Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Srisawasdi, P.; Jearanaikoon, P.; Wetprasit, N.; Sriwanthana, B.; Kroll, M.H.; Lolekha, P.H.
    Application of Streptomyces and Brevibacterium cholesterol oxidase for total serum cholesterol assay by the enzymatic kinetic method (2006), Clin. Chim. Acta, 372, 103-111.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.1.3.6 diagnostics the enzyme is used for a kinetic cholesterol assay for determination of cholesterol content in human serum, overview, the enzyme from Streptomyces is more effective than the enzyme from Brevibacterium Streptomyces sp.
1.1.3.6 diagnostics the enzyme is used for a kinetic cholesterol assay for determination of cholesterol content in human serum, overview, the enzyme from Streptomyces is more effective than the enzyme from Brevibacterium Brevibacterium sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.3.6 additional information
-
 additional information kinetics, modelling Streptomyces sp.
1.1.3.6 additional information
-
 additional information kinetics, modelling Brevibacterium sp.

Organism

EC Number Organism UniProt Comment Textmining
1.1.3.6 Brevibacterium sp.
-
-
-
1.1.3.6 Streptomyces sp.
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.3.6 additional information
-
-
 Streptomyces sp.
1.1.3.6 additional information
-
-
 Brevibacterium sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.3.6 cholesterol + O2
-
 Streptomyces sp. cholest-4-en-3-one + H2O2
-
 ?
1.1.3.6 cholesterol + O2
-
 Brevibacterium sp. cholest-4-en-3-one + H2O2
-
 ?