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Literature summary extracted from
- Functional characterization of front-end desaturases from trypanosomatids depicts the first polyunsaturated fatty acid biosynthetic pathway from a parasitic protozoan (2006), FEBS J., 273, 271-280.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.14.19.4 | expression in Saccharomyces cerevisiae | Leishmania major |
| 1.14.19.31 | DNA and amino acid sequence determination and analysis, sequence comparison, phylogenetic analysis, recombinant functional expression in Saccharomyces cerevisiae strain HH3 | Leishmania major |
| 1.14.19.31 | DNA and amino acid sequence determination and analysis, sequence comparison, phylogenetic analysis, recombinant functional expression in Saccharomyces cerevisiae strain HH3 | Trypanosoma cruzi |
| 1.14.19.31 | DNA and amino acid sequence determination and analysis, sequence comparison, phylogenetic analysis, recombinant functional expression in Saccharomyces cerevisiae strain HH3 | Trypanosoma brucei |
| 1.14.19.44 | expressed in Saccharomyces cerevisiae strain HH3 | Leishmania major |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 1.14.19.31 | kinetoplast | - |
Leishmania major | 20023 | - |
| 1.14.19.31 | kinetoplast | - |
Trypanosoma cruzi | 20023 | - |
| 1.14.19.31 | kinetoplast | - |
Trypanosoma brucei | 20023 | - |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.19.44 | (8Z,11Z,14Z)-icosa-8,11,14-trienoyl-CoA + ferrocytochrome b5 + O2 + H+ | Leishmania major | - |
arachidonoyl-CoA + ferricytochrome b5 + H2O | - |
? |  |
| 1.14.19.44 | (8Z,11Z,14Z)-icosa-8,11,14-trienoyl-CoA + ferrocytochrome b5 + O2 + H+ | Leishmania major Friedlin | - |
arachidonoyl-CoA + ferricytochrome b5 + H2O | - |
? | |
| 1.14.19.44 | (8Z,11Z,14Z,17Z)-icosa-8,11,14,17-tetraenoyl-CoA + ferrocytochrome b5 + O2 + H+ | Leishmania major | - |
(5Z,8Z,11Z,14Z,17Z)-icosa-5,8,11,14,17-pentaenoyl-CoA + ferricytochrome b5 + H2O | - |
? | |
| 1.14.19.44 | (8Z,11Z,14Z,17Z)-icosa-8,11,14,17-tetraenoyl-CoA + ferrocytochrome b5 + O2 + H+ | Leishmania major Friedlin | - |
(5Z,8Z,11Z,14Z,17Z)-icosa-5,8,11,14,17-pentaenoyl-CoA + ferricytochrome b5 + H2O | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.19.4 | Leishmania major | - |
- |
- |
| 1.14.19.31 | Leishmania major | Q4QFK0 | gene LMJF_14_1340 | - |
| 1.14.19.31 | Leishmania major Friedlin | Q4QFK0 | gene LMJF_14_1340 | - |
| 1.14.19.31 | Trypanosoma brucei | Q38AQ3 | can be classified as DELTA4 desaturase due to its HX3HH motif; gene Tb10.6k15.3610 | - |
| 1.14.19.31 | Trypanosoma brucei 427 | Q38AQ3 | can be classified as DELTA4 desaturase due to its HX3HH motif; gene Tb10.6k15.3610 | - |
| 1.14.19.31 | Trypanosoma cruzi | Q4DDJ1 | can be classified as DELTA4 desaturase due to its HX3HH motif; gene Tc00.1047053510181.20 | - |
| 1.14.19.31 | Trypanosoma cruzi CL Brener | Q4DDJ1 | can be classified as DELTA4 desaturase due to its HX3HH motif; gene Tc00.1047053510181.20 | - |
| 1.14.19.44 | Leishmania major | - |
- |
- |
| 1.14.19.44 | Leishmania major Friedlin | - |
- |
