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Literature summary extracted from

  • Urig, S.; Lieske, J.; Fritz-Wolf, K.; Irmler, A.; Becker, K.
    Truncated mutants of human thioredoxin reductase 1 do not exhibit glutathione reductase activity (2006), FEBS Lett., 580, 3595-3600.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.8.1.9 expressed in Escherichia coli Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
1.8.1.9 TrxR-16 truncated form of TrxR missing the last 16 C-terminal amino acids, without thioredoxin-reducing activity Homo sapiens
1.8.1.9 TrxR-16 K29R without thioredoxin-reducing activity Homo sapiens
1.8.1.9 TrxR-16 K29R/H108Y without thioredoxin-reducing activity Homo sapiens
1.8.1.9 TrxR-16 K29R/H108Y/A119N/V478E without thioredoxin-reducing activity Homo sapiens
1.8.1.9 U498C 1.4fold higher GSSG-reducing activity compared to the TrxR-16 enzyme Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.8.1.9 0.088
-
 NADPH wild type enzyme Homo sapiens
1.8.1.9 0.088
-
 5,5'-dithiobis(2-nitrobenzoic acid) wild type enzyme Homo sapiens
1.8.1.9 0.7
-
 NADPH mutant U498C Homo sapiens
1.8.1.9 0.7
-
 5,5'-dithiobis(2-nitrobenzoic acid) mutant U498C Homo sapiens
1.8.1.9 1.1
-
 NADPH TrxR-16 mutant K29R/H108Y/A119N/V478E Homo sapiens
1.8.1.9 1.1
-
 5,5'-dithiobis(2-nitrobenzoic acid) TrxR-16 mutant K29R/H108Y/A119N/V478E Homo sapiens
1.8.1.9 2.4
-
 NADPH TrxR-16, TrxR lacking the last 16 C-terminal amino acids Homo sapiens
1.8.1.9 2.4
-
 5,5'-dithiobis(2-nitrobenzoic acid) TrxR-16, TrxR lacking the last 16 C-terminal amino acids Homo sapiens
1.8.1.9 3.3
-
 NADPH TrxR-16 mutant K29R Homo sapiens
1.8.1.9 3.3
-
 5,5'-dithiobis(2-nitrobenzoic acid) TrxR-16 mutant K29R Homo sapiens
1.8.1.9 4.5
-
 NADPH TrxR-16 mutant K29R/H108Y Homo sapiens
1.8.1.9 4.5
-
 5,5'-dithiobis(2-nitrobenzoic acid) TrxR-16 mutant K29R/H108Y Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
1.8.1.9 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.8.1.9 Ni-NTA resin affinity chromatography Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.8.1.9 placenta
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.8.1.9 0.003
-
 TrxR-16 mutant K29R/H108Y/A119N/V478E Homo sapiens
1.8.1.9 0.007
-
 TrxR-16 mutant K29R/H108Y Homo sapiens
1.8.1.9 0.016
-
 TrxR-16 mutant K29R Homo sapiens
1.8.1.9 0.025
-
 TrxR-16, TrxR lacking the last 16 C-terminal amino acids Homo sapiens
1.8.1.9 0.035
-
 mutant U498C Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.1.9 5,5'-dithiobis(2-nitrobenzoic acid) + NADPH
-
 Homo sapiens 2-nitro-5-thiobenzoate + NADP+
-
 ?
1.8.1.9 GSSG + NADPH
-
 Homo sapiens GSH + NADP+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.8.1.9 thioredoxin reductase 1
-
 Homo sapiens
1.8.1.9 TrxR1
-
 Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.8.1.9 0.012
-
 5,5'-dithiobis(2-nitrobenzoic acid) mutant U498C Homo sapiens
1.8.1.9 0.018
-
 5,5'-dithiobis(2-nitrobenzoic acid) TrxR-16 mutant K29R/H108Y/A119N/V478E Homo sapiens
1.8.1.9 0.075
-
 5,5'-dithiobis(2-nitrobenzoic acid) TrxR-16 mutant K29R/H108Y Homo sapiens
1.8.1.9 0.52
-
 5,5'-dithiobis(2-nitrobenzoic acid) TrxR-16 mutant K29R Homo sapiens
1.8.1.9 0.55
-
 5,5'-dithiobis(2-nitrobenzoic acid) TrxR-16, TrxR lacking the last 16 C-terminal amino acids Homo sapiens
1.8.1.9 33.33
-
 5,5'-dithiobis(2-nitrobenzoic acid) wild type enzyme Homo sapiens