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Literature summary extracted from

  • Jonsson, A.; Teixeira, P.F.; Nordlund, S.
    The activity of adenylyltransferase in Rhodospirillum rubrum is only affected by alpha-ketoglutarate and unmodified PII proteins, but not by glutamine, in vitro (2007), FEBS J., 274, 2449-2460.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.7.42 PII proteins PII proteins only stimulate the adenylylation activity of ATase Rhodospirillum rubrum

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.42 cloned in Escherichia coli Rhodospirillum rubrum

Protein Variants

EC Number Protein Variants Comment Organism
2.7.7.42 additional information sequences for the active sites for adenylylation and deadenylylation as in Escherichia coli are also present in Rhodospirillum rubrum, between amino acids 107-329 and 632-849, respectively, however a Q-linker as in Escherichia coli can not be identified Rhodospirillum rubrum
2.7.7.42 additional information the C-terminal part of the protein between amino acids 1006-1149 shows high similarity to an Escherichia coli bacterioferritin comigratory protein (BCP) Rhodospirillum rubrum

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.42 alpha-ketoglutarate in Rhodospirillum rubrum alpha-ketoglutarate inhibits the adenylylation activity of ATase Rhodospirillum rubrum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.42 K2HPO4 deadenylylation assay with 10mM K2HPO4 Rhodospirillum rubrum
2.7.7.42 Mg2+ adenylylation assay with 10mM Mg2+ Rhodospirillum rubrum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.42 126200
-
 estimated from gene sequence Rhodospirillum rubrum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.42 glutamine synthase + ATP Rhodospirillum rubrum ATase primarily regulated by alpha-ketoglutarate, glutamine has no effect on neither the adenylylation nor the deadenylylation of glutamine synthetase, PII proteins only stimulate the adenylylation reaction adenylyl-[glutamine synthase] + diphosphate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.42 Rhodospirillum rubrum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.42 ATase is cloned into the pET101/D-TOPO plasmid, containing ATase fused to a C-terminal V5 epitope followed by six histidines, His-tagged proteins are applied to HisTrap HP 1ml column Rhodospirillum rubrum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.42 glutamine synthase + ATP ATase primarily regulated by alpha-ketoglutarate, glutamine has no effect on neither the adenylylation nor the deadenylylation of glutamine synthetase, PII proteins only stimulate the adenylylation reaction Rhodospirillum rubrum adenylyl-[glutamine synthase] + diphosphate
-
 r

Synonyms

EC Number Synonyms Comment Organism
2.7.7.42 adenylyltransferase
-
 Rhodospirillum rubrum
2.7.7.42 ATASE
-
 Rhodospirillum rubrum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.42 7.6
-
 assay at Rhodospirillum rubrum