- |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.19.31 | 22:4 DELTA7,10,13,16-docosatetraenoic acid + 2 ferrocytochrome b5 + O2 + 2 H+ | - |
Trypanosoma brucei | 22:5 DELTA4,7,10,13,16-docosapentaenoic acid + 2 ferricytochrome b5 + 2 H2O | - |
? | |
| 1.14.19.31 | 22:4 DELTA7,10,13,16-docosatetraenoic acid + 2 ferrocytochrome b5 + O2 + 2 H+ | - |
Trypanosoma brucei 427 | 22:5 DELTA4,7,10,13,16-docosapentaenoic acid + 2 ferricytochrome b5 + 2 H2O | - |
? | |
| 1.14.19.31 | 22:5 DELTA7,10,13,16,19-docosapentaenoic acid + 2 ferrocytochrome b5 + O2 + 2 H+ | - |
Trypanosoma brucei | 22:6 DELTA4,7,10,13,16,19-docosahexaenoic acid + 2 ferricytochrome b5 + 2 H2O | - |
? | |
| 1.14.19.31 | 22:5 DELTA7,10,13,16,19-docosapentaenoic acid + 2 ferrocytochrome b5 + O2 + 2 H+ | - |
Trypanosoma brucei 427 | 22:6 DELTA4,7,10,13,16,19-docosahexaenoic acid + 2 ferricytochrome b5 + 2 H2O | - |
? | |
| 1.14.19.31 | docosa-7,10,13,16,19-pentaenoic acid + 2 ferrocytochrome b5 + O2 + 2 H+ | - |
Leishmania major | docosa-4,7,10,13,16,19-hexaenoic acid + 2 ferricytochrome b5 + 2 H2O | - |
? | |
| 1.14.19.31 | docosa-7,10,13,16,19-pentaenoic acid + 2 ferrocytochrome b5 + O2 + 2 H+ | - |
Trypanosoma cruzi | docosa-4,7,10,13,16,19-hexaenoic acid + 2 ferricytochrome b5 + 2 H2O | - |
? | |
| 1.14.19.31 | docosa-7,10,13,16,19-pentaenoic acid + 2 ferrocytochrome b5 + O2 + 2 H+ | - |
Trypanosoma cruzi CL Brener | docosa-4,7,10,13,16,19-hexaenoic acid + 2 ferricytochrome b5 + 2 H2O | - |
? | |
| 1.14.19.31 | docosa-7,10,13,16,19-pentaenoic acid + 2 ferrocytochrome b5 + O2 + 2 H+ | - |
Leishmania major Friedlin | docosa-4,7,10,13,16,19-hexaenoic acid + 2 ferricytochrome b5 + 2 H2O | - |
? | |
| 1.14.19.31 | docosa-7,10,13,16-tetraenoic acid + 2 ferrocytochrome b5 + O2 + 2 H+ | - |
Leishmania major | docosa-4,7,10,13,16-pentaenoic acid + 2 ferricytochrome b5 + 2 H2O | - |
? | |
| 1.14.19.31 | docosa-7,10,13,16-tetraenoic acid + 2 ferrocytochrome b5 + O2 + 2 H+ | - |
Trypanosoma cruzi | docosa-4,7,10,13,16-pentaenoic acid + 2 ferricytochrome b5 + 2 H2O | - |
? | |
| 1.14.19.31 | docosa-7,10,13,16-tetraenoic acid + 2 ferrocytochrome b5 + O2 + 2 H+ | - |
Trypanosoma cruzi CL Brener | docosa-4,7,10,13,16-pentaenoic acid + 2 ferricytochrome b5 + 2 H2O | - |
? | |
| 1.14.19.31 | docosa-7,10,13,16-tetraenoic acid + 2 ferrocytochrome b5 + O2 + 2 H+ | - |
Leishmania major Friedlin | docosa-4,7,10,13,16-pentaenoic acid + 2 ferricytochrome b5 + 2 H2O | - |
? | |
| 1.14.19.44 | (8Z,11Z,14Z)-icosa-8,11,14-trienoyl-CoA + ferrocytochrome b5 + O2 + H+ | - |
Leishmania major | arachidonoyl-CoA + ferricytochrome b5 + H2O | - |
? | |
| 1.14.19.44 | (8Z,11Z,14Z)-icosa-8,11,14-trienoyl-CoA + ferrocytochrome b5 + O2 + H+ | - |
Leishmania major Friedlin | arachidonoyl-CoA + ferricytochrome b5 + H2O | - |
? | |
| 1.14.19.44 | (8Z,11Z,14Z,17Z)-icosa-8,11,14,17-tetraenoyl-CoA + ferrocytochrome b5 + O2 + H+ | - |
Leishmania major | (5Z,8Z,11Z,14Z,17Z)-icosa-5,8,11,14,17-pentaenoyl-CoA + ferricytochrome b5 + H2O | - |
? | |
| 1.14.19.44 | (8Z,11Z,14Z,17Z)-icosa-8,11,14,17-tetraenoyl-CoA + ferrocytochrome b5 + O2 + H+ | - |
