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Literature summary extracted from

  • Mukougawa, K.; Kanamoto, H.; Kobayashi, T.; Yokota, A.; Kohchi, T.
    Metabolic engineering to produce phytochromes with phytochromobilin, phycocyanobilin, or phycoerythrobilin chromophore in Escherichia coli (2006), FEBS Lett., 580, 1333-1338.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.3.7.3 additional information metabolic engineering of bacteria for the production of various bilins for assembly into phytochromes will facilitate the molecular analysis of photoreceptors Synechococcus sp.
1.3.7.5 additional information co-expression of heme oxygenase in a single operon in conjunction with apophytochrome, is a system to produce phytochromes with various chromophores in Escherichia coli, metabolic engineering of bacteria for the production of various bilins for assembly into phytochromes will facilitate the molecular analysis of photoreceptors Synechococcus sp.

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.7.3 into vector pKT270, co-expression with cyanobacterial phytochrome 1 in Escherichia coli JM109 Synechococcus sp.
1.3.7.4 development of a system to produce phytochromobilin in Escherichia coli by coexpression of heme oxygenase and phytochromobilin:ferredoxin oxidoreductase in a single operon in conjunction with apophytochrome using two compatible plasmids Escherichia coli
1.3.7.5 subcloned into pKT210, co-expression with heme oxygenase in Escherichia coli cells Synechococcus sp.

Organism

EC Number Organism UniProt Comment Textmining
1.3.7.3 Synechococcus sp.
-
-
-
1.3.7.3 Synechococcus sp. W8020
-
-
-
1.3.7.4 Escherichia coli
-
-
-
1.3.7.5 Synechococcus sp.
-
-
-
1.3.7.5 Synechococcus sp. W8020
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.7.3 15,16-dihydrobiliverdin + reduced ferredoxin
-
 Synechococcus sp. (3Z)-phycoerythrobilin + oxidized ferredoxin
-
 ?
1.3.7.3 15,16-dihydrobiliverdin + reduced ferredoxin
-
 Synechococcus sp. W8020 (3Z)-phycoerythrobilin + oxidized ferredoxin
-
 ?
1.3.7.5 biliverdin IXa + reduced ferredoxin
-
 Synechococcus sp. (3Z)-phycocyanobilin + oxidized ferredoxin
-
 ?
1.3.7.5 biliverdin IXa + reduced ferredoxin
-
 Synechococcus sp. W8020 (3Z)-phycocyanobilin + oxidized ferredoxin
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.3.7.3 PebB
-
 Synechococcus sp.
1.3.7.5 PcyA
-
 Synechococcus sp.