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Literature summary extracted from

  • Carvajal-Vallejos, P.K.; Campos, A.; Fuentes-Prior, P.; Villalobos, E.; Almeida, A.M.; Barbera, E.; Torne, J.M.; Santos, M.
    Purification and in vitro refolding of maize chloroplast transglutaminase over-expressed in Escherichia coli (2007), Biotechnol. Lett., 29, 1255-1262.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.2.13 sub-cloned into a pET28 vector and overexpressed in Escherichia coli BL21. The recombinant proteins are present mainly as insoluble inclusion bodies. It can be effectively renatured in vitro and exhibits general TG enzyme characteristics Zea mays

Protein Variants

EC Number Protein Variants Comment Organism
2.3.2.13 production overexpression of enzyme in Escherichia coli and purification from inclusion bodies, refolding by rapid dilution in a Ca2+- and guanidine-containing buffer. Purified enzyme has similar characteristics as native protein Zea mays

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.2.13 Boc-DON-Gln-Ile-Val-OMe
-
 Zea mays
2.3.2.13 GTP
-
 Zea mays
2.3.2.13 monodansyl cadaverine
-
 Zea mays

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.2.13 chloroplast
-
 Zea mays 9507
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.2.13 55000
-
 x * 55000, SDS-PAGE Zea mays

Organism

EC Number Organism UniProt Comment Textmining
2.3.2.13 Zea mays
-
-
-
2.3.2.13 Zea mays
-
 patent WWO03102128, expression in Escherichia coli
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.2.13
-
 Zea mays
2.3.2.13 overexpression of enzyme in Escherichia coli and purification from inclusion bodies, refolding by rapid dilution in a Ca2+- and guanidine-containing buffer Zea mays

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.2.13 N,N-dimethylcasein + putrescine
-
 Zea mays ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.2.13 ? x * 55000, SDS-PAGE Zea mays

Synonyms

EC Number Synonyms Comment Organism
2.3.2.13 TGZ
-
 Zea mays