Leishmania major Friedlin | (5Z,8Z,11Z,14Z,17Z)-icosa-5,8,11,14,17-pentaenoyl-CoA + ferricytochrome b5 + H2O | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.14.19.4 | DELTA8 desaturase | - |
Leishmania major |
| 1.14.19.31 | DELTA4 desaturase | - |
Leishmania major |
| 1.14.19.31 | DELTA4 desaturase | - |
Trypanosoma cruzi |
| 1.14.19.31 | DELTA4 desaturase | - |
Trypanosoma brucei |
| 1.14.19.44 | DELTA 5-desaturase | - |
Leishmania major |
| 1.14.19.44 | FADDELTA5 | - |
Leishmania major |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.19.31 | cytochrome b5 | - |
Leishmania major | |
| 1.14.19.31 | cytochrome b5 | - |
Trypanosoma cruzi | |
| 1.14.19.31 | cytochrome b5 | - |
Trypanosoma brucei | |
| 1.14.19.44 | cytochrome b5 | - |
Leishmania major |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 1.14.19.31 | evolution | the enzyme belongs to the front-end desaturases, phylogenetic analysis, overview. The HX3HH motif can be used to a priori characterize the enzyme as front-end DELTA4 desaturase | Leishmania major |
| 1.14.19.31 | evolution | the enzyme belongs to the front-end desaturases, phylogenetic analysis, overview. The HX3HH motif can be used to a priori characterize the enzyme as front-end DELTA4 desaturase | Trypanosoma cruzi |
| 1.14.19.31 | evolution | the enzyme belongs to the front-end desaturases, phylogenetic analysis, overview. The HX3HH motif can be used to a priori characterize the enzyme as front-end DELTA4 desaturase | Trypanosoma brucei |
| 1.14.19.31 | metabolism | the enzyme is involved in the pathway for very-long-chain polyunsaturated fatty acid biosynthesis existing in the protozoan. The enzymes involved in polyunsaturated fatty acid biosynthesis (i.e. DELTA6, DELTA8, DELTA5 and DELTA4 desaturases) are named front-end desaturases, because they introduce a double bond between a pre-existent olefinic bond and the carboxyl end of the fatty acid molecule. A complete pathway for very-long-chain polyunsaturated fatty acid biosynthesis is functional in Leishmania major using DELTA6, DELTA5 and DELTA4 desaturases | Leishmania major |
| 1.14.19.31 | metabolism | the enzymes involved in polyunsaturated fatty acid biosynthesis (i.e. DELTA6, DELTA8, DELTA5 and DELTA4 desaturases) are named front-end desaturases, because they introduce a double bond between a pre-existent olefinic bond and the carboxyl end of the fatty acid molecule. In trypanosomes, only DELTA4-desaturases are present from a complete pathway for very-long-chain polyunsaturated fatty acid biosynthesis | Trypanosoma cruzi |
| 1.14.19.31 | metabolism | the enzymes involved in polyunsaturated fatty acid biosynthesis (i.e. DELTA6, DELTA8, DELTA5 and DELTA4 desaturases) are named front-end desaturases, because they introduce a double bond between a pre-existent olefinic bond and the carboxyl end of the fatty acid molecule. In trypanosomes, only DELTA4-desaturases are present from a complete pathway for very-long-chain polyunsaturated fatty acid biosynthesis, the enzyme is also able to use n-3 and n-6 fatty acid isomers | Trypanosoma brucei |
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