EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
2.7.1.165 | additional information | 50 mM NaN3, 10 mM phenylmethyl sulfonylfluoride, and H2O2 have no obvious effect on the enzymatic activity | Pyrococcus horikoshii |
EC Number | Cloned (Comment) | Organism |
---|---|---|
2.7.1.165 | - |
Pyrococcus horikoshii |
2.7.1.165 | expressed in Escherichia coli | Pyrococcus horikoshii |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.7.1.165 | CuCl2 | 1 mM, 61% inhibition; 61% inhibition at 1 mM | Pyrococcus horikoshii | |
2.7.1.165 | HgCl2 | 11% inhibition at 1 mM; 1 mM, 11% inhibition | Pyrococcus horikoshii | |
2.7.1.165 | additional information | resistant to the urea, ethanol, 2-propanol, n-butanol and DMSO (10% v/v each) | Pyrococcus horikoshii |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.1.165 | 0.044 | - |
D-glycerate | - |
Pyrococcus horikoshii | |
2.7.1.165 | 0.044 | - |
(R)-glycerate | the reaction mixture contains 1 mM (R)-glycerate, 1 mM ATP, 10 mM MgCl2, 0.3 mM NADH, 1 mM phosphoenolpyruvate, 10 nM purified glycerate kinase, 3.5 units pyruvate kinase and 5 units lactate dehydrogenase in 50 mM potassium phosphate (pH 7.0), at 45°C | Pyrococcus horikoshii | |
2.7.1.165 | 0.102 | - |
ATP | - |
Pyrococcus horikoshii | |
2.7.1.165 | 0.102 | - |
ATP | the reaction mixture contains 1 mM (R)-glycerate, 1 mM ATP, 10 mM MgCl2, 0.3 mM NADH, 1 mM phosphoenolpyruvate, 10 nM purified glycerate kinase, 3.5 units pyruvate kinase and 5 units lactate dehydrogenase in 50 mM potassium phosphate (pH 7.0), at 45°C | Pyrococcus horikoshii |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.7.1.165 | Co2+ | 10 mM, 68% of the activity with Mg2+ | Pyrococcus horikoshii | |
2.7.1.165 | K+ | 7.94fold increase of activity at 50 mM | Pyrococcus horikoshii | |
2.7.1.165 | K+ | 50 mM, 7.9fold activation | Pyrococcus horikoshii | |
2.7.1.165 | Li+ | 2.28fold increase of activity at 50 mM | Pyrococcus horikoshii | |
2.7.1.165 | Li+ | 50 mM, 2.3fold activation | Pyrococcus horikoshii | |
2.7.1.165 | Mg2+ | 10 mM, required for activity | Pyrococcus horikoshii | |
2.7.1.165 | Mg2+ | no activity is observed in the absence of divalent metal ion and maximal activity is observed in the presence of Mg2+ (10 mM) | Pyrococcus horikoshii | |
2.7.1.165 | Mn2+ | 10 mM, 76% of the activity with Mg2+ | Pyrococcus horikoshii | |
2.7.1.165 | additional information | when Mn2+, Co2+, Ca2+, Sr2+ and Ni2+ is substituted for Mg2+, respectively, Mn2+, Co2+ and Ni2+ show 76, 68 and 11% activity of that for Mg2+ | Pyrococcus horikoshii | |
2.7.1.165 | Na+ | 3.49fold increase of activity at 50 mM | Pyrococcus horikoshii | |
2.7.1.165 | Na+ | 50 mM, 3.5fold activation | Pyrococcus horikoshii | |
2.7.1.165 | NH4+ | 7.83fold increase of activity at 50 mM | Pyrococcus horikoshii | |
2.7.1.165 | NH4+ | 50 mM, 7.8fold activation | Pyrococcus horikoshii | |
2.7.1.165 | Ni2+ | 10 mM, 11% of the activity with Mg2+ | Pyrococcus horikoshii |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.7.1.165 | 47000 | - |
SDS-PAGE | Pyrococcus horikoshii |
2.7.1.165 | 47000 | - |
2 * 47000, SDS-PAGE | Pyrococcus horikoshii |
2.7.1.165 | 47400 | - |
calculated from amino acid sequence | Pyrococcus horikoshii |
2.7.1.165 | 50000 | - |
2 * 50000, gel filtration | Pyrococcus horikoshii |
2.7.1.165 | 100000 | - |
gel filtration | Pyrococcus horikoshii |
EC Number | Organic Solvent | Comment | Organism |
---|---|---|---|
2.7.1.165 | 2-propanol | resistant to | Pyrococcus horikoshii |
2.7.1.165 | DMSO | resistant to | Pyrococcus horikoshii |
2.7.1.165 | Ethanol | resistant to | Pyrococcus horikoshii |
2.7.1.165 | n-Butanol | resistant to | Pyrococcus horikoshii |
2.7.1.165 | urea | resistant to | Pyrococcus horikoshii |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.1.165 | Pyrococcus horikoshii | - |
- |
- |
