Any feedback?
Literature summary extracted from
- Kinetics of mercury reduction by Serratia marcescens mercuric reductase expressed by Pseudomonas putida strains (2005), Eng. Life Sci., 5, 415-424.
No PubMed abstract available
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 1.16.1.1 | additional information | constitutive expression of enzyme in Pseudomonas putida results in a broad spectrum mercury resistance | Serratia marcescens |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.16.1.1 | Cl- | inhibitory above 0.1 M, complete inhibition at 1 M | Serratia marcescens |  |
| 1.16.1.1 | Hg2+ | pronounced substrate inhibition | Serratia marcescens | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.16.1.1 | 0.014 | - |
Hg2+ | pH 7.2, 30°C, detailed kinetics | Serratia marcescens | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.16.1.1 | 65000 | - |
3 * 65000, SDS-PAGE | Serratia marcescens |
| 1.16.1.1 | 190000 | - |
gel filtration | Serratia marcescens |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.16.1.1 | Serratia marcescens | - |
expressed by Pseudomonas putida | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.16.1.1 | recombinant enzyme | Serratia marcescens |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.16.1.1 | Hg2+ + NADPH | - |
Serratia marcescens | Hg + NADP+ + H+ | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.16.1.1 | trimer | 3 * 65000, SDS-PAGE | Serratia marcescens |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.16.1.1 | 37 | - |
- |
Serratia marcescens |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.16.1.1 | 7 | 7.8 | - |
Serratia marcescens |
pI Value
| EC Number | Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|---|
| 1.16.1.1 | Serratia marcescens | isoelectric focusing | - |
5.1 |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
