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Literature summary extracted from

  • Hibi, M.; Sonoki, T.; Mori, H.
    Functional coupling between vanillate-O-demethylase and formaldehyde detoxification pathway (2005), FEMS Microbiol. Lett., 253, 237-242.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.13.82 genes vanA and vanB, DNA and amino acid sequence determination and analysis, functional coexpression of genes vanA and vanB in Escherichia coli K12 strain WV1181 Pseudomonas putida

Protein Variants

EC Number Protein Variants Comment Organism
1.14.13.82 additional information the reaction of the recombinant enzyme is one of the limiting steps of the prospective bioconversion from lignin-derived compounds to valuable products in recombinant Escherichia coli Pseudomonas putida

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.13.82 vanillic acid + NAD(P)H + H+ + O2 Pseudomonas putida functional coupling between vanillate-O-demethylase and formaldehyde detoxification pathway, formaldehyde is further converted to formate by glutathione-dependent formaldehyde dehydrogenase, encoded by gene frmA protocatechuic acid + NAD(P)+ + H2O + formaldehyde procatechuic acid is 3,4-dihydroxybenzoate ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.82 Pseudomonas putida
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.13.82 0.003 0.88 recombinant whole cell activities with both cofactors NADPH or NADH Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.82 vanillic acid + NAD(P)H + H+ + O2
-
 Pseudomonas putida protocatechuic acid + NAD(P)+ + H2O + formaldehyde procatechuic acid is 3,4-dihydroxybenzoate ?
1.14.13.82 vanillic acid + NAD(P)H + H+ + O2 functional coupling between vanillate-O-demethylase and formaldehyde detoxification pathway, formaldehyde is further converted to formate by glutathione-dependent formaldehyde dehydrogenase, encoded by gene frmA Pseudomonas putida protocatechuic acid + NAD(P)+ + H2O + formaldehyde procatechuic acid is 3,4-dihydroxybenzoate ?

Synonyms

EC Number Synonyms Comment Organism
1.14.13.82 vanillate-O-demethylase
-
 Pseudomonas putida

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.13.82 30
-
 assay at Pseudomonas putida

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.82 NAD(P)H
-
 Pseudomonas putida