2.7.1.165 | Pyrococcus horikoshii | O58231 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.7.1.165 | nickel affinity chromatography, HiTrap Q ion exchange chromatography, and SephacrylTM S-200 HR gel filtration | Pyrococcus horikoshii |
EC Number | Storage Stability | Organism |
---|---|---|
2.7.1.165 | 90°C, 50 mM Tris-HCl and 100 mM NaCl buffer (pH 8.0), 12 h, almost no loss of activity | Pyrococcus horikoshii |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.1.165 | ADP + (R)-glycerate | at 32% of the activity with ATP | Pyrococcus horikoshii | AMP + 3-phospho-(R)-glycerate | - |
? | |
2.7.1.165 | ADP + (R)-glycerate | 32% of the activity with ATP | Pyrococcus horikoshii | AMP + 2-phospho-(R)-glycerate | - |
? | |
2.7.1.165 | ATP + (R)-glycerate | - |
Pyrococcus horikoshii | ADP + 3-phospho-(R)-glycerate | - |
? | |
2.7.1.165 | ATP + (R)-glycerate | - |
Pyrococcus horikoshii | ADP + 2-phospho-(R)-glycerate | - |
? | |
2.7.1.165 | ATP + D-glycerate | - |
Pyrococcus horikoshii | ADP + 2-phospho-D-glycerate | - |
? | |
2.7.1.165 | CTP + (R)-glycerate | at 73% of the activity with ATP | Pyrococcus horikoshii | CDP + 3-phospho-(R)-glycerate | - |
? | |
2.7.1.165 | CTP + (R)-glycerate | 73% of the activity with ATP | Pyrococcus horikoshii | CDP + 2-phospho-(R)-glycerate | - |
? | |
2.7.1.165 | diphosphate + (R)-glycerate | at 112% of the activity with ATP | Pyrococcus horikoshii | phosphate + 3-phospho-(R)-glycerate | - |
? | |
2.7.1.165 | diphosphate + (R)-glycerate | 112% of the activity with ATP | Pyrococcus horikoshii | phosphate + 2-phospho-(R)-glycerate | - |
? | |
2.7.1.165 | GTP + (R)-glycerate | at 64% of the activity with ATP | Pyrococcus horikoshii | GDP + 3-phospho-(R)-glycerate | - |
? | |
2.7.1.165 | GTP + (R)-glycerate | 64% of the activity with ATP | Pyrococcus horikoshii | GDP + 2-phospho-(R)-glycerate | - |
? | |
2.7.1.165 | additional information | AMP is not a substrate | Pyrococcus horikoshii | ? | - |
? | |
2.7.1.165 | UTP + (R)-glycerate | at 29% of the activity with ATP | Pyrococcus horikoshii | UDP + 3-phospho-(R)-glycerate | - |
? | |
2.7.1.165 | UTP + (R)-glycerate | 29% of the activity with ATP | Pyrococcus horikoshii | UDP + 2-phospho-(R)-glycerate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.7.1.165 | dimer | 2 * 47000, SDS-PAGE | Pyrococcus horikoshii |
2.7.1.165 | dimer | 2 * 50000, gel filtration | Pyrococcus horikoshii |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.7.1.165 | 2-PGA forming glycerate kinase | - |
Pyrococcus horikoshii |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.7.1.165 | 45 | - |
- |
Pyrococcus horikoshii |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.7.1.165 | 30 | 50 | strong activity at 30-50°C | Pyrococcus horikoshii |
2.7.1.165 | 45 | 100 | 45°C: optimum, 100°C: about 50% of maximal activity | Pyrococcus horikoshii |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.7.1.165 | 90 | - |
12 h, no loss of activity | Pyrococcus horikoshii |
2.7.1.165 | 100 | - |
about half of the maximal activity remains at 100°C | Pyrococcus horikoshii |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.1.165 | 360 | - |
(R)-glycerate | the reaction mixture contains 1 mM (R)-glycerate, 1 mM ATP, 10 mM MgCl2, 0.3 mM NADH, 1 mM phosphoenolpyruvate, 10 nM purified glycerate kinase, 3.5 units pyruvate kinase and 5 units lactate dehydrogenase in 50 mM potassium phosphate (pH 7.0), at 45°C | Pyrococcus horikoshii | |
2.7.1.165 | 489.2 | - |
D-glycerate | - |
Pyrococcus horikoshii | |
2.7.1.165 | 500.6 | - |
ATP | - |
Pyrococcus horikoshii |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.7.1.165 | 7 | - |
- |
Pyrococcus horikoshii |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
2.7.1.165 | 6 | 10 | 50% of maximal activity at pH 6.0 and 10.0 | Pyrococcus horikoshii |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
2.7.1.165 | 6 | 10 | half of the maximum activity remains at pH 6-10 | Pyrococcus horikoshii |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
2.7.1.165 | ATP | - |
Pyrococcus horikoshii |