capsulatus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.25 methane + NADH + O2
-
 Methylococcus capsulatus methanol + NAD+ + H2O
-
 ?
1.14.13.25 methane + NADH + O2
-
 Methylococcus capsulatus Bath methanol + NAD+ + H2O
-
 ?
1.14.13.25 additional information a number of substituted methanes, e.g. CH3X (X) H, CH3, OH, CN, NO2, or F, react with MMOH, quantitative modeling of substrate hydroxylation via mixed quantum mechanics/molecular mechanics techniques, overview Methylococcus capsulatus ?
-
 ?
1.14.13.25 additional information a number of substituted methanes, e.g. CH3X (X) H, CH3, OH, CN, NO2, or F, react with MMOH, quantitative modeling of substrate hydroxylation via mixed quantum mechanics/molecular mechanics techniques, overview Methylococcus capsulatus Bath ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.13.25 MMO Bath
-
 Methylococcus capsulatus

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.25 NADH
-
 Methylococcus capsulatus