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Literature summary extracted from

  • Celik, A.; Speight, R.E.; Turner, N.J.
    Identification of broad specificity P450CAM variants by primary screening against indole as substrate (2005), Chem. Commun. (Camb. ), 2005, 3652-3654.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
1.14.15.1 Y96A site-directed mutagenesis, the mutant gains the ability to hydroxylate indole to 3-hydroxyindole Pseudomonas putida
1.14.15.1 Y96C site-directed mutagenesis, the mutant gains the ability to hydroxylate indole to 3-hydroxyindole Pseudomonas putida
1.14.15.1 Y96G site-directed mutagenesis, the mutant gains the ability to hydroxylate indole to 3-hydroxyindole Pseudomonas putida
1.14.15.1 Y96M site-directed mutagenesis, the mutant gains the ability to hydroxylate indole to 3-hydroxyindole Pseudomonas putida
1.14.15.1 Y96Q site-directed mutagenesis, the mutant gains the ability to hydroxylate indole to 3-hydroxyindole Pseudomonas putida
1.14.15.1 Y96S site-directed mutagenesis, the mutant gains the ability to hydroxylate indole to 3-hydroxyindole Pseudomonas putida
1.14.15.1 Y96T site-directed mutagenesis, the mutant gains the ability to hydroxylate indole to 3-hydroxyindole Pseudomonas putida

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.15.1 (+)-camphor + O2 + reduced putidaredoxin Pseudomonas putida
-
 (+)-exo-5-hydroxycamphor + oxidized putidaredoxin + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.15.1 Pseudomonas putida
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.15.1 (+)-camphor + O2 + reduced putidaredoxin
-
 Pseudomonas putida (+)-exo-5-hydroxycamphor + oxidized putidaredoxin + H2O
-
 ?
1.14.15.1 indole + O2 + reduced putidaredoxin no substrate of the wild-type enzyme, but a good substrate for Y96 mutants, mutant screening, overview Pseudomonas putida 3-hydroxyindole + oxidized putidaredoxin + H2O 3-hydroxyindole undergoes spontaneous air oxidation to produce the insoluble dye indigo ?

Synonyms

EC Number Synonyms Comment Organism
1.14.15.1 P450cam
-
 Pseudomonas putida

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.15.1 cytochrome P450
-
 Pseudomonas putida
1.14.15.1 putidaredoxin
-
 Pseudomonas putida