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Literature summary extracted from

  • Percy, M.J.; Crowley, L.J.; Roper, D.; Vulliamy, T.J.; Layton, D.M.; Barber, M.J.
    Identification and characterization of the novel FAD-binding lobe G75S mutation in cytochrome b(5) reductase: an aid to determine recessive congenital methemoglobinemia status in an infant (2006), Blood Cells Mol. Dis., 36, 81-90.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
1.6.2.2 G75S natural mutation isolated in patient with recessive congenital methemoglobinaemia. Mutant retains stoichiometric levels of FAD, but shows decreased catalytic efficiency and reduced protein stability Homo sapiens
1.6.2.2 G75S/V252M natural mutation isolated in patient with recessive congenital methemoglobinaemia. Mutant retains stoichiometric levels of FAD, but shows decreased catalytic efficiency and reduced protein stability Homo sapiens
1.6.2.2 V252M natural mutation isolated in patient with recessive congenital methemoglobinaemia. Mutant retains stoichiometric levels of FAD, but shows decreased catalytic efficiency and reduced protein stability Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.6.2.2 0.006
-
 NADH wild-type Homo sapiens
1.6.2.2 0.006
-
 ferricyanide wild-type Homo sapiens
1.6.2.2 0.007
-
 ferricyanide mutant G75S Homo sapiens
1.6.2.2 0.007
-
 ferricyanide mutant V252M Homo sapiens
1.6.2.2 0.008
-
 ferricyanide mutant G75S/V252M Homo sapiens
1.6.2.2 0.009
-
 NADH mutant G75S Homo sapiens
1.6.2.2 0.013
-
 ferricytochrome b5 wild-type Homo sapiens
1.6.2.2 0.013
-
 ferricytochrome b5 mutant V252M Homo sapiens
1.6.2.2 0.014
-
 ferricytochrome b5 mutant G75S Homo sapiens
1.6.2.2 0.014
-
 ferricytochrome b5 mutant G75S/V252M Homo sapiens
1.6.2.2 0.017
-
 NADH mutant G75S/V252M Homo sapiens
1.6.2.2 0.052
-
 NADH mutant V252M Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
1.6.2.2 Homo sapiens
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.6.2.2 2 ferricyanide + NADH
-
 Homo sapiens 2 ferrocyanide + NAD+ + H+
-
 ?
1.6.2.2 2 ferricytochrome b5 + NADH
-
 Homo sapiens 2 ferrocytochrome b5 + NAD+ + H+
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.6.2.2 60
-
 ferricytochrome b5 mutant G75S Homo sapiens
1.6.2.2 85
-
 ferricytochrome b5 mutant G75S/V252M Homo sapiens
1.6.2.2 133
-
 ferricyanide mutant G75S Homo sapiens
1.6.2.2 187
-
 ferricyanide mutant G75S/V252M Homo sapiens
1.6.2.2 285
-
 ferricytochrome b5 mutant V252M Homo sapiens
1.6.2.2 360
-
 ferricytochrome b5 wild-type Homo sapiens
1.6.2.2 633
-
 ferricyanide mutant V252M Homo sapiens
1.6.2.2 800
-
 ferricyanide wild-type Homo sapiens