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Literature summary extracted from

  • Ma, S.M.; Tang, Y.
    Biochemical characterization of the minimal polyketide synthase domains in the lovastatin nonaketide synthase LovB (2007), FEBS J., 274, 2854-2864.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.161 minimal polyketide synthase domains of subunit LovB as standalone proteins Aspergillus terreus
2.3.1.161 the entire lovB gene is inserted into pET21a+ to create pSMa33, the LovB KS-MAT didomain, MAT, ACP, ACP-CON and CON are each amplified from pSMa33 and cloned into pET28a+ Aspergillus terreus

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.161 additional information expression of minimal polyketide synthase domains of subunit LovB as standalone proteins. The didomain proteins ketosynthase-malonyl-coenzyme A:acyl carrier protein acyltransferase KS-MAT can transfer the acyl group to both the cognate LovB acyl carrier protein and heterologous acyl carrier proteins from bacterial type I and type II polyketide synthases. It transfers malonyl and acetyl groups with kcat/Km values of 0.62 per min and 0.032 per min, resp Aspergillus terreus
2.3.1.161 S208A LovB MAT mutant Aspergillus terreus
2.3.1.161 S209A LovB MAT mutant Aspergillus terreus
2.3.1.161 S656A inactivation of malonyl-coenzyme A:acyl carrier protein acyltransferase domain in ketosynthase-malonyl-coenzyme A:acyl carrier protein acyltransferase for assay of ketosynthase activity. Ketosynthase displays no activity towards acetyl groups but recognizes malonyl groups in the absence of cerulenin Aspergillus terreus
2.3.1.161 S656A LovB KS-MAT mutant, MAT domain inactivated Aspergillus terreus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.161 11000
-
 LovB ACP, determined by SDS-PAGE Aspergillus terreus
2.3.1.161 52000
-
 LovB MAT, determined by SDS-PAGE Aspergillus terreus
2.3.1.161 66000
-
 LovB ACP-CON, determined by SDS-PAGE Aspergillus terreus
2.3.1.161 103000
-
 LovB KS-MAT, determined by SDS-PAGE Aspergillus terreus
2.3.1.161 335000
-
 LovB Aspergillus terreus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.161 acetyl-CoA + malonyl-CoA + NADPH + H+ + S-adenosyl-L-methionine Aspergillus terreus
-
 dihydromonacolin L + CoA + CO2 + NADP+ + S-adenosyl-L-homocysteine + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.161 Aspergillus terreus
-
-
-
2.3.1.161 Aspergillus terreus Q9Y8A5
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.161 the single domains are purified using Ni-nitrilotriacetic agarose resin and anion-exchange chromatography Aspergillus terreus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.161 acetyl-CoA + malonyl-CoA + NADPH + H+ + S-adenosyl-L-methionine
-
 Aspergillus terreus dihydromonacolin L + CoA + CO2 + NADP+ + S-adenosyl-L-homocysteine + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.161 lovastatin nonaketide synthase
-
 Aspergillus terreus
2.3.1.161 LovB
-
 Aspergillus terreus