EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.12.7.2 | C42S | mutant enzyme with almost no activity in the reaction with H2 and oxidized benzyl viologen | Methanosarcina barkeri |
1.12.7.2 | C45S | mutants with about 5% of the wild-type activity in the reaction with H2 and oxidized benzyl viologen | Methanosarcina barkeri |
1.12.7.2 | C48S | mutant enzyme with almost no activity in the reaction with H2 and oxidized benzyl viologen | Methanosarcina barkeri |
1.12.7.2 | C73S | mutants with about 5% of the wild-type activity in the reaction with H2 and oxidized benzyl viologen | Methanosarcina barkeri |
1.12.7.2 | C76S | mutant enzyme with almost no activity in the reaction with H2 and oxidized benzyl viologen | Methanosarcina barkeri |
1.12.7.2 | C79S | mutants with about 5% of the wild-type activity in the reaction with H2 and oxidized benzyl viologen | Methanosarcina barkeri |
1.12.7.2 | C83S | mutant enzyme with approximately 10% of the activity of the wild-type enzyme in the reaction with H2 and oxidized benzyl viologen | Methanosarcina barkeri |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.12.7.2 | membrane | bound to | Methanosarcina barkeri | 16020 | - |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.12.7.2 | Methanosarcina barkeri | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.12.7.2 | - |
Methanosarcina barkeri |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.12.7.2 | H2 + ferredoxin + oxidized metronidazole | - |
Methanosarcina barkeri | H+ + ferredoxin + reduced metronidazole | - |
? | |
1.12.7.2 | H2 + oxidized benzyl viologen | wild-type enzyme catalysed the reduction of benzylviologen at fourfold higher rates than the reduction of ferredoxin | Methanosarcina barkeri | reduced benzyl viologen + H+ | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.12.7.2 | Ech hydrogenase | - |
Methanosarcina barkeri |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.12.7.2 | iron-sulfur centre | the three [4Fe-4S] clusters simultaneously mediate electron and proton transfer and thus could be an essential part of the proton-translocating machinery | Methanosarcina barkeri |