EC Number | Application | Comment | Organism |
---|---|---|---|
1.14.15.1 | analysis | the enzyme is useful in whole cell biocatalyst systems | Pseudomonas putida |
1.14.15.1 | synthesis | the enzyme is useful in whole cell biocatalyst systems | Pseudomonas putida |
EC Number | Cloned (Comment) | Organism |
---|---|---|
1.14.15.1 | expression of enzyme and mutant cofactor in Escherichia coli strain BL21(DE3) | Pseudomonas putida |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.14.15.1 | additional information | construction of a catalytically active recombinant Escherichia coli whole cell biocatalyst harboring a cytochrome P450cam monooxygenase system from Pseudomonas putida coupled with enzymatic cofactor putidaredoxin mutant C73S/C85S regeneration, the cofactor mutant is 2fold more effective than the wild-type putidaredoxin | Pseudomonas putida |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.15.1 | (+)-camphor + O2 + reduced putidaredoxin | Pseudomonas putida | - |
(+)-exo-5-hydroxycamphor + oxidized putidaredoxin + H2O | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.15.1 | Pseudomonas putida | - |
- |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.15.1 | (+)-camphor + O2 + reduced putidaredoxin | - |
Pseudomonas putida | (+)-exo-5-hydroxycamphor + oxidized putidaredoxin + H2O | - |
? | |
1.14.15.1 | (+)-camphor + O2 + reduced putidaredoxin | regio- and stereospecific hydroxylation | Pseudomonas putida | (+)-exo-5-hydroxycamphor + oxidized putidaredoxin + H2O | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.14.15.1 | cytochrome p450cam | - |
Pseudomonas putida |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.14.15.1 | 37 | - |
in vivo activity | Pseudomonas putida |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.14.15.1 | additional information | - |
temperature profile | Pseudomonas putida |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.14.15.1 | 7.4 | - |
in vivo and in vitro pH optimum | Pseudomonas putida |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
1.14.15.1 | additional information | - |
pH profile | Pseudomonas putida |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.14.15.1 | NADH | is required for O2 activation | Pseudomonas putida | |
1.14.15.1 | putidaredoxin | wild-type and mutant C73S/C85S cofactor, the mutant shows about 2fold improved substrate conversion | Pseudomonas putida |