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Literature summary extracted from

  • Bhatti, H.N.; Madeeha, M.; Asgher, M.; Batool, N.
    Purification and thermodynamic characterization of glucose oxidase from a newly isolated strain of Aspergillus niger (2006), Can. J. Microbiol., 52, 519-524.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.1.3.4 analysis the enzyme is useful in designing of biosensors for use in clinical, biochemical, and diagnostic assays Aspergillus niger

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.3.4 additional information
-
 additional information thermodynamics Aspergillus niger
1.1.3.4 25
-
 D-glucose pH 5.5, 40°C Aspergillus niger

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.3.4 D-glucose + O2 Aspergillus niger
-
 D-glucono-1,5-lactone + H2O2
-
 ?
1.1.3.4 D-glucose + O2 Aspergillus niger NFCCP
-
 D-glucono-1,5-lactone + H2O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.3.4 Aspergillus niger
-
-
-
1.1.3.4 Aspergillus niger NFCCP
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.3.4 native enzyme by ammonium sulfate fractionation, ion exchange chromatography, and gel filtration Aspergillus niger

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.3.4 mycelium
-
 Aspergillus niger
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.3.4 135
-
 purified enzyme Aspergillus niger

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.3.4 D-glucose + O2
-
 Aspergillus niger D-glucono-1,5-lactone + H2O2
-
 ?
1.1.3.4 D-glucose + O2 highly substrate specific enzyme Aspergillus niger D-glucono-1,5-lactone + H2O2
-
 ?
1.1.3.4 D-glucose + O2
-
 Aspergillus niger NFCCP D-glucono-1,5-lactone + H2O2
-
 ?
1.1.3.4 D-glucose + O2 highly substrate specific enzyme Aspergillus niger NFCCP D-glucono-1,5-lactone + H2O2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.3.4 GOD
-
 Aspergillus niger

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.3.4 40
-
-
 Aspergillus niger

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.3.4 40
-
 purified enzyme, half-life: 30 min Aspergillus niger

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.3.4 5.5
-
-
 Aspergillus niger