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Literature summary extracted from

  • Takeda, H.; Toyooka, T.; Ikeuchi, Y.; Yokobori, S.; Okadome, K.; Takano, F.; Oshima, T.; Suzuki, T.; Endo, Y.; Hori, H.
    The substrate specificity of tRNA (m1G37) methyltransferase (TrmD) from Aquifex aeolicus (2006), Genes Cells, 11, 1353-1365.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.228 expression in Escherichia coli Aquifex aeolicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.228 additional information
-
 additional information KM-values for truncated tRNAPhe variants Aquifex aeolicus
2.1.1.228 0.0004
-
 guanine37 in Aquifex aeolicus tRNAGln(UUG) pH 7.6, 60°C Aquifex aeolicus
2.1.1.228 0.0005
-
 guanine37 in Haloferax volcanii tRNALeu(CAA) pH 7.6, 60°C Aquifex aeolicus
2.1.1.228 0.0006
-
 guanine37 in Aquifex aeolicus tRNAHis(GUG) pH 7.6, 60°C Aquifex aeolicus
2.1.1.228 0.0006
-
 guanine37 in Aquifex aeolicus tRNAArg(ACG) pH 7.6, 60°C Aquifex aeolicus
2.1.1.228 0.0007
-
 guanine37 in Aquifex aeolicus tRNAPro(GGG) pH 7.6, 60°C Aquifex aeolicus
2.1.1.228 0.0008
-
 guanine37 in Aquifex aeolicus tRNAArg(CCG) pH 7.6, 60°C Aquifex aeolicus
2.1.1.228 0.0008
-
 guanine37 in yeast tRNAPhe(GAA) pH 7.6, 60°C Aquifex aeolicus
2.1.1.228 0.0009
-
 guanine37 in Aquifex aeolicus tRNALeu(CAG) pH 7.6, 60°C Aquifex aeolicus
2.1.1.228 0.0019
-
 guanine37 in Haloferax volcanii tRNATyr(GUA) pH 7.6, 60°C Aquifex aeolicus
2.1.1.228 0.002
-
 guanine37 in Haloferax volcanii tRNACys(GCA) pH 7.6, 60°C Aquifex aeolicus
2.1.1.228 0.0023
-
 guanine37 in Haloferax volcanii tRNATrp(CCA) pH 7.6, 60°C Aquifex aeolicus
2.1.1.228 0.0033
-
 guanine37 in in Escherichia coli tRNALeu(CAG) pH 7.6, 60°C Aquifex aeolicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.228 S-adenosyl-L-methionine + guanine37 in tRNA Aquifex aeolicus
-
 S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.228 Aquifex aeolicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.228
-
 Aquifex aeolicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.228 additional information no activity with guanine37 in yeast tRNAAsp(GUC) possessing a C36G37 sequence, guanine37 in Haloferax volcanii tRNAGlu(UUC) possessing a C36G37 sequence, guanine37 in yeast tRNAPhe A36U mutant(GAU) possessing a U36G37 sequence, guanine37 in Escherichia coli tRNASer(UGA) possessing a A36G37 sequence Aquifex aeolicus ?
-
 ?
2.1.1.228 S-adenosyl-L-methionine + guanine37 in Aquifex aeolicus tRNAArg(ACG)
-
 Aquifex aeolicus S-adenosyl-L-homocysteine + N1-methylguanine37 in Aquifex aeolicus tRNAArg(ACG)
-
 ?
2.1.1.228 S-adenosyl-L-methionine + guanine37 in Aquifex aeolicus tRNAArg(CCG)
-
 Aquifex aeolicus S-adenosyl-L-homocysteine + N1-methylguanine37 in Aquifex aeolicus tRNAArg(CCG)
-
 ?
2.1.1.228 S-adenosyl-L-methionine + guanine37 in Aquifex aeolicus tRNAGln(UUG)
-
 Aquifex aeolicus S-adenosyl-L-homocysteine + N1-methylguanine37 in Aquifex aeolicus tRNAGln(UUG)
-
 ?
