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Literature summary extracted from

  • Goudarzi, M.; Born, T.L.
    Purification and characterization of Thermotoga maritima homoserine transsuccinylase indicates it is a transacetylase (2006), Extremophiles, 10, 469-478.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.31 expression in Escherichia coli Thermotoga maritima

General Stability

EC Number General Stability Organism
2.3.1.31 temperature inactivation readily reversible Thermotoga maritima

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.31 iodoacetamide
-
 Thermotoga maritima

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.31 0.017
-
 malonyl-CoA 25°C, pH 7.5 Thermotoga maritima
2.3.1.31 0.044
-
 propionyl-CoA 25°C, pH 7.5 Thermotoga maritima
2.3.1.31 0.051
-
 succinyl-CoA 25°C, pH 7.5 Thermotoga maritima
2.3.1.31 0.068
-
 isobutyryl-CoA 25°C, pH 7.5 Thermotoga maritima
2.3.1.31 0.083
-
 beta-hydroxybutyryl-CoA 25°C, pH 7.5 Thermotoga maritima
2.3.1.31 0.13
-
 acetyl-CoA 25°C, pH 7.5 Thermotoga maritima
2.3.1.31 0.14
-
 CoA 25°C, pH 7.5 Thermotoga maritima
2.3.1.31 0.18
-
 glutaryl-CoA 25°C, pH 7.5 Thermotoga maritima
2.3.1.31 0.24
-
 L-homoserine 25°C, pH 7.5 Thermotoga maritima
2.3.1.31 1.7
-
 O-acetylhomoserine 25°C, pH 7.5 Thermotoga maritima
2.3.1.31 280
-
 D-homoserine 25°C, pH 7.5 Thermotoga maritima

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.31 Thermotoga maritima
-
-
-
2.5.1.48 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.31
-
 Thermotoga maritima

Reaction

EC Number Reaction Comment Organism Reaction ID
2.3.1.31 acetyl-CoA + L-homoserine = CoA + O-acetyl-L-homoserine enzyme utilizes a ping-pong kinetic mechanism in which the acetate group of acetyl-CoA is initially transferred to an enzyme nucleophile before subsequent transfer to homoserine Thermotoga maritima
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.31 acetyl-CoA + D-homoserine
-
 Thermotoga maritima CoA + O-acetyl-D-homoserine
-
 ?
2.3.1.31 acetyl-CoA + L-homoserine the enzyme utilizes a ping-pong kinetic mechanism in which the acetate group of acetyl-CoA is initially transferred to an enzyme nucleophile before subsequent transfer to homoserine Thermotoga maritima CoA + O-acetyl-L-homoserine
-
 r
2.3.1.31 beta-hydroxybutyryl-CoA + L-homoserine
-
 Thermotoga maritima CoA + beta-hydroxybutyryl-L-homoserine
-
 ?
2.3.1.31 glutaryl-CoA + L-homoserine
-
 Thermotoga maritima CoA + O-glutaryl-L-homoserine
-
 ?
2.3.1.31 isobutyryl-CoA + L-homoserine
-
 Thermotoga maritima CoA + O-isobutyryl-L-homoserine
-
 ?
2.3.1.31 malonyl-CoA + L-homoserine
-
 Thermotoga maritima CoA + O-malonyl-L-homoserine
-
 ?
2.3.1.31 propionyl-CoA + L-homoserine
-
 Thermotoga maritima CoA + O-propionyl-L-homoserine
-
 ?
2.3.1.31 succinyl-CoA + L-homoserine
-
 Thermotoga maritima CoA + O-succinyl-L-homoserine
-
 ?
2.5.1.48 O-acetyl-L-homoserine + L-cysteine
-
 Escherichia coli L-cystathionine + acetate
-
 ?
2.5.1.48 O-succinyl-L-homoserine + L-cysteine
-
 Escherichia coli L-cystathionine + succinate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.31 TmHTS
-
 Thermotoga maritima

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.31 60 70
-
 Thermotoga maritima

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3.1.31 0.12
-
 malonyl-CoA 25°C, pH 7.5 Thermotoga maritima
2.3.1.31 0.12
-
 beta-hydroxybutyryl-CoA 25°C, pH 7.5 Thermotoga maritima
2.3.1.31 0.19
-
 succinyl-CoA 25°C, pH 7.5 Thermotoga maritima
2.3.1.31 0.19
-
 isobutyryl-CoA 25°C, pH 7.5 Thermotoga maritima
2.3.1.31 0.25
-
 glutaryl-CoA 25°C, pH 7.5 Thermotoga maritima
2.3.1.31 1.9
-
 CoA 25°C, pH 7.5 Thermotoga maritima
2.3.1.31 1.9
-
 O-acetylhomoserine 25°C, pH 7.5 Thermotoga maritima
2.3.1.31 2.6
-
 propionyl-CoA 25°C, pH 7.5 Thermotoga maritima
2.3.1.31 16
-
 D-homoserine 25°C, pH 7.5 Thermotoga maritima
2.3.1.31 23
-
 acetyl-CoA 25°C, pH 7.5 Thermotoga maritima
2.3.1.31 23
-
 L-homoserine 25°C, pH 7.5 Thermotoga maritima

Cofactor

EC Number Cofactor Comment Organism Structure
2.5.1.48 pyridoxal 5'-phosphate dependent Escherichia coli