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Literature summary extracted from

  • Hamerski, D.; Matern, U.
    Elicitor-induced biosynthesis of psoralens in Ammi majus L. suspension cultures. Microsomal conversion of demethylsuberosin into (+)marmesin and psoralen (1988), Eur. J. Biochem., 171, 369-375.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.14.14.141 additional information elicitor preparations from either Alternaria carthami Chowdhury or Phytophthora megasperma f.sp. glycinea induce the production of linear furanocoumarins in Ammi majus suspension culture Ammi majus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.14.141 Ancymidole 13% inhibition of the psoralen synthase at 0.05 mM Ammi majus
1.14.14.141 BAS 110 72% inhibition of the psoralen synthase at 0.05 mM, 38% at 0.005 mM Ammi majus
1.14.14.141 BAS 111 complete inhibition of the psoralen synthase at 0.001 mM, 86% at 0.0002 mM Ammi majus
1.14.14.141 BAS 978 complete inhibition of the psoralen synthase at 0.05 mM, 68% at 0.005 mM Ammi majus
1.14.14.141 ketoconazole complete inhibition of the psoralen synthase at 0.001 mM, 74% at 0.0002 mM Ammi majus
1.14.14.141 Tetcyclacis 85% inhibition of the psoralen synthase at 0.001 mM, 25% at 0.0002 mM Ammi majus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.14.141 endoplasmic reticulum lumen
-
Ammi majus 5788
-
1.14.14.141 microsome
-
Ammi majus
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.14.141 (+)-marmesin + [reduced NADPH-hemoprotein reductase] + O2 Ammi majus the substrate is synthesized from demethylsuberosin by the marmesin synthase, biosynthesis of furanocoumarin phytoalexin which are then excreted from the endoplasmic reticulum lumen into the cell culture medium psoralen + [oxidized NADPH-hemoprotein reductase] + acetone + 2 H2O
-
?
1.14.14.141 additional information Ammi majus umbelliferone is a better precursor of psoralens than either demethylsuberosin or marmesin ?
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.141 Ammi majus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.14.141 native enzyme partially by preparation of microsomes Ammi majus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.14.141 cell suspension culture
-
Ammi majus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.141 (+)-marmesin + [reduced NADPH-hemoprotein reductase] + O2
-
Ammi majus psoralen + [oxidized NADPH-hemoprotein reductase] + acetone + 2 H2O
-
?
1.14.14.141 (+)-marmesin + [reduced NADPH-hemoprotein reductase] + O2 the substrate is synthesized from demethylsuberosin by the marmesin synthase, biosynthesis of furanocoumarin phytoalexin which are then excreted from the endoplasmic reticulum lumen into the cell culture medium Ammi majus psoralen + [oxidized NADPH-hemoprotein reductase] + acetone + 2 H2O
-
?
1.14.14.141 additional information umbelliferone is a better precursor of psoralens than either demethylsuberosin or marmesin Ammi majus ?
-
?
1.14.14.141 additional information the psoralen synthase is a cytochrome-P450-dependent monooxygenase Ammi majus ?
-
?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.14.141 20
-
assay at Ammi majus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.14.141 7
-
assay at Ammi majus

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.14.141 additional information the psoralen synthase is a cytochrome-P450-dependent monooxygenase Ammi majus