2.1.1.228 S-adenosyl-L-methionine + guanine37 in Aquifex aeolicus tRNAHis(GUG)
-
 Aquifex aeolicus S-adenosyl-L-homocysteine + N1-methylguanine37 in Aquifex aeolicus tRNAHis(GUG)
-
 ?
2.1.1.228 S-adenosyl-L-methionine + guanine37 in Aquifex aeolicus tRNALeu(CAG)
-
 Aquifex aeolicus S-adenosyl-L-homocysteine + N1-methylguanine37 in Aquifex aeolicus tRNALeu(CAG)
-
 ?
2.1.1.228 S-adenosyl-L-methionine + guanine37 in Aquifex aeolicus tRNAPro(GGG)
-
 Aquifex aeolicus S-adenosyl-L-homocysteine + N1-methylguanine37 in Aquifex aeolicus tRNAPro(GGG)
-
 ?
2.1.1.228 S-adenosyl-L-methionine + guanine37 in Haloferax volcanii tRNACys(GCA)
-
 Aquifex aeolicus S-adenosyl-L-homocysteine + N1-methylguanine37 in Haloferax volcanii tRNACys(GCA)
-
 ?
2.1.1.228 S-adenosyl-L-methionine + guanine37 in Haloferax volcanii tRNALeu(CAA)
-
 Aquifex aeolicus S-adenosyl-L-homocysteine + N1-methylguanine37 in Haloferax volcanii tRNALeu(CAA)
-
 ?
2.1.1.228 S-adenosyl-L-methionine + guanine37 in Haloferax volcanii tRNATrp(CCA)
-
 Aquifex aeolicus S-adenosyl-L-homocysteine + N1-methylguanine37 in Haloferax volcanii tRNATrp(CCA)
-
 ?
2.1.1.228 S-adenosyl-L-methionine + guanine37 in Haloferax volcanii tRNATyr(GUA)
-
 Aquifex aeolicus S-adenosyl-L-homocysteine + N1-methylguanine37 in Haloferax volcanii tRNATyr(GUA)
-
 ?
2.1.1.228 S-adenosyl-L-methionine + guanine37 in in Escherichia coli tRNALeu(CAG)
-
 Aquifex aeolicus S-adenosyl-L-homocysteine + N1-methylguanine37 in in Escherichia coli tRNALeu(CAG)
-
 ?
2.1.1.228 S-adenosyl-L-methionine + guanine37 in tRNA
-
 Aquifex aeolicus S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA
-
 ?
2.1.1.228 S-adenosyl-L-methionine + guanine37 in tRNA the enzyme methylates tRNA transcripts possessing an A36G37 sequence as well as tRNA transcripts possessing a G36G37 sequence. tRNA transcripts possessing pyrimidine36G37 are not methylated at all. The modified nucleoside and the position in yeast tRNA(Phe) transcript are confirmed by LC/MS. Nine truncated tRNA molecules are tested to clarify the additional recognition site. The TrmD protein efficiently methylates the micro helix corresponding to the anti-codon arm. Because the disruption of the anti-codon stem causes the complete loss of the methyl group acceptance activity, the anti-codon stem is essential for the recognition. The existence of the D-arm structure inhibits the activity Aquifex aeolicus S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA
-
 ?
2.1.1.228 S-adenosyl-L-methionine + guanine37 in yeast tRNAPhe(GAA)
-
 Aquifex aeolicus S-adenosyl-L-homocysteine + N1-methylguanine37 in yeast tRNAPhe(GAA)
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.1.1.228 transfer RNA (m1G37) methyltransferase
-
 Aquifex aeolicus
2.1.1.228 TrmD
-
 Aquifex aeolicus
2.1.1.228 tRNA (m1G37) methyltransferase
-
 Aquifex aeolicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.1.228 60
-
 assay at Aquifex aeolicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.228 7.6
-
 assay at Aquifex aeolicus