Literature summary extracted from

Wolthers, K.R.; Toogood, H.S.; Jowitt, T.A.; Marshall, K.R.; Leys, D.; Scrutton, N.S.
Crystal structure and solution characterization of the activation domain of human methionine synthase (2007), FEBS J., 274, 738-750.

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
2.1.1.13	expressed in Escherichia coli BL21(DE3) cells	Homo sapiens

Crystallization (Commentary)

EC Number	Crystallization (Comment)	Organism
2.1.1.13	sitting drop vapour diffusion method in reservoir solution of 0.1 M Tris/HCl pH 7.5 containing 0.1-0.4 M sodium acetate, 10-12% poly(ethylene glycol) 8000 and 10-12% poly(ethyleneglycol) 1000	Homo sapiens

Protein Variants

EC Number	Protein Variants	Comment	Organism
2.1.1.13	D963E /K1071N	reduced activity	Homo sapiens

Molecular Weight [Da]

EC Number	Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
2.1.1.13	36200	-	sedimentation in high salt buffer	Homo sapiens
2.1.1.13	40000	-	2 * 40000, SDS-PAGE	Homo sapiens
2.1.1.13	43100	-	1 * 43100, light scattering	Homo sapiens

Organism

EC Number	Organism	UniProt	Comment	Textmining
2.1.1.13	Homo sapiens	-	-	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
2.1.1.13	Ni-NTA resin HisTrap column chromatography, HiPrep Q Sepharose column chromatography, and Resource Q column chromatography	Homo sapiens

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2.1.1.13	N5-methyltetrahydropteroylmonoglutamate + L-homocysteine	-	Homo sapiens	tetrahydropteroylmonoglutamate + L-methionine	-	?

Subunits

EC Number	Subunits	Comment	Organism
2.1.1.13	dimer	2 * 40000, SDS-PAGE	Homo sapiens
2.1.1.13	monomer	1 * 43100, light scattering	Homo sapiens

Synonyms

EC Number	Synonyms	Comment	Organism
2.1.1.13	5-methyltetrahydrofolate homocysteine methyltransferase	-	Homo sapiens

Cofactor

EC Number	Cofactor	Comment	Organism	Structure
2.1.1.13	vitamin B12	dependent	Homo sapiens

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Release 2023.1 (January 2023)